睡茄对缺血性中风的神经保护作用是通过抗炎反应和调节神经递质水平来实现的。

IF 4.4 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Neurochemistry international Pub Date : 2024-09-28 DOI:10.1016/j.neuint.2024.105867
Abhilasha Sood , Arpit Mehrotra , Devinder K. Dhawan , Rajat Sandhir
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引用次数: 0

摘要

本研究旨在评估薇甘菊(WS)水醇根提取物对大脑中动脉闭塞(MCAO)诱导的缺血再灌注损伤(IRI)的有益作用。MCAO 动物的 mRNA 和蛋白水平显示 IL-6、TNF-α 和 MCP-1 水平升高。同时,MCAO动物的星形胶质细胞和小胶质细胞活化标记物(GFAP和IBA-1)的mRNA和蛋白质水平也有所升高。MCAO动物大脑中的COX-2和NF-kβ蛋白水平也有所增加。神经递质谷氨酸和 GABA 的水平在 MCAO 动物中有所增加。相反,MCAO 动物体内儿茶酚胺、多巴胺、去甲肾上腺素和血清素的水平降低。此外,MCAO 动物的运动活动减少。然而,在 MCAO 动物体内预先补充 300 毫克/千克(体重)剂量的 WS 水醇根提取物可减少 IL-6、TNF-α 和 MCP-1 的表达。此外,与 MCAO 动物相比,WS 还能减少 GFAP 和 Iba-1 阳性细胞的数量。观察还发现,预先补充 WS 可抑制 MCAO 诱导的 COX-2、NF-kβ 蛋白的增加,并降低谷氨酸的水平。在预先补充 WS 的 MCAO 动物中,GABA、多巴胺、去甲肾上腺素和血清素的水平均有所提高。预先补充 WS 还能防止 MCAO 动物出现运动障碍。综上所述,这些研究结果表明,WS 能有效减轻 IRI 诱导的神经炎症、神经化学物质改变和缺血性中风 MCAO 模型的运动障碍,从而表明其在缺血再灌注损伤中的改善作用。
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Neuroprotective effects of Withania somnifera on ischemic stroke are mediated via anti-inflammatory response and modulation of neurotransmitter levels
The present study was designed to evaluate the beneficial effects of hydroalcoholic root extract of Withania somnifera (WS) on ischemia-reperfusion injury (IRI) induced by Middle Cerebral Artery Occlusion (MCAO). MCAO animals showed increase in IL-6, TNF-α and MCP-1 levels in terms of mRNA and protein levels. Concomitantly, mRNA and protein levels for astrocyte and microglial activation markers; GFAP and IBA-1, were increased in MCAO animals. COX-2 and NF-kβ protein levels were also increased in the brains of MCAO animals. The levels of neurotransmitters; glutamate and GABA were increased in the MCAO animals. On the contrary, levels of catecholamines; dopamine, norepinephrine and serotonin were reduced in the MCAO animals. Additionally, MCAO animals showed reduced locomotor activity. However, pre-supplementation with WS hydro-alcoholic root extract at a dose of 300 mg/kg, body weight to MCAO animals reduced the expression of IL-6, TNF-α and MCP-1. In addition, WS also reduced the number of GFAP and Iba-1 positive cells in comparison to MCAO animals. WS pre-supplementation was also observed to inhibit MCAO induced increase in COX-2; NF-kβ proteins and reduce the glutamate levels. The levels of GABA, dopamine, norepinephrine and serotonin were increased in WS pre-supplemented MCAO animals. WS pre-supplementation also prevented motor deficits in the MCAO animals. Taken together, these findings suggest that WS is effective in attenuating IRI induced neuroinflammation, neurochemical alterations and motor deficits in MCAO model of ischemic stroke thereby suggesting its ameliorative role in ischemia-reperfusion injury.
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来源期刊
Neurochemistry international
Neurochemistry international 医学-神经科学
CiteScore
8.40
自引率
2.40%
发文量
128
审稿时长
37 days
期刊介绍: Neurochemistry International is devoted to the rapid publication of outstanding original articles and timely reviews in neurochemistry. Manuscripts on a broad range of topics will be considered, including molecular and cellular neurochemistry, neuropharmacology and genetic aspects of CNS function, neuroimmunology, metabolism as well as the neurochemistry of neurological and psychiatric disorders of the CNS.
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