通过全基因组关联图谱破解栽培和野生大豆品种耐盐性的遗传基础。

IF 4.4 1区 农林科学 Q1 AGRONOMY Theoretical and Applied Genetics Pub Date : 2024-09-28 DOI:10.1007/s00122-024-04752-4
Rajat Pruthi, Chanderkant Chaudhary, Sandeep Chapagain, Mostafa Mohamed Elbasuoni Abozaid, Prabhat Rana, Ravi Kiran Reddy Kondi, Roberto Fritsche-Neto, Prasanta K Subudhi
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引用次数: 0

摘要

关键信息:在一项涉及 269 个栽培和野生大豆品种的全基因组关联研究中,发现了潜在的耐盐性供体以及重要的标记和候选基因,如 GmKUP6 和 GmWRKY33。盐胁迫仍然是农业系统面临的一个重大挑战,尤其影响着全球大豆的产量。为了阐明大豆耐盐性的遗传基础并确定大豆基因型耐盐性的新来源,我们开展了一项全面的全基因组关联研究(GWAS)。在受控温室条件下,对由 269 个野生和栽培大豆品系组成的多样性面板进行了盐胁迫试验。表型数据显示,大豆种质资源的耐盐性受到钠和氯积累的严重影响,叶片焦枯评分与叶片钠/氯含量呈高度显著的正相关。利用 32,832 个 SNP 数据集进行的 GWAS 分析揭示了与耐盐性相关的七个性状中的 32 个显著标记-性状关联(MTAs)。这些标记解释了很大一部分表型变异,从 14% 到 52% 不等。值得注意的是,有 11 个标记超过了 Bonferroni 校正阈值,显示出与相应性状的高度显著关联。在已确定的 MTA 的 100 Kb 范围内进行的基因本体富集分析突出显示了候选基因,如钾转运体 6(GmKUP6)、阳离子氢交换器(GmCHX15)和 GmWRKY33。在盐胁迫条件下,GmKUP6 和 GmWRKY33 的表达水平在耐盐大豆和感盐大豆之间存在显著差异。本研究发现的遗传标记和候选基因有望培育出耐盐胁迫的大豆品种,从而减轻盐胁迫的不利影响。
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Deciphering the genetic basis of salinity tolerance in a diverse panel of cultivated and wild soybean accessions by genome-wide association mapping.

Key message: In a genome-wide association study involving 269 cultivated and wild soybean accessions, potential salt tolerance donors were identified along with significant markers and candidate genes, such as GmKUP6 and GmWRKY33. Salt stress remains a significant challenge in agricultural systems, notably impacting soybean productivity worldwide. A comprehensive genome-wide association study (GWAS) was conducted to elucidate the genetic underpinnings of salt tolerance and identify novel source of salt tolerance among soybean genotypes. A diverse panel comprising 269 wild and cultivated soybean accessions was subjected to saline stress under controlled greenhouse conditions. Phenotypic data revealed that salt tolerance of soybean germplasm accessions was heavily compromised by the accumulation of sodium and chloride, as indicated by highly significant positive correlations of leaf scorching score with leaf sodium/chloride content. The GWAS analysis, leveraging a dataset of 32,832 SNPs, unveiled 32 significant marker-trait associations (MTAs) across seven traits associated with salt tolerance. These markers explained a substantial portion of the phenotypic variation, ranging from 14 to 52%. Notably, 11 markers surpassed Bonferroni's correction threshold, exhibiting highly significant associations with the respective traits. Gene Ontology enrichment analysis conducted within a 100 Kb range of the identified MTAs highlighted candidate genes such as potassium transporter 6 (GmKUP6), cation hydrogen exchanger (GmCHX15), and GmWRKY33. Expression levels of GmKUP6 and GmWRKY33 significantly varied between salt-tolerant and salt-susceptible soybean accessions under salt stress. The genetic markers and candidate genes identified in this study hold promise for developing soybean varieties resilient to salinity stress, thereby mitigating its adverse effects.

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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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