利用 Box-Behnken 设计优化从 Fucus vesiculosus 中提取叶绿素:揭示增强抗氧化活性和抑制代谢酶的技术

IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2024-09-22 DOI:10.1016/j.algal.2024.103723
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The UAE method, under its optimal conditions, yielded higher values: 954.00 ± 33.65 mg PGE/100 g, 146.35 ± 3.30 mg TE/100 g, 1037.61 ± 32.93 mg TE/100 g, and 809.95 ± 46.58 mg TE/100 g. The extracts were tested for inhibitory effects on α-amylase and α-glucosidase, with the purified UAE extract showing greater inhibition of α-amylase (IC<sub>50</sub> = 7.06 ± 0.67 μg/mL) compared to acarbose (IC<sub>50</sub> = 14.63 ± 0.28 μg/mL). For α-glucosidase, acarbose had higher activity (IC<sub>50</sub> = 11.54 ± 0.04 μg/mL) than the UAE purified extract (IC<sub>50</sub> = 15.04 ± 0.07 μg/mL). UHPLC-qTOF-MS analysis identified various phlorotannin derivatives, with Tetrafucotetraphlorethol (<em>m</em>/<em>z</em> 993) only in the UAE purified extract. 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引用次数: 0

摘要

绿单宁是一种存在于棕色大型藻类中的酚类化合物,具有潜在的生物活性作用。本研究评估了采用传统和超声辅助萃取(UAE)方法从岩藻中萃取绿单宁的情况,重点关注 UAE 的溶剂-材料比、溶剂浓度、萃取时间和超声功率。实验采用 Box-Behnken 设计,测量了总单宁含量(TPhC)、DPPH、FRAP 和 ABTS。在最佳条件下,传统方法产生的 TPhC、DPPH、FRAP 和 ABTS 值分别为 512.25 ± 14.26 毫克 PGE/100 克、29.70 ± 1.40 毫克 TE/100 克、589.57 ± 15.06 毫克 TE/100 克和 332.73 ± 31.03 毫克 TE/100 克。在最佳条件下,阿联酋方法得到了更高的数值:954.00 ± 33.65 mg PGE/100 g、146.35 ± 3.30 mg TE/100 g、1037.61 ± 32.93 mg TE/100 g 和 809.95 ± 46.58 mg TE/100 g。测试了提取物对α-淀粉酶和α-葡萄糖苷酶的抑制作用,与阿卡波糖(IC50 = 14.63 ± 0.28 μg/mL)相比,纯化的 UAE 提取物对α-淀粉酶的抑制作用更大(IC50 = 7.06 ± 0.67 μg/mL)。对于α-葡萄糖苷酶,阿卡波糖的活性(IC50 = 11.54 ± 0.04 μg/mL)高于阿联酋纯化提取物(IC50 = 15.04 ± 0.07 μg/mL)。超高效液相色谱-qTOF-MS分析鉴定出了多种绿丹宁衍生物,其中只有阿联酋纯化提取物中含有四桉叶油醇(m/z 993)。这项研究的结论是,从阿联酋纯化的叶单宁提取物有可能调节α-淀粉酶和α-葡萄糖苷酶,这些酶与糖尿病和肥胖症等代谢紊乱有关。
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Optimizing phlorotannins extraction from Fucus vesiculosus using Box-Behnken design: Unveiling techniques for enhanced antioxidant activity and metabolic enzyme inhibition
Phlorotannins, phenolic compounds found in brown macroalgae, exhibit potential bioactive effects. This study evaluated the extraction of phlorotannins from Fucus vesiculosus using conventional and ultrasound-assisted extraction (UAE) methods, focusing on solvent-material ratio, solvent concentration, extraction time, and sonication power for UAE. The experiments, designed using a Box-Behnken design, measured total phlorotannin content (TPhC), DPPH, FRAP, and ABTS. The conventional method, under optimal conditions, produced TPhC, DPPH, FRAP, and ABTS values of 512.25 ± 14.26 mg PGE/100 g, 29.70 ± 1.40 mg TE/100 g, 589.57 ± 15.06 mg TE/100 g, and 332.73 ± 31.03 mg TE/100 g, respectively. The UAE method, under its optimal conditions, yielded higher values: 954.00 ± 33.65 mg PGE/100 g, 146.35 ± 3.30 mg TE/100 g, 1037.61 ± 32.93 mg TE/100 g, and 809.95 ± 46.58 mg TE/100 g. The extracts were tested for inhibitory effects on α-amylase and α-glucosidase, with the purified UAE extract showing greater inhibition of α-amylase (IC50 = 7.06 ± 0.67 μg/mL) compared to acarbose (IC50 = 14.63 ± 0.28 μg/mL). For α-glucosidase, acarbose had higher activity (IC50 = 11.54 ± 0.04 μg/mL) than the UAE purified extract (IC50 = 15.04 ± 0.07 μg/mL). UHPLC-qTOF-MS analysis identified various phlorotannin derivatives, with Tetrafucotetraphlorethol (m/z 993) only in the UAE purified extract. This study concludes that purified phlorotannin extract from UAE can potentially regulate α-amylase and α-glucosidase, enzymes linked to metabolic disorders like diabetes and obesity.
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来源期刊
Algal Research-Biomass Biofuels and Bioproducts
Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
9.40
自引率
7.80%
发文量
332
期刊介绍: Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment
期刊最新文献
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