Yu Zhang , Yaqi Cong , Juan Du , Donghua Guo , Jing Huang , Junchen Pan , Youde Liang , Jiali Zhang , Zhou Ye , Yi Liu , Yi Zhou
{"title":"缺乏 Lif 会导致全身性铁代谢紊乱,并增加成骨细胞对铁变态反应的易感性。","authors":"Yu Zhang , Yaqi Cong , Juan Du , Donghua Guo , Jing Huang , Junchen Pan , Youde Liang , Jiali Zhang , Zhou Ye , Yi Liu , Yi Zhou","doi":"10.1016/j.bone.2024.117266","DOIUrl":null,"url":null,"abstract":"<div><div>Leukemia inhibitory factor (LIF) is a multifunctional cytokine that plays a crucial role in various biological processes. However, LIF involvement in iron metabolism remains almost unexplored. This study aimed to explore the impact of LIF on systemic iron transportation and its potential role in ferroptosis in osteoblasts. We observed that the <em>Lif</em>-deficient (<em>Lif</em><sup><em>−/−</em></sup>) mice is characterized by a reduction in bone mass and a decrease in bone mineral density compared with wild-type (WT) mice. Energy-dispersive X-ray spectroscopy revealed a marked increase in iron content on the surface of femurs from <em>Lif</em><sup><em>−/−</em></sup> mice. Meanwhile, iron stores test lower iron levels in the spleens and higher levels in the femurs of <em>Lif</em><sup><em>−/−</em></sup> mice. Besides, <em>Lif</em><sup><em>−/−</em></sup> mice display increased levels of serum iron, total iron-binding capacity, unsaturated iron-binding capacity, and transferrin saturation and serum ferritin relative to WT mice. Hepcidin mRNA expression reduction in the liver of <em>Lif</em><sup><em>−/−</em></sup> mice. It also holds true in the AML-12 hepatocyte cell line after <em>Lif</em>-knockdown. Immunohistochemistry and RT-PCR revealed elevated ferroportin (FPN) in duodenal cells of <em>Lif</em><sup><em>−/−</em></sup> mice. <em>Lif</em>-deficiency decreases SLC7A11 levels in osteoblasts. In addition, overexpression of LIF downregulates CD71, DCYTB, and DMT1, thereby reducing iron uptake in iron-overloaded cells. Femur immunohistochemistry (IHC) revealed increased ACSL4 and decreased GPX4 and SLC7A11, indicating an increase in ferroptosis of osteoblasts in <em>Lif</em><sup><em>−/−</em></sup> mice. Whole-transcriptome sequencing showed gene expression changes after <em>Lif</em>-knockdown, exhibiting a negative correlation with genes involved in long-chain fatty acid transport, mitochondrial organization, and the p38 MAPK signaling pathway. These results demonstrate that <em>Lif</em>-deficiency alter systemic iron metabolism and increases the susceptibility of osteoblasts to ferroptosis.</div></div>","PeriodicalId":9301,"journal":{"name":"Bone","volume":"189 ","pages":"Article 117266"},"PeriodicalIF":3.5000,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Lif-deficiency promote systemic Iron metabolism disorders and increases the susceptibility of osteoblasts to ferroptosis\",\"authors\":\"Yu Zhang , Yaqi Cong , Juan Du , Donghua Guo , Jing Huang , Junchen Pan , Youde Liang , Jiali Zhang , Zhou Ye , Yi Liu , Yi Zhou\",\"doi\":\"10.1016/j.bone.2024.117266\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Leukemia inhibitory factor (LIF) is a multifunctional cytokine that plays a crucial role in various biological processes. However, LIF involvement in iron metabolism remains almost unexplored. This study aimed to explore the impact of LIF on systemic iron transportation and its potential role in ferroptosis in osteoblasts. We observed that the <em>Lif</em>-deficient (<em>Lif</em><sup><em>−/−</em></sup>) mice is characterized by a reduction in bone mass and a decrease in bone mineral density compared with wild-type (WT) mice. Energy-dispersive X-ray spectroscopy revealed a marked increase in iron content on the surface of femurs from <em>Lif</em><sup><em>−/−</em></sup> mice. Meanwhile, iron stores test lower iron levels in the spleens and higher levels in the femurs of <em>Lif</em><sup><em>−/−</em></sup> mice. Besides, <em>Lif</em><sup><em>−/−</em></sup> mice display increased levels of serum iron, total iron-binding capacity, unsaturated iron-binding capacity, and transferrin saturation and serum ferritin relative to WT mice. Hepcidin mRNA expression reduction in the liver of <em>Lif</em><sup><em>−/−</em></sup> mice. It also holds true in the AML-12 hepatocyte cell line after <em>Lif</em>-knockdown. Immunohistochemistry and RT-PCR revealed elevated ferroportin (FPN) in duodenal cells of <em>Lif</em><sup><em>−/−</em></sup> mice. <em>Lif</em>-deficiency decreases SLC7A11 levels in osteoblasts. In addition, overexpression of LIF downregulates CD71, DCYTB, and DMT1, thereby reducing iron uptake in iron-overloaded cells. Femur immunohistochemistry (IHC) revealed increased ACSL4 and decreased GPX4 and SLC7A11, indicating an increase in ferroptosis of osteoblasts in <em>Lif</em><sup><em>−/−</em></sup> mice. Whole-transcriptome sequencing showed gene expression changes after <em>Lif</em>-knockdown, exhibiting a negative correlation with genes involved in long-chain fatty acid transport, mitochondrial organization, and the p38 MAPK signaling pathway. These results demonstrate that <em>Lif</em>-deficiency alter systemic iron metabolism and increases the susceptibility of osteoblasts to ferroptosis.</div></div>\",\"PeriodicalId\":9301,\"journal\":{\"name\":\"Bone\",\"volume\":\"189 \",\"pages\":\"Article 117266\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bone\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S8756328224002552\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bone","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S8756328224002552","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
Lif-deficiency promote systemic Iron metabolism disorders and increases the susceptibility of osteoblasts to ferroptosis
Leukemia inhibitory factor (LIF) is a multifunctional cytokine that plays a crucial role in various biological processes. However, LIF involvement in iron metabolism remains almost unexplored. This study aimed to explore the impact of LIF on systemic iron transportation and its potential role in ferroptosis in osteoblasts. We observed that the Lif-deficient (Lif−/−) mice is characterized by a reduction in bone mass and a decrease in bone mineral density compared with wild-type (WT) mice. Energy-dispersive X-ray spectroscopy revealed a marked increase in iron content on the surface of femurs from Lif−/− mice. Meanwhile, iron stores test lower iron levels in the spleens and higher levels in the femurs of Lif−/− mice. Besides, Lif−/− mice display increased levels of serum iron, total iron-binding capacity, unsaturated iron-binding capacity, and transferrin saturation and serum ferritin relative to WT mice. Hepcidin mRNA expression reduction in the liver of Lif−/− mice. It also holds true in the AML-12 hepatocyte cell line after Lif-knockdown. Immunohistochemistry and RT-PCR revealed elevated ferroportin (FPN) in duodenal cells of Lif−/− mice. Lif-deficiency decreases SLC7A11 levels in osteoblasts. In addition, overexpression of LIF downregulates CD71, DCYTB, and DMT1, thereby reducing iron uptake in iron-overloaded cells. Femur immunohistochemistry (IHC) revealed increased ACSL4 and decreased GPX4 and SLC7A11, indicating an increase in ferroptosis of osteoblasts in Lif−/− mice. Whole-transcriptome sequencing showed gene expression changes after Lif-knockdown, exhibiting a negative correlation with genes involved in long-chain fatty acid transport, mitochondrial organization, and the p38 MAPK signaling pathway. These results demonstrate that Lif-deficiency alter systemic iron metabolism and increases the susceptibility of osteoblasts to ferroptosis.
期刊介绍:
BONE is an interdisciplinary forum for the rapid publication of original articles and reviews on basic, translational, and clinical aspects of bone and mineral metabolism. The Journal also encourages submissions related to interactions of bone with other organ systems, including cartilage, endocrine, muscle, fat, neural, vascular, gastrointestinal, hematopoietic, and immune systems. Particular attention is placed on the application of experimental studies to clinical practice.