分子基因分型发现菲律宾一家三级医院临床分离的鲍曼不动杆菌中存在多种碳青霉烯酶基因和独特的 blaOXA-51-like (oxaAb) 等位基因。

IF 3.6 Q1 TROPICAL MEDICINE Tropical Medicine and Health Pub Date : 2024-09-26 DOI:10.1186/s41182-024-00629-w
Mark B Carascal, Raul V Destura, Windell L Rivera
{"title":"分子基因分型发现菲律宾一家三级医院临床分离的鲍曼不动杆菌中存在多种碳青霉烯酶基因和独特的 blaOXA-51-like (oxaAb) 等位基因。","authors":"Mark B Carascal, Raul V Destura, Windell L Rivera","doi":"10.1186/s41182-024-00629-w","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Acinetobacter baumannii continued to be an important Gram-negative pathogen of concern in the clinical context. The resistance of this pathogen to carbapenems due to the production of carbapenemases is considered a global threat. Despite the efforts to track carbapenemase synthesis among A. baumannii in the Philippines, local data on its molecular features are very scarce. This study aims to characterize A. baumannii clinical isolates from a Philippine tertiary hospital through genotyping of the pathogen's carbapenemase genes.</p><p><strong>Methods: </strong>Antibiotic susceptibility profiling, phenotypic testing of carbapenemase production, and polymerase chain reaction assays to detect the different classes of carbapenemase genes (class A bla<sub>KPC</sub>, class B bla<sub>NDM</sub>, bla<sub>IMP</sub>, bla<sub>VIM</sub>, and class D bla<sub>OXA-23-like</sub>, bla<sub>OXA-24/40-like</sub>, bla<sub>OXA-48-like</sub>, bla<sub>OXA-51-like</sub>, ISAba1-bla<sub>OXA-51-like</sub>, bla<sub>OXA-58-like</sub>) were performed in all collected A. baumannii, both carbapenem resistant and susceptible (n = 52).</p><p><strong>Results: </strong>Results showed that the majority of the carbapenem-resistant strains phenotypically produced carbapenemases (up to 84% in carbapenem inactivation methods) and possessed the ISAba1-bla<sub>OXA-51-like</sub> gene complex (80%). Meanwhile, both carbapenem-resistant and carbapenem-susceptible isolates possessed multi-class carbapenemase genes including bla<sub>NDM</sub> (1.9%), bla<sub>VIM</sub> (3.9%), bla<sub>OXA-24/40-like</sub> (5.8%), bla<sub>OXA-58-like</sub> (5.8%), bla<sub>KPC</sub> (11.5%), and bla<sub>OXA-23-like</sub> (94.2%), which coexist with each other in some strains (17.3%). In terms of the intrinsic bla<sub>OXA-51-like</sub> (oxaAb) genes, 23 unique alleles were reported (bla<sub>OXA-1058</sub> to bla<sub>OXA-1080</sub>), the majority of which are closely related to bla<sub>OXA-66</sub>. Isolates possessing these alleles showed varying carbapenem resistance profiles.</p><p><strong>Conclusions: </strong>In summary, this study highlighted the importance of molecular genotyping in the characterization of A. baumannii by revealing the carbapenemase profiles of the pathogen (which may not be captured accurately in phenotypic tests), in identifying potent carriers of transferrable carbapenemase genes (which may not be expressed straightforwardly in antimicrobial susceptibility testing), and in monitoring unique pathogen epidemiology in the local clinical setting.</p>","PeriodicalId":23311,"journal":{"name":"Tropical Medicine and Health","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11426070/pdf/","citationCount":"0","resultStr":"{\"title\":\"Molecular genotyping reveals multiple carbapenemase genes and unique bla<sub>OXA-51-like</sub> (oxaAb) alleles among clinically isolated Acinetobacter baumannii from a Philippine tertiary hospital.\",\"authors\":\"Mark B Carascal, Raul V Destura, Windell L Rivera\",\"doi\":\"10.1186/s41182-024-00629-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Acinetobacter baumannii continued to be an important Gram-negative pathogen of concern in the clinical context. The resistance of this pathogen to carbapenems due to the production of carbapenemases is considered a global threat. Despite the efforts to track carbapenemase synthesis among A. baumannii in the Philippines, local data on its molecular features are very scarce. This study aims to characterize A. baumannii clinical isolates from a Philippine tertiary hospital through genotyping of the pathogen's carbapenemase genes.</p><p><strong>Methods: </strong>Antibiotic susceptibility profiling, phenotypic testing of carbapenemase production, and polymerase chain reaction assays to detect the different classes of carbapenemase genes (class A bla<sub>KPC</sub>, class B bla<sub>NDM</sub>, bla<sub>IMP</sub>, bla<sub>VIM</sub>, and class D bla<sub>OXA-23-like</sub>, bla<sub>OXA-24/40-like</sub>, bla<sub>OXA-48-like</sub>, bla<sub>OXA-51-like</sub>, ISAba1-bla<sub>OXA-51-like</sub>, bla<sub>OXA-58-like</sub>) were performed in all collected A. baumannii, both carbapenem resistant and susceptible (n = 52).</p><p><strong>Results: </strong>Results showed that the majority of the carbapenem-resistant strains phenotypically produced carbapenemases (up to 84% in carbapenem inactivation methods) and possessed the ISAba1-bla<sub>OXA-51-like</sub> gene complex (80%). Meanwhile, both carbapenem-resistant and carbapenem-susceptible isolates possessed multi-class carbapenemase genes including bla<sub>NDM</sub> (1.9%), bla<sub>VIM</sub> (3.9%), bla<sub>OXA-24/40-like</sub> (5.8%), bla<sub>OXA-58-like</sub> (5.8%), bla<sub>KPC</sub> (11.5%), and bla<sub>OXA-23-like</sub> (94.2%), which coexist with each other in some strains (17.3%). In terms of the intrinsic bla<sub>OXA-51-like</sub> (oxaAb) genes, 23 unique alleles were reported (bla<sub>OXA-1058</sub> to bla<sub>OXA-1080</sub>), the majority of which are closely related to bla<sub>OXA-66</sub>. Isolates possessing these alleles showed varying carbapenem resistance profiles.</p><p><strong>Conclusions: </strong>In summary, this study highlighted the importance of molecular genotyping in the characterization of A. baumannii by revealing the carbapenemase profiles of the pathogen (which may not be captured accurately in phenotypic tests), in identifying potent carriers of transferrable carbapenemase genes (which may not be expressed straightforwardly in antimicrobial susceptibility testing), and in monitoring unique pathogen epidemiology in the local clinical setting.</p>\",\"PeriodicalId\":23311,\"journal\":{\"name\":\"Tropical Medicine and Health\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11426070/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical Medicine and Health\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s41182-024-00629-w\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"TROPICAL MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Medicine and Health","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s41182-024-00629-w","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"TROPICAL MEDICINE","Score":null,"Total":0}
引用次数: 0

摘要

背景:鲍曼不动杆菌(Acinetobacter baumannii)仍然是临床上令人担忧的重要革兰氏阴性病原体。这种病原体因产生碳青霉烯酶而对碳青霉烯类产生耐药性,被认为是一种全球性威胁。尽管菲律宾在努力追踪鲍曼不动杆菌中碳青霉烯酶的合成情况,但有关其分子特征的本地数据却非常稀少。本研究旨在通过对病原体的碳青霉烯酶基因进行基因分型,分析菲律宾一家三甲医院的鲍曼不动杆菌临床分离株的特征:方法:对收集到的所有鲍曼不动杆菌临床分离株进行抗生素敏感性分析、碳青霉烯酶生产表型检测和聚合酶链反应检测,以检测不同类别的碳青霉烯酶基因(A 类 blaKPC、B 类 blaNDM、blaIMP、blaVIM 和 D 类 blaOXA-23-like、blaOXA-24/40-like、blaOXA-48-like、blaOXA-51-like、ISAba1-blaOXA-51-like、blaOXA-58-like)。结果:结果表明,大多数耐碳青霉烯菌株表型上产生碳青霉烯酶(在碳青霉烯灭活方法中高达 84%),并拥有 ISAba1-blaOXA-51-like 基因复合物(80%)。同时,耐碳青霉烯类和对碳青霉烯类敏感的分离株都具有多类碳青霉烯酶基因,包括 blaNDM(1.9%)、blaVIM(3.9%)、blaOXA-24/40-like(5.8%)、blaOXA-58-like(5.8%)、blaKPC(11.5%)和 blaOXA-23-like(94.2%),这些基因在一些菌株(17.3%)中相互共存。就内在 blaOXA-51-like(oxaAb)基因而言,报告了 23 个独特的等位基因(blaOXA-1058 至 blaOXA-1080),其中大多数与 blaOXA-66 关系密切。具有这些等位基因的分离株显示出不同的碳青霉烯耐药性特征:总之,本研究通过揭示病原体的碳青霉烯酶谱(表型检测可能无法准确捕捉)、识别可转移碳青霉烯酶基因的强力携带者(抗菌药敏感性检测可能无法直接表达)以及监测当地临床环境中独特的病原体流行病学,强调了分子基因分型在鲍曼尼菌特征描述中的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Molecular genotyping reveals multiple carbapenemase genes and unique blaOXA-51-like (oxaAb) alleles among clinically isolated Acinetobacter baumannii from a Philippine tertiary hospital.

Background: Acinetobacter baumannii continued to be an important Gram-negative pathogen of concern in the clinical context. The resistance of this pathogen to carbapenems due to the production of carbapenemases is considered a global threat. Despite the efforts to track carbapenemase synthesis among A. baumannii in the Philippines, local data on its molecular features are very scarce. This study aims to characterize A. baumannii clinical isolates from a Philippine tertiary hospital through genotyping of the pathogen's carbapenemase genes.

Methods: Antibiotic susceptibility profiling, phenotypic testing of carbapenemase production, and polymerase chain reaction assays to detect the different classes of carbapenemase genes (class A blaKPC, class B blaNDM, blaIMP, blaVIM, and class D blaOXA-23-like, blaOXA-24/40-like, blaOXA-48-like, blaOXA-51-like, ISAba1-blaOXA-51-like, blaOXA-58-like) were performed in all collected A. baumannii, both carbapenem resistant and susceptible (n = 52).

Results: Results showed that the majority of the carbapenem-resistant strains phenotypically produced carbapenemases (up to 84% in carbapenem inactivation methods) and possessed the ISAba1-blaOXA-51-like gene complex (80%). Meanwhile, both carbapenem-resistant and carbapenem-susceptible isolates possessed multi-class carbapenemase genes including blaNDM (1.9%), blaVIM (3.9%), blaOXA-24/40-like (5.8%), blaOXA-58-like (5.8%), blaKPC (11.5%), and blaOXA-23-like (94.2%), which coexist with each other in some strains (17.3%). In terms of the intrinsic blaOXA-51-like (oxaAb) genes, 23 unique alleles were reported (blaOXA-1058 to blaOXA-1080), the majority of which are closely related to blaOXA-66. Isolates possessing these alleles showed varying carbapenem resistance profiles.

Conclusions: In summary, this study highlighted the importance of molecular genotyping in the characterization of A. baumannii by revealing the carbapenemase profiles of the pathogen (which may not be captured accurately in phenotypic tests), in identifying potent carriers of transferrable carbapenemase genes (which may not be expressed straightforwardly in antimicrobial susceptibility testing), and in monitoring unique pathogen epidemiology in the local clinical setting.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Tropical Medicine and Health
Tropical Medicine and Health TROPICAL MEDICINE-
CiteScore
7.00
自引率
2.20%
发文量
90
审稿时长
11 weeks
期刊最新文献
Trends and inequalities in the use of deworming medication during pregnancy in Sierra Leone, 2008-2019. Comments on: prevalence and risk factors for tuberculosis and HIV coinfections in Kenyan prison inmates. Comment on: Fetal femur length and risk of diabetes in adolescence: a prospective cohort study. Reply to: Comment on: Fetal femur length and risk of diabetes in adolescence: a prospective cohort study. Assessment of gestational diabetes mellitus knowledge, attitudes, and practices and associated factors among pregnant women at a district hospital in Coastal Kenya.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1