Corinna Aimee Chavez-Manini, Sandra Alicia Reza-López, Carlos Arzate-Quintana, Celia María Quiñonez-Flores, María Alejandra Favila-Pérez, Javier Camarillo-Cisneros, Alva Rocío Castillo-González
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Quality control criteria considered included specifying control and experimental groups, replicates performed, experimental parameters, and study limitations.</div></div><div><h3>Results</h3><div>Ten studies were included, which involved the strains Xen5, Xen41, PAO1 persistent cells, and PA14. An average reduction of 3.5 log in biofilm formation was observed in the included studies. The electric current parameters that achieved the greatest effect were 500 μA DC with platinum electrodes for 4 days [5.2–5.5 log], 200 μA intermittent with titanium electrodes for 4 days [4.99 log], and 150 ± 60 μA with silver electrodes for 24 h [4 log]. Complete eradication of PAO1 persistent cells was achieved in 1 h with a treatment of 70 μA/cm2 DC followed by 1.5 μg/mL tobramycin for 1 h each.</div></div><div><h3>Conclusions</h3><div>The bactericidal effect of electric current is proportional to the exposure time and current intensity. The electrode material influences the effectiveness of the treatment, possibly because of redox reactions, while differences are observed in the effect on the cell membrane and gene expression when using metallic or carbon electrodes, suggesting differences in the mechanism of action.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"52 ","pages":"Article 100735"},"PeriodicalIF":1.4000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of electric current in viability, biofilm formation and antibiotic resistance of Pseudomonas aeruginosa: A systematic review\",\"authors\":\"Corinna Aimee Chavez-Manini, Sandra Alicia Reza-López, Carlos Arzate-Quintana, Celia María Quiñonez-Flores, María Alejandra Favila-Pérez, Javier Camarillo-Cisneros, Alva Rocío Castillo-González\",\"doi\":\"10.1016/j.ijmmb.2024.100735\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>The bactericidal effect of electric current has been studied in various microorganisms such as <em>Pseudomonas aeruginosa</em>. The objective of this review is to identify the experimental parameters with the greatest antibacterial effect in the shortest time.</div></div><div><h3>Methods</h3><div>Literature search was conducted in the databases PubMed, Science Direct, and Google Scholar. Only original articles published between 2014 and 2023 were included, where the effect of electric current on viability, biofilm formation, and/or antibiotic resistance in <em>P. aeruginosa</em> was analyzed. Quality control criteria considered included specifying control and experimental groups, replicates performed, experimental parameters, and study limitations.</div></div><div><h3>Results</h3><div>Ten studies were included, which involved the strains Xen5, Xen41, PAO1 persistent cells, and PA14. An average reduction of 3.5 log in biofilm formation was observed in the included studies. The electric current parameters that achieved the greatest effect were 500 μA DC with platinum electrodes for 4 days [5.2–5.5 log], 200 μA intermittent with titanium electrodes for 4 days [4.99 log], and 150 ± 60 μA with silver electrodes for 24 h [4 log]. Complete eradication of PAO1 persistent cells was achieved in 1 h with a treatment of 70 μA/cm2 DC followed by 1.5 μg/mL tobramycin for 1 h each.</div></div><div><h3>Conclusions</h3><div>The bactericidal effect of electric current is proportional to the exposure time and current intensity. 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Effect of electric current in viability, biofilm formation and antibiotic resistance of Pseudomonas aeruginosa: A systematic review
Background
The bactericidal effect of electric current has been studied in various microorganisms such as Pseudomonas aeruginosa. The objective of this review is to identify the experimental parameters with the greatest antibacterial effect in the shortest time.
Methods
Literature search was conducted in the databases PubMed, Science Direct, and Google Scholar. Only original articles published between 2014 and 2023 were included, where the effect of electric current on viability, biofilm formation, and/or antibiotic resistance in P. aeruginosa was analyzed. Quality control criteria considered included specifying control and experimental groups, replicates performed, experimental parameters, and study limitations.
Results
Ten studies were included, which involved the strains Xen5, Xen41, PAO1 persistent cells, and PA14. An average reduction of 3.5 log in biofilm formation was observed in the included studies. The electric current parameters that achieved the greatest effect were 500 μA DC with platinum electrodes for 4 days [5.2–5.5 log], 200 μA intermittent with titanium electrodes for 4 days [4.99 log], and 150 ± 60 μA with silver electrodes for 24 h [4 log]. Complete eradication of PAO1 persistent cells was achieved in 1 h with a treatment of 70 μA/cm2 DC followed by 1.5 μg/mL tobramycin for 1 h each.
Conclusions
The bactericidal effect of electric current is proportional to the exposure time and current intensity. The electrode material influences the effectiveness of the treatment, possibly because of redox reactions, while differences are observed in the effect on the cell membrane and gene expression when using metallic or carbon electrodes, suggesting differences in the mechanism of action.
期刊介绍：
Manuscripts of high standard in the form of original research, multicentric studies, meta analysis, are accepted. Current reports can be submitted as brief communications. Case reports must include review of current literature, clinical details, outcome and follow up. Letters to the editor must be a comment on or pertain to a manuscript already published in the IJMM or in relation to preliminary communication of a larger study.
Review articles, Special Articles or Guest Editorials are accepted on invitation.
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