评估用于挤奶前和挤奶后乳头消毒的 Cremoris 乳球菌制剂。

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES BMC Veterinary Research Pub Date : 2024-09-28 DOI:10.1186/s12917-024-04290-7
A Gazzola, M Zucali, M F Addis, L Bava, S Morandi, S Pisanu, D Pagnozzi, A Passera, M Brasca, R Piccinini
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引用次数: 0

摘要

良好的挤奶卫生习惯包括在挤奶前后对奶牛乳头皮肤进行消毒,目的是防止奶牛发生新的乳房内感染(IMI)。这种做法通常使用消毒剂,而在当前关注抗药性出现的背景下,评估可扩大治疗选择范围的替代解决方案是最令人感兴趣的。与商用消毒剂相比,我们评估了一种基于含细菌素的 cremoris 乳球菌菌株 FT27 培养物的浸泡前和浸泡后产品的功效。之所以选择 FT27,是因为它在体外对食品病原体具有杀菌活性。在本研究中,根据基因组和蛋白质组分析,FT27 对主要的乳腺炎病原体也具有很高的杀菌活性,这很可能与 Nisin A 的产生有关。以乳球菌为基础的制剂应用于意大利北部的两个商业奶牛场。共有 298 头奶牛参加,其中 141 头为治疗组 (TR),157 头为对照组 (CTR)。在 3 个月的时间里,每两周对奶牛进行一次季度采样。试验期间,所有奶牛的健康状况良好。乳房、腹部和腿部的卫生状况普遍良好(平均为
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Assessment of Lactococcus Cremoris preparations for the pre- and post-milking teat disinfection.

Good hygienic milking practices including the disinfection of the cow teat skin before and after milking aimed at preventing the occurrence of new intramammary infections (IMI) in dairy cows. This practice is generally performed using disinfectants, and in the current context of attention to the emergence of resistances, it is of greatest interest to evaluate alternative solutions that can expand treatment options. We assessed the efficacy of a pre-dipping and a post-dipping product based on the bacteriocin-containing culture of Lactococcus cremoris strain FT27, as compared to commercial disinfectants. FT27 was selected for the bactericidal activity in vitro against food pathogens. In the present study, it also revealed a high bactericidal activity against the main mastitis pathogens, most likely related to Nisin A production, according to genomic and proteomic analysis. The lactococcus-based preparations were applied in two commercial dairy farms in Northern Italy. Overall, 298 dairy cows were enrolled, 141 in the treated group (TR), and 157 in the control group (CTR). The cows were sampled at quarter level every two weeks for 3 months. During the trial, all cows showed a good health status. The hygiene level for udder, flanks and legs was generally good (on average < 3 score); the udder hygiene was significantly worse (P < 0.01) in the CTR group. The teat apex hyperkeratosis was overall low (on average < 2 score). We recorded no significant differences between the two experimental groups in the Somatic Cell Count (SCC) values and the bacteriological results. The overall frequency of new IMI was low, ranging 0.6 - 0.5% for S. aureus in the TR or CTR group respectively, to 2.6-4.4% for NASM. Regarding Str. spp., the new IMI accounted for 1.7% or 1.9% in the TR or CTR group, respectively. Notably, the incidence of S. aureus new IMI did not increase during the study, even though neither of the two herds segregated the positive cows. The non-inferiority test showed that the Lactococcus-based pre- and post-dipping products had an efficacy comparable to proven commercial disinfectants in maintaining udder health and preventing new IMI, thus representing a possible alternative to current teat dip products.

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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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