接种物微生物质量与微生物产量呈负相关,而与体外甲烷产量呈正相关。

IF 2.4 Q3 NUTRITION & DIETETICS Journal of Nutritional Science Pub Date : 2024-09-20 eCollection Date: 2024-01-01 DOI:10.1017/jns.2024.37
Xiaoyu Zhang, Fenja Klevenhusen, Angela Sünder, Marcus Clauss, Jürgen Hummel
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引用次数: 0

摘要

瘤胃微生物主要将饲料碳水化合物分解为 SCFA、甲烷 (CH4) 和二氧化碳 (CO2),发酵终产物与微生物净增加量之间存在可预测的关系。我们使用封闭的体外批量培养系统培养青草和玉米青贮饲料,测量了 8 小时和 24 小时的总产气量、真正降解的基质、24 小时的 SCFA、CH4 净产量和微生物生物量,并研究了青贮饲料类型和接种物微生物质量对发酵方向的影响。微生物净产量与 8 小时内的气体总量呈负相关(P < 0-001),但与 24 小时内的气体总量无关(P = 0-052),与 CH4 产量呈负相关(P < 0-001)。较高的初始接种微生物量与较低的净微生物产量有关(P < 0-001),但与较高的 CH4 产量有关(P < 0-001)。在这些关系中,青贮草料和青贮玉米之间存在明显差异(P < 0-050)。青贮草的代谢氢(2H)回收率为 102.8 ± 12.3%,青贮玉米的代谢氢(2H)回收率为 118.8 ± 13.3%。总体而言,与玉米青贮相比,青草青贮更有利于基质转化为微生物生物量,而较少转化为发酵终产物。较低的接种物微生物量促进了更多的微生物生长,并且由于微生物合成的 2H 汇,减少了 CH4 的产生。
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Inoculum microbial mass is negatively related to microbial yield and positively to methane yield in vitro.

Ruminal microbes catabolise feed carbohydrates mainly into SCFA, methane (CH4), and carbon dioxide (CO2), with predictable relationships between fermentation end products and net microbial increase. We used a closed in vitro batch culture system, incubating grass and maize silages, and measured total gas production at 8 and 24 h, as well as the truly degraded substrate, the net production of SCFA, CH4, and microbial biomass at 24 h, and investigated the impact of silage type and inoculum microbial mass on fermentation direction. Net microbial yield was negatively correlated with total gas at 8 h (P < 0•001), but not at 24 h (P = 0•052), and negatively correlated with CH4 production (P < 0•001). Higher initial inoculum microbial mass was related to a lower net microbial yield (P < 0•001) but a higher CH4 production (P < 0•001). A significant difference between grass silage and maize silage was detected within the context of these relationships (P < 0•050). The metabolic hydrogen (2H) recovery was 102.8 ± 12.3 % for grass silages and 118.8 ± 13.3% for maize silages. Overall, grass silages favoured more substrate conversion to microbial biomass and less to fermentation end products than maize silage. Lower inoculum microbial mass facilitated more microbial growth and, because of the 2H sink by microbial synthesis, decreased CH4 production.

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来源期刊
Journal of Nutritional Science
Journal of Nutritional Science NUTRITION & DIETETICS-
CiteScore
3.00
自引率
0.00%
发文量
91
审稿时长
7 weeks
期刊介绍: Journal of Nutritional Science is an international, peer-reviewed, online only, open access journal that welcomes high-quality research articles in all aspects of nutrition. The underlying aim of all work should be, as far as possible, to develop nutritional concepts. JNS encompasses the full spectrum of nutritional science including public health nutrition, epidemiology, dietary surveys, nutritional requirements, metabolic studies, body composition, energetics, appetite, obesity, ageing, endocrinology, immunology, neuroscience, microbiology, genetics, molecular and cellular biology and nutrigenomics. JNS welcomes Primary Research Papers, Brief Reports, Review Articles, Systematic Reviews, Workshop Reports, Letters to the Editor and Obituaries.
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