利用细胞异体移植物进行体内软骨结构和表面润滑的综合修复。

IF 6.4 1区 医学 Q1 CELL & TISSUE ENGINEERING npj Regenerative Medicine Pub Date : 2024-09-28 DOI:10.1038/s41536-024-00367-x
Jeanne E Barthold, Luyao Cai, Kaitlin P McCreery, Kristine M Fischenich, Kevin N Eckstein, Virginia L Ferguson, Nancy C Emery, Gert Breur, Corey P Neu
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引用次数: 0

摘要

关节软骨损伤后的修复具有挑战性,而脱细胞组织为促进再生提供了一种可能的治疗方案。在这里,我们的研究表明,在绵羊模型中,与未经处理的缺损相比,细胞骨软骨异体移植在6个月后能改善整合软骨修复。通过核磁共振成像评估组织内应变/结构的功能性测量结果表明,植入物和原生软骨具有相似的生物力学特性。与原生组织和缺损相比,细胞异体移植物的结构、组成和摩擦学保持了表面粗糙度和润滑性、压缩和松弛下的材料特性、胶原:糖胺聚糖和胶原:磷酸盐的组成比例以及 I/II 型胶原的相对组成。虽然在骨区域和软骨异体移植物之间的整合区观察到了高细胞性,但软骨植入物的再细胞化并不一致,细胞迁移到致密脱细胞组织的距离通常小于 ~750 µm,这可能限制了细胞的长期维持。我们的研究结果表明,无细胞同种异体移植物在体内至少有六个月的结构和生物力学功效。
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Integrative cartilage repair using acellular allografts for engineered structure and surface lubrication in vivo.

The repair of articular cartilage after damage is challenging, and decellularized tissue offers a possible treatment option to promote regeneration. Here, we show that acellular osteochondral allografts improve integrative cartilage repair compared to untreated defects after 6 months in an ovine model. Functional measures of intratissue strain/structure assessed by MRI demonstrate similar biomechanics of implants and native cartilage. Compared to native tissue and defects, the structure, composition, and tribology of acellular allografts preserve surface roughness and lubrication, material properties under compression and relaxation, compositional ratios of collagen:glycosaminoglycan and collagen:phosphate, and relative composition of types I/II collagen. While high cellularity was observed in bone regions and integration zones between cartilage-allografts, recellularization of chondral implants was inconsistent, with cell migration typically less than ~750 µm into the dense decellularized tissue, possibly limiting long-term cellular maintenance. Our results demonstrate the structural and biomechanical efficacy of acellular allografts for at least six months in vivo.

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来源期刊
npj Regenerative Medicine
npj Regenerative Medicine Engineering-Biomedical Engineering
CiteScore
10.00
自引率
1.40%
发文量
71
审稿时长
12 weeks
期刊介绍: Regenerative Medicine, an innovative online-only journal, aims to advance research in the field of repairing and regenerating damaged tissues and organs within the human body. As a part of the prestigious Nature Partner Journals series and in partnership with ARMI, this high-quality, open access journal serves as a platform for scientists to explore effective therapies that harness the body's natural regenerative capabilities. With a focus on understanding the fundamental mechanisms of tissue damage and regeneration, npj Regenerative Medicine actively encourages studies that bridge the gap between basic research and clinical tissue repair strategies.
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