Mahnoor Arif, Syed Basit Rasheed, Habib Ullah, Tawaf Ali Shah, Faiz Ur Rehman, Turki M Dawoud
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DNA was extracted from the blood-fed female <i>A. stephensi</i> mosquitoes using the Ammonium Acetate Precipitation Method followed by PCR analysis, blood meal sources were identified. Nested PCR on 191 pooled samples was used to detect <i>Plasmodium falciparum</i> and <i>Plasmodium vivax</i>.</p><p><strong>Results: </strong>Cattle blood meals were predominant (73%), followed by human (20%) and chicken (7%), with no dog blood meals detected. All individual mosquito samples were negative for <i>Plasmodium falciparum</i>, while two pooled samples (out of 191) tested positive for <i>P. vivax</i>.</p><p><strong>Conclusion: </strong><i>A. stephensi</i> in Khyber District primarily displayed anthropophagic feeding behavior, with a small portion of the population infected with <i>P. vivax</i>. The results underscore the importance of targeted vector control strategies, environmental management, community engagement and continuous monitoring to suppress malaria transmission.</p>","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"19 3","pages":"333-340"},"PeriodicalIF":0.9000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11417983/pdf/","citationCount":"0","resultStr":"{\"title\":\"Feeding Behavior and <i>Plasmodium</i> Detection in <i>Anopheles stephensi</i>, a Malaria Vector in District Khyber, Khyber Pakhtunkhwa, Pakistan.\",\"authors\":\"Mahnoor Arif, Syed Basit Rasheed, Habib Ullah, Tawaf Ali Shah, Faiz Ur Rehman, Turki M Dawoud\",\"doi\":\"10.18502/ijpa.v19i3.16393\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong><i>Anopheles stephensi</i> is a significant malaria vector in Pakistan, and understanding its feeding behavior is necessary to control the spread of malaria. However, limited information is available on the host preferences of <i>A. stephensi</i> in Pakistan. Therefore, we aimed to explore the feeding behavior of <i>A. stephensi</i>, a malaria vector, in the District Khyber, Khyber Pakhtunkhwa, Pakistan.</p><p><strong>Methods: </strong>A total of 7462 mosquitoes were collected between March and September 2021, with 1674 (22.4%) identified as <i>A. stephensi</i> (952 female and 722 male). Among the female <i>A. stephensi</i>, 495 (52%) were blood-fed. DNA was extracted from the blood-fed female <i>A. stephensi</i> mosquitoes using the Ammonium Acetate Precipitation Method followed by PCR analysis, blood meal sources were identified. Nested PCR on 191 pooled samples was used to detect <i>Plasmodium falciparum</i> and <i>Plasmodium vivax</i>.</p><p><strong>Results: </strong>Cattle blood meals were predominant (73%), followed by human (20%) and chicken (7%), with no dog blood meals detected. All individual mosquito samples were negative for <i>Plasmodium falciparum</i>, while two pooled samples (out of 191) tested positive for <i>P. vivax</i>.</p><p><strong>Conclusion: </strong><i>A. stephensi</i> in Khyber District primarily displayed anthropophagic feeding behavior, with a small portion of the population infected with <i>P. vivax</i>. The results underscore the importance of targeted vector control strategies, environmental management, community engagement and continuous monitoring to suppress malaria transmission.</p>\",\"PeriodicalId\":14669,\"journal\":{\"name\":\"Iranian Journal of Parasitology\",\"volume\":\"19 3\",\"pages\":\"333-340\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11417983/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.18502/ijpa.v19i3.16393\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/ijpa.v19i3.16393","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Feeding Behavior and Plasmodium Detection in Anopheles stephensi, a Malaria Vector in District Khyber, Khyber Pakhtunkhwa, Pakistan.
Background: Anopheles stephensi is a significant malaria vector in Pakistan, and understanding its feeding behavior is necessary to control the spread of malaria. However, limited information is available on the host preferences of A. stephensi in Pakistan. Therefore, we aimed to explore the feeding behavior of A. stephensi, a malaria vector, in the District Khyber, Khyber Pakhtunkhwa, Pakistan.
Methods: A total of 7462 mosquitoes were collected between March and September 2021, with 1674 (22.4%) identified as A. stephensi (952 female and 722 male). Among the female A. stephensi, 495 (52%) were blood-fed. DNA was extracted from the blood-fed female A. stephensi mosquitoes using the Ammonium Acetate Precipitation Method followed by PCR analysis, blood meal sources were identified. Nested PCR on 191 pooled samples was used to detect Plasmodium falciparum and Plasmodium vivax.
Results: Cattle blood meals were predominant (73%), followed by human (20%) and chicken (7%), with no dog blood meals detected. All individual mosquito samples were negative for Plasmodium falciparum, while two pooled samples (out of 191) tested positive for P. vivax.
Conclusion: A. stephensi in Khyber District primarily displayed anthropophagic feeding behavior, with a small portion of the population infected with P. vivax. The results underscore the importance of targeted vector control strategies, environmental management, community engagement and continuous monitoring to suppress malaria transmission.
期刊介绍：
Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members.
The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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