使用 UPLC-Orbitrap-HRMS 分析埃及蜂蜜中的硝基咪唑残留量。

IF 2.5 3区农林科学 Q2 CHEMISTRY, APPLIED Food additives & contaminants. Part B, Surveillance Pub Date : 2024-09-27 DOI:10.1080/19393210.2024.2405998

Dina Rabea, Lamia Ryad, Mohamed R Shehata, Perihan A Khalaf-Alla

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引用次数: 0

摘要

硝基咪唑类是著名的抗菌剂和抗原虫剂，对各种感染有效。然而，它们也可能具有遗传毒性、致癌和诱变作用。本研究旨在利用超高效液相色谱-轨道阱高分辨质谱法（UPLC-Orbitrap-HRMS）开发一种分析方法，用于定量检测蜂蜜中的硝基咪唑类药物残留及其代谢物，并根据欧盟委员会实施条例（EU）2021/808 对该方法进行验证。该方法的检出限（LOD）为 0.01 至 0.17 µg L-1，定量限（LOQ）为 0.020 至 0.29 µg L-1。回收率在 79.8% 到 104% 之间，相对标准偏差在 4.2% 到 19.6% 之间。对 96 份蜂蜜样品的分析表明，18.8% 的样品中存在硝基咪唑残留。这些发现可以更有效地加强埃及对蜂蜜中这些化合物的监测计划，从而提高食品安全。

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Nitroimidazole residues in Egyptian honey using UPLC-Orbitrap-HRMS.

Nitroimidazoles are well-known antibacterial and antiprotozoal agents, effective against various infections. However, they may also exhibit genotoxic, carcinogenic and mutagenic effects. This study aimed to develop an analytical method to quantify nitroimidazole residues and their metabolites in honey using Ultra Performance Liquid Chromatography coupled with Orbitrap High-Resolution Mass Spectrometry (UPLC-Orbitrap-HRMS) and validate it according to Commission Implementing Regulation (EU) 2021/808. The method demonstrated limits of detection (LODs) ranging from 0.01 to 0.17 µg L^-1 and limits of quantification (LOQs) from 0.020 to 0.29 µg L^-1. Recovery rates ranged from 79.8% to 104%, with relative standard deviations (RSDs) between 4.2% and 19.6%. Analysis of 96 honey samples revealed nitroimidazole residues in 18.8% of them. These findings could enhance more effectively the Egyptian monitoring programs for these compounds in honey as to improve food safety.

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来源期刊

Food additives & contaminants. Part B, Surveillance 农林科学-毒理学

CiteScore

5.30

自引率

10.30%

发文量

审稿时长

2.7 months

期刊介绍： Food Additives & Contaminants: Part B publishes surveillance data indicating the presence and levels of occurrence of designated food additives, residues and contaminants in foods, food supplements and animal feed. Data using validated methods must meet stipulated quality standards to be acceptable and must be presented in a prescribed format for subsequent data-handling. Food Additives & Contaminants: Part B restricts its scope to include certain classes of food additives, residues and contaminants. This is based on a goal of covering those areas where there is a need to record surveillance data for the purposes of exposure and risk assessment. The scope is initially restricted to: Additives - food colours, artificial sweeteners, and preservatives; Residues – veterinary drug and pesticide residues; Contaminants – metals, mycotoxins, phycotoxins, plant toxins, nitrate/nitrite, PCDDs/PCFDs, PCBs, PAHs, acrylamide, 3-MPCD and contaminants derived from food packaging. Readership: The readership includes scientists involved in all aspects of food safety and quality and particularly those involved in monitoring human exposure to chemicals from the diet. Papers reporting surveillance data in areas other than the above should be submitted to Part A . The scope of Part B will be expanded from time-to-time to ensure inclusion of new areas of concern.