应用微型化最可能数法检测水样中的细菌:检测饮用水中具有多重耐药性的 Ralstonia insidiosa。

IF 2.5 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Journal of water and health Pub Date : 2024-09-01 Epub Date: 2024-08-16 DOI:10.2166/wh.2024.086
Noor Andryan Ilsan, Siti Nurfajriah, Maulin Inggraini, Ni Putu Ratna Ayu Krishanti, Melda Yunita, Reza Anindita, Tzu-Wen Huang
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引用次数: 0

摘要

检测饮用水中的细菌污染对监测食源性疾病的传播至关重要。我们开发了一种简单、便携、低成本的微型最可能数(mini MPN)方法,用于对水中的细菌悬浮液进行半计数,作为饮用水模拟物。在这项研究中,当肺炎克雷伯氏菌悬浮液的菌落形成单位分别为 10 和 100 CFU/ml 时,迷你最可能数与标准方法--技术平板计数(TPC)之间没有明显差异,P 值为 0.28。为了便于使用,我们测试了几个变量,以证明这种方法可以在社会上大规模应用。与无菌标准实验室技术相比,使用无菌塑料移液管、在生物安全柜(BSC)中进行样本接种、使用 3 个月的储存培养基以及在室温下进行培养等变量的 P 值均大于 0.05。在该方法的试验中,我们使用商业化饮用水进行细菌计数和特征描述。我们发现耐多药(MDR)的Ralstonia insidiosa至少对四类抗菌药有耐药性,包括氨基糖苷类、青霉素类、头孢菌素类和碳青霉烯类。Vitek 2 Compact 用于细菌鉴定和抗菌药物敏感性测试。对 Omphisa fuscidentalis 幼虫的毒力测试表明,R. insidiosa 菌株 D 的毒力较低。
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Application of miniaturized most probable number method for bacterial detection in water samples: detection of multi-drug-resistant Ralstonia insidiosa in drinking water.

The detection of bacterial contamination in drinking water is essential for monitoring the spread of foodborne diseases. We developed a simple, portable, and low-cost method of mini most probable number (mini MPN) to semi-enumerate bacterial suspension in water as a drinking water analogue. In this study, there is no significant difference between mini MPN and the standard method, technique plate count (TPC), at 10 and 100 CFU/ml Klebsiella pneumoniae suspension with a P-value of 0.28. For the ease-of-use aspect of this method, we tested several variables to prove it can be mass-applied in society. The usage of a sterile-plastic pipette, sample inoculation conducted in a biosafety cabinet (BSC), the usage of a 3-month storage medium, and incubation temperature conducted at room temperature compared to aseptic standard laboratory technique showed P-value > 0.05. In a trial for this method, we used commercialized drinking water for bacterial enumeration and characterization. We found multi-drug resistant (MDR) Ralstonia insidiosa which was resistant to at least four antimicrobial classes, including aminoglycosides, penicillins, cephalosporin, and carbapenem. Vitek 2 Compact was used for bacterial identification and antimicrobial susceptibility testing. A virulence test in Omphisa fuscidentalis larvae showed R. insidiosa strain D had a low virulence.

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来源期刊
Journal of water and health
Journal of water and health 环境科学-环境科学
CiteScore
3.60
自引率
8.70%
发文量
110
审稿时长
18-36 weeks
期刊介绍: Journal of Water and Health is a peer-reviewed journal devoted to the dissemination of information on the health implications and control of waterborne microorganisms and chemical substances in the broadest sense for developing and developed countries worldwide. This is to include microbial toxins, chemical quality and the aesthetic qualities of water.
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