光生物调节在糖尿病受损牙槽骨愈合中的比较研究:组织形态计量学和分子研究

Zohreh Dalirsani, Mina Davaji, Hamideh Salari Sedigh, Sara Hosseinian, Esmail Ranjbar, Afshin Yaqoubi, Kioumars Maraghe Moghaddam, Reyhaneh Shafieian
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引用次数: 0

摘要

目的:糖尿病在全球呈上升趋势。光生物调节(PBM)作为一种治疗方法被广泛应用于各种医疗领域。本研究旨在比较波长为 660、808 或 660 + 808 纳米的光生物调节对糖尿病大鼠牙槽骨愈合的影响。研究方法从糖尿病 Wistar 大鼠(n = 36)身上拔出双侧上颌第一磨牙。用波长为 660 nm 的 In-Ga-Al-P 激光器(7.2 J/cm2,24 s;DM660)、波长为 808 nm 的 Ga-Al-As 激光器(7 J/cm2,14 s;DM808)或这两组激光器的组合(DM-dual)对右侧牙槽进行处理(n = 12)。左侧为对照组。第 7 天或第 14 天,对标本进行组织形态计量学或实时 PCR 分析,以检测与 runt 相关的转录因子 2、骨钙蛋白、胶原 I 和血管内皮生长因子的表达。结果与 DM-660 相比,DM-808 组和 DM 双组的辐照牙套在骨组织和血管建立方面均有显著增加。此外,与其他组相比,DM-双组的纤维化组织量最少。结论在我们的研究范围内,本实验表明波长为 808 纳米的 PBM 可单独或与波长为 660 纳米的辐照相结合,促进糖尿病大鼠牙槽骨愈合,同时将纤维化诱导降到最低。
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Comparative Investigation of Photobiomodulation in Diabetes-Impaired Alveolar Bone Healing: A Histomorphometrical and Molecular Study.

Objective: Diabetes mellitus is increasing worldwide. Photobiomodulation (PBM) is proposed as a therapeutic method in various medical concerns. This study aimed to compare the effects of PBM at the wavelengths of 660, 808, or 660 + 808 nm on alveolar bone healing in diabetic rats. Methods: Bilateral maxillary first molars were extracted from diabetic Wistar rats (n = 36). Right-sided sockets were treated by an In-Ga-Al-P laser at 660 nm (7.2 J/cm2, 24 s; DM660), Ga-Al-As laser at 808 nm (7 J/cm2, 14 s; DM808), or a combination of these two sets (DM-dual) (n = 12). Left sides served as controls. On days 7 or 14, specimens were assigned for histomorphometric or real-time PCR analysis of runt-related transcription factor 2, osteocalcin, collagen I, and vascular endothelial growth factor expression. Results: Irradiated sockets of groups DM-808 and DM-dual showed a significant increase in bone tissue and blood vessel establishment as compared to DM-660. Further, group DM-dual exhibited the least amount of fibrotic tissue as compared to the other groups. Conclusions: Within our study limits, the present experiment suggested PBM at 808 nm, alone or combined with 660 nm irradiation, could promote alveolar bone healing, along with minimal fibrosis induction, in diabetic rats.

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期刊介绍: Photobiomodulation, Photomedicine, and Laser Surgery Editor-in-Chief: Michael R Hamblin, PhD Co-Editor-in-Chief: Heidi Abrahamse, PhD
期刊最新文献
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