Wenwen Yu , Hua Ye , Yunlei Li , Xiaoqiong Bao, Yangyang Ni, Xiangxiang Chen, Yangjie Sun, Ali Chen, Weilong Zhou, Jifa Li
{"title":"卡氏肺囊虫感染导致 Fn1 表达上调，从而引起肺纤维化和炎症反应。","authors":"Wenwen Yu , Hua Ye , Yunlei Li , Xiaoqiong Bao, Yangyang Ni, Xiangxiang Chen, Yangjie Sun, Ali Chen, Weilong Zhou, Jifa Li","doi":"10.1016/j.riam.2024.04.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div><em>Pneumocystis carinii</em> is an opportunistic fungal pathogen that may cause pneumonia and lead to pulmonary fibrosis.</div></div><div><h3>Aims</h3><div>This study attempted to investigate the role of <em>P. carinii</em> infection-related genes in regulating lung fibrosis in mice.</div></div><div><h3>Methods</h3><div>A screening of <em>P. carinii</em> infection-related differential mRNAs was performed using the GEO database, followed by protein–protein interaction (PPI) network construction using the STRING website in order to obtain <em>P. carinii</em> infection-related key genes. The development of a mouse model with gene aberrant expression was achieved by utilizing mice carrying the Cre-LoxP recombinase system. Dexamethasone was employed to induce tracheal infection in order to develop a model of pulmonary fibrosis, and the magnitude of lung injury was assessed by performing hematoxylin–eosin (H&E) staining and Masson staining. Lung coefficient and hydroxyproline level were assessed on sections of lung tissue as well. Finally, the magnitude of lung fibrosis and inflammation in mice was determined based on immunofluorescence and on the expression of genes associated with lung fibrosis and inflammation.</div></div><div><h3>Results</h3><div>Fn1 was found by PPI with the highest connectivity in the PPI network associated with immunity and inflammation. Besides, Fn1 was significantly highly expressed in <em>P. carinii</em>-infected mice samples. The <em>P carinii</em> pneumonia (PCP)+Fn1<sup>fl/fl</sup> group had significantly higher lung coefficients, hydroxyproline levels and TNF-α, IL-6, IL-1β, IL-8 and NLRP3 expression levels, and significantly lower IL-10 expression levels. The results found in PCP+SPC-Cre:Fn1<sup>fl/fl</sup> group were the opposite. The results of the pulmonary fibrosis level study showed that the PCP+Fn1<sup>fl/fl</sup> group had the most intense H&E and Masson staining, and significantly higher expression levels of Col1A2, Col3A1 and α-SMA, which were lower in the PCP+SPC-Cre:Fn1<sup>fl/fl</sup> group.</div></div><div><h3>Conclusions</h3><div><em>P. carinii</em> infection may promote the upregulation of Fn1, which causes pulmonary fibrosis with an inflammatory response.</div></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":"41 1","pages":"Pages 17-26"},"PeriodicalIF":1.5000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pneumocystis carinii infection drives upregulation of Fn1 expression that causes pulmonary fibrosis with an inflammatory response\",\"authors\":\"Wenwen Yu , Hua Ye , Yunlei Li , Xiaoqiong Bao, Yangyang Ni, Xiangxiang Chen, Yangjie Sun, Ali Chen, Weilong Zhou, Jifa Li\",\"doi\":\"10.1016/j.riam.2024.04.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div><em>Pneumocystis carinii</em> is an opportunistic fungal pathogen that may cause pneumonia and lead to pulmonary fibrosis.</div></div><div><h3>Aims</h3><div>This study attempted to investigate the role of <em>P. carinii</em> infection-related genes in regulating lung fibrosis in mice.</div></div><div><h3>Methods</h3><div>A screening of <em>P. carinii</em> infection-related differential mRNAs was performed using the GEO database, followed by protein–protein interaction (PPI) network construction using the STRING website in order to obtain <em>P. carinii</em> infection-related key genes. The development of a mouse model with gene aberrant expression was achieved by utilizing mice carrying the Cre-LoxP recombinase system. Dexamethasone was employed to induce tracheal infection in order to develop a model of pulmonary fibrosis, and the magnitude of lung injury was assessed by performing hematoxylin–eosin (H&E) staining and Masson staining. Lung coefficient and hydroxyproline level were assessed on sections of lung tissue as well. Finally, the magnitude of lung fibrosis and inflammation in mice was determined based on immunofluorescence and on the expression of genes associated with lung fibrosis and inflammation.</div></div><div><h3>Results</h3><div>Fn1 was found by PPI with the highest connectivity in the PPI network associated with immunity and inflammation. Besides, Fn1 was significantly highly expressed in <em>P. carinii</em>-infected mice samples. The <em>P carinii</em> pneumonia (PCP)+Fn1<sup>fl/fl</sup> group had significantly higher lung coefficients, hydroxyproline levels and TNF-α, IL-6, IL-1β, IL-8 and NLRP3 expression levels, and significantly lower IL-10 expression levels. The results found in PCP+SPC-Cre:Fn1<sup>fl/fl</sup> group were the opposite. The results of the pulmonary fibrosis level study showed that the PCP+Fn1<sup>fl/fl</sup> group had the most intense H&E and Masson staining, and significantly higher expression levels of Col1A2, Col3A1 and α-SMA, which were lower in the PCP+SPC-Cre:Fn1<sup>fl/fl</sup> group.</div></div><div><h3>Conclusions</h3><div><em>P. carinii</em> infection may promote the upregulation of Fn1, which causes pulmonary fibrosis with an inflammatory response.</div></div>\",\"PeriodicalId\":21291,\"journal\":{\"name\":\"Revista Iberoamericana De Micologia\",\"volume\":\"41 1\",\"pages\":\"Pages 17-26\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Iberoamericana De Micologia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1130140624000068\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Iberoamericana De Micologia","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1130140624000068","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
Pneumocystis carinii infection drives upregulation of Fn1 expression that causes pulmonary fibrosis with an inflammatory response
Background
Pneumocystis carinii is an opportunistic fungal pathogen that may cause pneumonia and lead to pulmonary fibrosis.
Aims
This study attempted to investigate the role of P. carinii infection-related genes in regulating lung fibrosis in mice.
Methods
A screening of P. carinii infection-related differential mRNAs was performed using the GEO database, followed by protein–protein interaction (PPI) network construction using the STRING website in order to obtain P. carinii infection-related key genes. The development of a mouse model with gene aberrant expression was achieved by utilizing mice carrying the Cre-LoxP recombinase system. Dexamethasone was employed to induce tracheal infection in order to develop a model of pulmonary fibrosis, and the magnitude of lung injury was assessed by performing hematoxylin–eosin (H&E) staining and Masson staining. Lung coefficient and hydroxyproline level were assessed on sections of lung tissue as well. Finally, the magnitude of lung fibrosis and inflammation in mice was determined based on immunofluorescence and on the expression of genes associated with lung fibrosis and inflammation.
Results
Fn1 was found by PPI with the highest connectivity in the PPI network associated with immunity and inflammation. Besides, Fn1 was significantly highly expressed in P. carinii-infected mice samples. The P carinii pneumonia (PCP)+Fn1fl/fl group had significantly higher lung coefficients, hydroxyproline levels and TNF-α, IL-6, IL-1β, IL-8 and NLRP3 expression levels, and significantly lower IL-10 expression levels. The results found in PCP+SPC-Cre:Fn1fl/fl group were the opposite. The results of the pulmonary fibrosis level study showed that the PCP+Fn1fl/fl group had the most intense H&E and Masson staining, and significantly higher expression levels of Col1A2, Col3A1 and α-SMA, which were lower in the PCP+SPC-Cre:Fn1fl/fl group.
Conclusions
P. carinii infection may promote the upregulation of Fn1, which causes pulmonary fibrosis with an inflammatory response.
期刊介绍：
Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.
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