Islamudin Ahmad, Lizma Febrina, Baso Didik Hikmawan, Junaidin Junaidin, Herman Herman, Muhammad Faisal, Erwin Samsul, Iswahyudi Iswahyudi, Arsyik Ibrahim, Reza Yuridian Purwoko, Tia Okselni, Marissa Angelina, Amal Rezka Putra, Ratna Surya Alwi, Abdul Mun'im
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引用次数: 0
摘要
采用一维核磁共振与二维核磁共振相结合的方法评估了榄香脂球藻乙醇提取物的代谢物概况。球茎榄香脂是印度尼西亚的一种本土植物,有望成为药用产品的原料。我们使用 DPPH 和 ABTS 方法检测了 E. bulbosa 提取物的抗氧化能力。抗氧化评估结果表明,乙醇提取物具有很强的抗氧化性。此外,我们还成功鉴定了乙醇提取物中的 13 种代谢物,这些代谢物未经分离。这些代谢物包括:榄香脂醇 A、榄香脂醇 B、榄香脂醇 C、榄香硫酮 B、榄香硫酮 C、红花苷、卡瓦胡椒酚、异榄香脂苷 C、奎宁酸、绿原酸、异榄香脂苷、花青素-3-O-β-吡喃葡萄糖苷和卡德酸。代谢物花青素-3-O-β-吡喃葡萄糖苷(4.89 mM ± 0.02)是该提取物中含量最高的代谢物。这一结果表明,核磁共振方法无需分离即可检测和测量球茎草乙醇提取物中的代谢物。
NMR-based metabolic profiling and antioxidant activity of Eleutherine bulbosa bulb ethanol extract.
The metabolite profile of the ethanol extract from Eleutherine bulbosa was assessed using the 1D NMR approach amalgamated with 2D NMR. E. bulbosa, an indigenous plant found in Indonesia, possesses considerable promise as a raw material for medicinal products. We examined the antioxidant capacity of the E. bulbosa extract using DPPH and ABTS methods. The antioxidant evaluation demonstrated that the ethanol extract exhibits strong antioxidant properties. Furthermore, we have successfully identified thirteen metabolites in the ethanol extract without separation. These metabolites include eleutherol A, eleutherol B, eleutherol C, eleuthione B, eleuthione C, hongconin, karwinaphthol, isoeleuthoside C, quinic acid, chlorogenic acid, isoeleutherin, cyanidin-3-o-β-glucopyranoside, and kadsuric acid. Metabolite Cyanidin-3-o-β-glucopyranoside (4.89 mM ± 0.02) was the highest metabolite contained in this extract. This result showed that NMR methods could detect and measure metabolites in the E. bulbosa ethanol extract without requiring separation.
期刊介绍:
The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds.
The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal.
Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section.
All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.