肩周炎中铁蛋白沉积的生物信息学分析

IF 2.1 4区 医学 Q3 GENETICS & HEREDITY BMC Medical Genomics Pub Date : 2024-09-27 DOI:10.1186/s12920-024-02011-5
Hongcui Zhang, Jiahua Zhou, Zhihua Liu, Kaile Wang, Hexun Jiang
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引用次数: 0

摘要

目的:肩周炎是一种常见的肩部疾病,严重影响患者的生活和工作。铁变态反应是一种新型的程序性细胞死亡,与多种疾病有关。然而,目前还没有关于肩周炎与铁中毒之间关系的研究报告。本研究发现了肩周炎中铁突变的潜在分子标记,从而为肩周炎的治疗提供更有效的策略:方法:从基因表达总库(Gene Expression Omnibus,GEO)数据集中下载了GSE238053,并将其与铁沉着基因进行交叉,以获得差异表达基因(DEGs)。通过 WebGestalt 和 Metascape 对 DEGs 的信号通路和生物学功能进行了分析。利用 STRING 和 Cytoscape 分析了这些 DEGs 与肩周炎和铁锈色素沉着症关键基因之间的相互作用。利用肩周炎大鼠模型验证了我们预测的基因,并利用 Western Blot 和 qRT-PCR 评估了相关基因的表达水平:结果:在 GSE238053 和铁蛋白沉积数据库之间共获得了 34 个 DEGs,其中大部分参与了 HIF-1 信号通路和炎症反应。通过 Cytoscape 获得了蛋白-蛋白相互作用网络,并通过 MCODE 筛选出了关键基因(IL-6、HMOX1 和 TLR4)。Western Blot 结果显示,冻肩大鼠模型中 TLR4 和 HMOX1 蛋白表达水平升高,IL-6 蛋白水平下降。肩周炎后的 mRNA 水平显示 IL-6 上调,而 TLR4 和 HMOX1 下调:结论:研究结果表明,铁变态反应可能通过这些信号通路和基因影响肩周炎的病理过程。IL-6、HMOX1 和 TLR4 基因的鉴定可为肩周炎提供新的治疗靶点。
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Bioinformatics analysis of ferroptosis in frozen shoulder.

Objectives: Frozen shoulder is a common shoulder disease that significantly affects the patient's life and work. Ferroptosis is a new type of programmed cell death, which is involved in many diseases. However, there have been no studies reporting the relationship between frozen shoulders and ferroptosis. This study identified potential molecular markers of ferroptosis in frozen shoulders to provide more effective strategies for the treatment of frozen shoulders.

Methods: GSE238053 was downloaded from the Gene Expression Omnibus (GEO) dataset and intersected with ferroptosis genes to obtain differentially expressed genes (DEGs). The signaling pathways and biological functions of DEGs were performed by WebGestalt and Metascape. The interactions related to these DEGs and the key genes between frozen shoulders and ferroptosis was performed by STRING and Cytoscape. A frozen shoulders rat model was used to validate our predicted genes, Western Blot and qRT-PCR was used to assess the expression levels of our genes of interest.

Results: A total of 34 DEGs between GSE238053 and Ferroptosis Database were obtained, most of which were involved in the HIF-1 signaling pathway and inflammatory response. A protein-protein interaction network was obtained by Cytoscape and the key genes (IL-6, HMOX1 and TLR4) were screened by MCODE. Our results of Western Blot showed that the protein expression level of TLR4 and HMOX1 were elevated, and the protein level of IL-6 decreased in frozen shoulders rat model. The mRNA level after frozen shoulders showed that IL-6 was upregulated, whereas TLR4 and HMOX1were downregulated.

Conclusions: The results demonstrated that ferroptosis may affect the pathological process of frozen shoulders through these signaling pathways and genes. The identification of IL-6, HMOX1 and TLR4 genes can provide new therapeutic targets for frozen shoulders.

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来源期刊
BMC Medical Genomics
BMC Medical Genomics 医学-遗传学
CiteScore
3.90
自引率
0.00%
发文量
243
审稿时长
3.5 months
期刊介绍: BMC Medical Genomics is an open access journal publishing original peer-reviewed research articles in all aspects of functional genomics, genome structure, genome-scale population genetics, epigenomics, proteomics, systems analysis, and pharmacogenomics in relation to human health and disease.
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