丹酚酸 B 通过抑制 TRPC6 和 TGF-β/Smad3 通路抑制糖尿病心肌病过程中的心肌纤维化

IF 3.5 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Food Biochemistry Pub Date : 2024-09-27 DOI:10.1155/2024/5525825
Pengyu Lu, Meng Zhang, Zhaoyang Chen, QiYao Xu, Min Liu, Fusen Zhao, Xuan Liu, Xindong Wang
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引用次数: 0

摘要

丹参中的主要水溶性多酚成分丹酚酸 B(Sal B)具有抗炎、抗氧化和抗细胞凋亡的作用,尤其是在保护心血管方面。然而,盐 B 影响心肌纤维化的机制还需要进一步研究。在体内,我们通过高脂饮食和腹腔注射链脲佐菌素(STZ)建立了糖尿病小鼠模型。然后用 Sal B、瞬时受体电位通道 6(TRPC6)诱导剂或它们的组合处理小鼠。TRPC6 的上调会加重心肌病变,导致心肌肥厚和胶原纤维沉积。在体外,转化生长因子(TGF)-β1诱导心脏成纤维细胞向肌成纤维细胞转分化,形成肌成纤维细胞模型。Sal B、TRPC6 诱导剂或它们的组合。TRPC6 的上调增加了 I 型胶原 C 端丙肽(PICP)和 III 型胶原 N 端丙肽(PIIINP)的分泌,促进了肌成纤维细胞的增殖和迁移。我们的研究表明,TRPC6在心肌纤维化中表达上调,增强了TGF-β/Smad3信号传导,促进了胶原蛋白I(COL-1)的合成。Sal B抑制了TRPC6的异常表达和TGF-β/Smad3的激活,减轻了这些影响。因此,Sal B可通过调节TRPC6的表达和TGF-β/Smad3信号通路缓解糖尿病患者的心肌纤维化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Salvianolic Acid B Inhibits Myocardial Fibrosis during Diabetic Cardiomyopathy via Suppressing TRPC6 and TGF-β/Smad3 Pathway

Salvianolic acid B (Sal B), the main water-soluble polyphenolic constituent of Danshen, is noted for its anti-inflammatory, antioxidant, and antiapoptotic properties, particularly in cardiovascular protection. However, the mechanisms by which Sal B affects myocardial fibrosis require further investigation. In vivo, we established a diabetic mouse model using a high-fat diet and intraperitoneal streptozotocin (STZ) administration. Mice were then treated with Sal B, the transient receptor potential channel 6 (TRPC6) inducers, or their combination. Upregulation of TRPC6 worsened myocardial pathology, leading to cardiac hypertrophy and collagen fiber deposition. In vitro, transforming growth factor (TGF)-β1 induced transdifferentiation of cardiac fibroblasts into myofibroblasts, creating a myofibroblast cell model. Sal B, TRPC6 inducers, or their combination were administered. TRPC6 upregulation increased procollagen type I C-terminal propeptide (PICP) and procollagen type III N-terminal propeptide (PIIINP) secretion, promoting myofibroblast proliferation and migration. Our study indicates that TRPC6 expression is upregulated in myocardial fibrosis, enhancing TGF-β/Smad3 signaling and promoting collagen I (COL-1) synthesis. Sal B inhibited abnormal TRPC6 expression and TGF-β/Smad3 activation, mitigating these effects. Thus, Sal B alleviates myocardial fibrosis in diabetes by modulating TRPC6 expression and TGF-β/Smad3 signaling pathway.

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来源期刊
Journal of Food Biochemistry
Journal of Food Biochemistry 生物-生化与分子生物学
CiteScore
7.80
自引率
5.00%
发文量
488
审稿时长
3.6 months
期刊介绍: The Journal of Food Biochemistry publishes fully peer-reviewed original research and review papers on the effects of handling, storage, and processing on the biochemical aspects of food tissues, systems, and bioactive compounds in the diet. Researchers in food science, food technology, biochemistry, and nutrition, particularly based in academia and industry, will find much of great use and interest in the journal. Coverage includes: -Biochemistry of postharvest/postmortem and processing problems -Enzyme chemistry and technology -Membrane biology and chemistry -Cell biology -Biophysics -Genetic expression -Pharmacological properties of food ingredients with an emphasis on the content of bioactive ingredients in foods Examples of topics covered in recently-published papers on two topics of current wide interest, nutraceuticals/functional foods and postharvest/postmortem, include the following: -Bioactive compounds found in foods, such as chocolate and herbs, as they affect serum cholesterol, diabetes, hypertension, and heart disease -The mechanism of the ripening process in fruit -The biogenesis of flavor precursors in meat -How biochemical changes in farm-raised fish are affecting processing and edible quality
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