THRAP3、STMN1 和 GNA13 在人类血癌细胞中的生物学作用。

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-25 DOI:10.1007/s13205-024-04093-5
Suliman A Alsagaby
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引用次数: 0

摘要

弥漫大 B 细胞淋巴瘤(DLBCL)、伯基特淋巴瘤(BL)和急性髓性白血病(AML)等血癌都是侵袭性肿瘤,其特点是临床过程不理想,生存率低。本次研究的目的是研究 THRAP3、STMN1 和 GNA13 在 DLBCL、BL 和 AML 中的表达,并探讨这些蛋白是否与血癌的预后和进展有关。研究人员使用淋巴捕获器、梯度离心和磁珠分离正常血细胞。流式细胞仪分析表明分离的细胞质量很高。免疫印迹检测发现,THRAP3、STMN1 和 GNA13 在血癌细胞中过表达,但在健康捐献者的正常血细胞中几乎检测不到。同样,利用基因型-组织表达(GTEx)和基因表达谱交互分析(GEPIA)进行的研究表明,与匹配的正常组织相比,这三种蛋白质在各种癌症中的 mRNA 表达量更高(p ≤ 0.01)。此外,这些蛋白的转录表达上调是血癌患者总生存期(OS)缩短的一个特征;p ≤ 0.02。有趣的是,使用 gProfiler 进行的功能分析和使用 STRING 与 cytoscape 进行的蛋白-蛋白相互作用网络分析显示,THRAP3 与癌症依赖性增殖和生存途径相关(校正后 p ≤ 0.05),并与肿瘤发生和化疗耐药性相关蛋白相互作用(p = 1 × 10-16)。综上所述,这些发现表明 THRAP3、STMN1 和 GNA13 可能与血癌的进展和预后有关。要进一步研究和验证本文报告的结果,还需要使用血癌临床样本开展更多工作:在线版本包含补充材料,可在10.1007/s13205-024-04093-5上查阅。
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Biological roles of THRAP3, STMN1 and GNA13 in human blood cancer cells.

Blood cancers, such as diffuse large B-cell lymphoma (DLBCL), Burkitt's lymphoma (BL) and acute myeloid leukemia (AML), are aggressive neoplasms that are characterized by undesired clinical courses with dismal survival rates. The objective of the current work is to study the expression THRAP3, STMN1 and GNA13 in DLBCL, BL and AML, and to investigate if these proteins are implicated in the prognosis and progression of the blood cancers. Isolation of normal blood cells was performed using lymphoprep coupled with gradient centrifugation and magnetic beads. Flow-cytometric analysis showed high quality of the isolated cells. Western blotting identified THRAP3, STMN1 and GNA13 to be overexpressed in the blood cancer cells but hardly detected in normal blood cells from healthy donors. Consistently, investigations performed using genotype-tissue expression (GTEx) and gene expression profiling interactive analysis (GEPIA) showed that the three proteins had higher mRNA expression in various cancers compared with matched normal tissues (p ≤ 0.01). Furthermore, the up-regulated transcript expression of these proteins was a feature of short overall survival (OS; p ≤ 0.02) in patients with the blood cancers. Interestingly, functional profiling using gProfiler and protein-protein interaction network analysis using STRING with cytoscape reported THRAP3 to be associated with cancer-dependent proliferation and survival pathways (corrected p ≤ 0.05) and to interact with proteins (p = 1 × 10-16) implicated in tumourigenesis and chemotherapy resistance. Taken together, these findings indicated a possible implication of THRAP3, STMN1 and GNA13 in the progression and prognosis of the blood cancers. Additional work using clinical samples of the blood cancers is required to further investigate and validate the results reported here.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04093-5.

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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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