T. Bose, T. G. Mahomed, K. C. Mbatha, J. C. Joubert, A. Hammerbacher
{"title":"组织发育和化学成分对黑杨木材和枝梢中细菌生物多样性的影响","authors":"T. Bose, T. G. Mahomed, K. C. Mbatha, J. C. Joubert, A. Hammerbacher","doi":"10.1111/plb.13724","DOIUrl":null,"url":null,"abstract":"<p>\n </p>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":"27 5","pages":"913-923"},"PeriodicalIF":3.6000,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/plb.13724","citationCount":"0","resultStr":"{\"title\":\"Tissue ontogeny and chemical composition influence bacterial biodiversity in the wood and shoot tip of Populus nigra\",\"authors\":\"T. Bose, T. G. Mahomed, K. C. Mbatha, J. C. Joubert, A. Hammerbacher\",\"doi\":\"10.1111/plb.13724\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>\\n </p>\",\"PeriodicalId\":220,\"journal\":{\"name\":\"Plant Biology\",\"volume\":\"27 5\",\"pages\":\"913-923\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-10-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/plb.13724\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/plb.13724\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/plb.13724","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
植物与微生物之间的相互作用对植物的生长动态和适应性有重大影响。本研究利用高通量测序和代谢物分析方法，探讨了在温室条件下代谢物对黑杨嫩枝尖和木材中微生物生物多样性的影响。黑杨的枝条被采收、生根并移植到花盆中生长。3 个月后，收集枝梢和木质部的组织样本，提取代谢物并使用 GC-MS 和 LC-MS 进行分析。提取基因组 DNA 并进行高通量测序，以分析细菌的生物多样性。利用生物信息学和统计学方法对这两个数据集进行了分析。代谢物分析表明，嫩枝尖端的初级和次级代谢物相对含量较高，包括糖类、脂肪酸、有机酸、酚酸衍生物和水杨酸类，而木质部则富含黄酮类化合物。这些组织的细菌生物多样性也有显著差异，芽尖主要是梭状芽孢杆菌属、类杆菌属和芽孢杆菌属，与快速生长和厌氧发酵有关，而木质部组织的特点是根瘤菌属、鞘氨醇单胞菌属和弗兰科菌属的重氮营养体。PCoA 聚类证实了特定组织的微生物差异。功能分析显示，芽尖富含基本的细胞过程，而木材则表现出与降解和死亡有关的途径。代谢物分析表明，初级和次级代谢物存在显著差异，凸显了它们对植物组织中微生物生物多样性的影响。每个组织中特定细菌群和不同功能途径的优势表明，微生物对嫩梢和木材的独特环境做出了量身定制的反应。
期刊介绍：
Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology.
Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
