用于鉴定脑啡肽-7（MK-7）生产菌株的比色筛选模型。

IF 2.6 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-25 DOI:10.1007/s13205-024-04097-1

Qiu-Hua Zhang, Zheng Wang, Yao-Qiang Wang, Yi-Lin Zhao, Hai-Jia Su

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引用次数: 0

摘要

本研究利用高锰酸钾建立了一种新的比色筛选方法，用于鉴定产生 menaquinone-7 （MK-7）的菌株。据我们所知，这种方法是第一种直接筛选鉴定 MK-7 生产菌株的方法。利用这种筛选方法，我们从青藏高原的土壤中鉴定出了一种新的 MK-7 产菌株--枯草芽孢杆菌 GSA-184。在优化的发酵培养基（50 g/L甘油、30 g/L酵母提取物粉末、100 g/L大豆蛋白胨、1 g/L KH2PO4和1 g/L MnSO4）下，MK-7的产量提高到25.7 mg/L。此外，在 5 升发酵罐中发酵 48 小时后，MK-7 的最大产量达到 36.46 mg/L：在线版本包含补充材料，可查阅 10.1007/s13205-024-04097-1。

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Colorimetric screening model for identification of menaquinone-7 (MK-7) producing strains.

In this study, a novel colorimetric screening method for identifying menaquinone-7 (MK-7) producing strains was established using potassium permanganate. To our knowledge, this method represents the first direct screening methodology for the identification of MK-7 producing strains. Utilizing this screening method, a new MK-7 producing strain, Bacillus subtilis GSA-184, was identified from the soil of the Tibetan Plateau. Under the optimized fermentation medium (50 g/L glycerol, 30 g/L yeast extract powder, 100 g/L soybean peptone, 1 g/L KH₂PO₄, and 1 g/L MnSO₄), the production of MK-7 was increased to 25.7 mg/L. Additionally, the maximum production of MK-7 reached 36.46 mg/L after 48 h in a 5-L fermenter.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04097-1.

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.