用于灵敏荧光检测 2-甲基咪唑的富 P 碳量子点的合成。

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Fluorescence Pub Date : 2024-09-26 DOI:10.1007/s10895-024-03946-9
Zhengyuan Dai, Hao Liu, Xuyuan Sun, Ying Wang, Jie Fan, Li Li, Yaping Ding
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引用次数: 0

摘要

以邻苯二胺为碳源,植酸为磷源,通过改变水热法的反应温度和时间,成功合成了RCD和BCD两种富P碳量子点。实验发现,红色发射的RCDs可实现对2-甲基咪唑的灵敏检测,且2-甲基咪唑对蓝色发射的BCDs无明显的淬灭效应,这使得RCDs成为一种灵敏、快速、选择性好的2-甲基咪唑检测荧光传感器。在最佳实验条件下,RCDs 的荧光强度随 2-甲基咪唑浓度的增加而降低。碳量子点传感器对 2-甲基咪唑浓度的检测在 5 ~ 110 µM 范围内呈现出良好的线性关系,低检测限为 0.61 µM(S/N = 3)。该传感器能够检测湖水中的 2-甲基咪唑,可应用于实际样品。结果表明,这项工作提供了一种检测水中 2-甲基咪唑的简单荧光方法。
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Synthesis of P-Rich Carbon Quantum Dots for Sensitive Fluorescent Detection of 2-Methylimidazole.

Using o-phenylenediamine as carbon source and phytic acid as phosphorus source, two P-rich carbon quantum dots RCDs and BCDs were synthesized successfully by changing the reaction temperature and time of hydrothermal method. It was found that RCDs with red emission could realize sensitive detection of 2-methylimidazole, and 2-methylimidazole had no obvious quenching effect on BCDs with blue emission, which made RCDs a sensitive, quick and selective fluorescence sensor for 2-methylimidazole detection. Under the optimal experimental conditions, the fluorescence intensity of RCDs decreased with the increasing of 2-methylimidazole concentration. The detection of 2-methylimidazole concentration by the carbon quantum dots sensor showed a good linear relationship in the range of 5 ~ 110 µM, and the low detection limit was 0.61 µM (S/N = 3). The sensor is able to detect 2-methylimidazole in lake water, enabling the application of real samples. The results show that this work provides a simple fluorescence method to detect 2-methylimidazole in water.

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来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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