椰子犀角金龟子生物控制剂 Oryctes rhinoceros nudivirus 分离物的基因组鉴定。

IF 2.3 3区 生物学 Q3 MICROBIOLOGY Archives of Microbiology Pub Date : 2024-09-26 DOI:10.1007/s00203-024-04116-y
Ela Hiszczynska-Sawicka, Mitchell K. Weston, Aurelie Laugraud, Charles A. Hefer, Jeanne M. E. Jacobs, Sean D. G. Marshall
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引用次数: 0

摘要

椰子犀角金龟(Oryctes rhinoceros,CRB)是椰子和油棕榈的一种严重害虫。它原产于南亚和东南亚,1909 年无意中被引入萨摩亚。上个世纪,它还入侵了许多其他太平洋国家。犀牛裸体病毒(Oryctes rhinoceros nudivirus,OrNV)是 CRB 在其原产地的天然病原体,被成功引入作为经典生物控制剂,几十年来有效抑制了入侵的 CRB 种群。然而,据记录,CRB 又重新出现,在几个太平洋岛屿国家和领土上发现了新的入侵。此外,在一些入侵地区,新的 CRB 种群正在出现,它们对通常用于 CRB 生物防治的病毒分离株具有一定程度的抗性。在此,我们设计了一种快速、可靠的工具来区分不同的 OrNV 病毒分离物,该工具有助于筛选过程,以确定有效的分离物来管理新的 CRB 入侵。通过比较来自原生地和入侵地的 16 个 OrNV 分离物的 OrNV 基因组中的 13 个基因/基因区序列,我们发现了独特的单核苷酸多态性(SNPs)。有了这些 SNPs,我们开发出了一种利用基于多重 PCR-扩增子的纳米孔测序来区分 OrNV 分离物的检测方法。我们发现,在 20 个 OrNV 分离物中,只要有 4 个基因片段就足以鉴定出 15 个。这种方法可作为一种工具,用于监测被选作生物控制剂在 CRB 感染区释放的 OrNV 分离物的建立和分布情况。
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Genomic identification of Oryctes rhinoceros nudivirus isolates, a biocontrol agent for coconut rhinoceros beetle

The coconut rhinoceros beetle (Oryctes rhinoceros, CRB) is a serious pest of coconut and oil palms. It is native to South and Southeast Asia and was inadvertently introduced to Samoa in 1909. It has invaded many other Pacific countries throughout the last century. Oryctes rhinoceros nudivirus (OrNV), a natural pathogen of CRB in its native range, was successfully introduced as a classical biocontrol agent and has effectively suppressed invasive CRB populations for decades. However, resurgence of CRB has been recorded, with new invasions detected in several Pacific Island Countries and Territories. Additionally, new populations of CRB are emerging in some invaded areas that have a degree of resistance to the virus isolates commonly released for CRB biocontrol. Here, we designed a fast and reliable tool for distinguishing between different OrNV isolates that can help with the selection process to identify effective isolates for management of new CRB invasions. A comparison of 13 gene/gene region sequences within the OrNV genome of 16 OrNV isolates from native and invaded ranges allowed us to identify unique Single Nucleotide Polymorphisms (SNPs). With these SNPs, we developed an assay using multiplex PCR-amplicon-based nanopore sequencing to distinguish between OrNV isolates. We found that as few as four gene fragments were sufficient to identify 15 out of 20 OrNV isolates. This method can be used as a tool to monitor the establishment and distribution of OrNV isolates selected for release as biocontrol agents in CRB-infected areas.

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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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