{"title":"包虫病患者血浆中微小核糖核酸表达谱的试验性研究:包虫病的潜在免疫调节作用。","authors":"Jing Xiao, Jian-Wen Wu, Yun-Zhuo Xin, Dong Song, Xiao-Ping Gao, Mei Yin, Wei Zhao, Fu-Lin Liu, Hao Wang, Jie Wang, Jia-Qing Zhao","doi":"10.1007/s00436-024-08350-7","DOIUrl":null,"url":null,"abstract":"<p><p>Echinococcosis is a zoonotic disease, which seriously endangers human health. The immune game between parasite and host is not fully understood. Exosomes are thought to be one of the ways of information communication between parasite and host. In this study, we attempted to explore the communication between Echinococcus granulosus and its host through the medium of exosomes. We collected plasma from E. granulosus patients (CE-EXO) and healthy donors (HD-EXO) and extracted exosomes from the plasma. The expression profile of miRNA in plasma was determined by second generation sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to annotate the function of target genes of differential miRNAs. Meanwhile, we co-cultured plasma exosomes from healthy donors and plasma exosomes from E. granulosus patients with Jurkat T cells with or without phytohaemagglutinin (PHA) stimulation. The expression of CD69 on Jurkat T cells was detected by flow cytometry. The results showed that the miRNA of exosomes between healthy donors and E. granulosus patients was significantly different. GO and KEGG were used to annotate the function of target genes of differential miRNAs. The results indicate that many important pathways are involved in inflammation, metabolism, and immune response after parasite infection, such as p53 signaling pathway, PI3K-Akt signaling pathway, and glycolysis/gluconeogenesis. Flow cytometry showed that CE-EXO reduced the expression of CD69 + on Jurkat T cells. Our present results suggest that these differentially expressed miRNAs may be important regulators of parasite-host interactions. Meanwhile, functional prediction of its target genes provides valuable information for understanding the mechanism of host-parasite interactions. These results provide clues for future studies on E. granulosus escape from host immune attack, which could help control E. granulosus infection.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A pilot study of microRNAs expression profile in plasma of patients with hydatid disease: potential immunomodulation of hydatid disease.\",\"authors\":\"Jing Xiao, Jian-Wen Wu, Yun-Zhuo Xin, Dong Song, Xiao-Ping Gao, Mei Yin, Wei Zhao, Fu-Lin Liu, Hao Wang, Jie Wang, Jia-Qing Zhao\",\"doi\":\"10.1007/s00436-024-08350-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Echinococcosis is a zoonotic disease, which seriously endangers human health. The immune game between parasite and host is not fully understood. Exosomes are thought to be one of the ways of information communication between parasite and host. In this study, we attempted to explore the communication between Echinococcus granulosus and its host through the medium of exosomes. We collected plasma from E. granulosus patients (CE-EXO) and healthy donors (HD-EXO) and extracted exosomes from the plasma. The expression profile of miRNA in plasma was determined by second generation sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to annotate the function of target genes of differential miRNAs. Meanwhile, we co-cultured plasma exosomes from healthy donors and plasma exosomes from E. granulosus patients with Jurkat T cells with or without phytohaemagglutinin (PHA) stimulation. The expression of CD69 on Jurkat T cells was detected by flow cytometry. The results showed that the miRNA of exosomes between healthy donors and E. granulosus patients was significantly different. GO and KEGG were used to annotate the function of target genes of differential miRNAs. The results indicate that many important pathways are involved in inflammation, metabolism, and immune response after parasite infection, such as p53 signaling pathway, PI3K-Akt signaling pathway, and glycolysis/gluconeogenesis. Flow cytometry showed that CE-EXO reduced the expression of CD69 + on Jurkat T cells. Our present results suggest that these differentially expressed miRNAs may be important regulators of parasite-host interactions. Meanwhile, functional prediction of its target genes provides valuable information for understanding the mechanism of host-parasite interactions. These results provide clues for future studies on E. granulosus escape from host immune attack, which could help control E. granulosus infection.</p>\",\"PeriodicalId\":19968,\"journal\":{\"name\":\"Parasitology Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Parasitology Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s00436-024-08350-7\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasitology Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00436-024-08350-7","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
棘球蚴病是一种人畜共患病,严重危害人类健康。寄生虫与宿主之间的免疫博弈尚未完全明了。外泌体被认为是寄生虫与宿主之间进行信息交流的途径之一。在这项研究中,我们试图通过外泌体这一媒介来探索粒棘球蚴与宿主之间的交流。我们采集了粒棘球蚴患者(CE-EXO)和健康供体(HD-EXO)的血浆,并从血浆中提取了外泌体。通过二代测序测定了血浆中 miRNA 的表达谱。基因本体(GO)和京都基因组百科全书(KEGG)被用来注释不同 miRNA 的靶基因功能。同时,我们将健康供体的血浆外泌体和粒细胞埃希氏菌患者的血浆外泌体与Jurkat T细胞在植物血凝素(PHA)刺激或无植物血凝素(PHA)刺激下进行了共培养。流式细胞术检测了 Jurkat T 细胞上 CD69 的表达。结果表明,健康供体和肉芽肿埃希氏菌患者外泌体的 miRNA 有显著差异。研究人员利用 GO 和 KEGG 对不同 miRNA 的靶基因功能进行了注释。结果表明,寄生虫感染后,许多重要通路都参与了炎症、新陈代谢和免疫反应,如p53信号通路、PI3K-Akt信号通路和糖酵解/糖元生成。流式细胞术显示,CE-EXO 降低了 Jurkat T 细胞上 CD69 + 的表达。我们目前的研究结果表明,这些不同表达的 miRNA 可能是寄生虫与宿主相互作用的重要调节因子。同时,对其靶基因的功能预测为了解宿主-寄生虫相互作用的机制提供了有价值的信息。这些结果为今后研究肉毒梭菌逃避宿主免疫攻击提供了线索,有助于控制肉毒梭菌感染。
A pilot study of microRNAs expression profile in plasma of patients with hydatid disease: potential immunomodulation of hydatid disease.
Echinococcosis is a zoonotic disease, which seriously endangers human health. The immune game between parasite and host is not fully understood. Exosomes are thought to be one of the ways of information communication between parasite and host. In this study, we attempted to explore the communication between Echinococcus granulosus and its host through the medium of exosomes. We collected plasma from E. granulosus patients (CE-EXO) and healthy donors (HD-EXO) and extracted exosomes from the plasma. The expression profile of miRNA in plasma was determined by second generation sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to annotate the function of target genes of differential miRNAs. Meanwhile, we co-cultured plasma exosomes from healthy donors and plasma exosomes from E. granulosus patients with Jurkat T cells with or without phytohaemagglutinin (PHA) stimulation. The expression of CD69 on Jurkat T cells was detected by flow cytometry. The results showed that the miRNA of exosomes between healthy donors and E. granulosus patients was significantly different. GO and KEGG were used to annotate the function of target genes of differential miRNAs. The results indicate that many important pathways are involved in inflammation, metabolism, and immune response after parasite infection, such as p53 signaling pathway, PI3K-Akt signaling pathway, and glycolysis/gluconeogenesis. Flow cytometry showed that CE-EXO reduced the expression of CD69 + on Jurkat T cells. Our present results suggest that these differentially expressed miRNAs may be important regulators of parasite-host interactions. Meanwhile, functional prediction of its target genes provides valuable information for understanding the mechanism of host-parasite interactions. These results provide clues for future studies on E. granulosus escape from host immune attack, which could help control E. granulosus infection.
期刊介绍:
The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite.
Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology;
Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.