Nanwei Wang, Wei Hu, Hui Jiang, Donglei Jiang, Lifeng Wang
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引用次数: 0
摘要
我们研究了一种三维打印肝脏微组织的便携式微纳通道生物传感器,用于快速灵敏地检测脱氧雪腐镰刀菌烯醇(DON)。用纳米多孔阳极氧化铝(AAO)、金纳米颗粒(AuNPs)和细胞色素 C 氧化酶(COx)修饰了丝网印刷碳电极(SPCE),以提高传感器的性能。明胶甲基丙烯酸酯水凝胶与肝癌细胞相结合,形成了三维打印的生物墨水。通过生物三维打印技术,利用标准化和高通量技术制备了肝脏微组织。这些微组织被固定在改良电极上,制成了肝脏微组织传感器。通过循环伏安法(CV)测定,该生物传感器的峰值电流与 DON 浓度呈正相关,线性检测范围为 2∼40 μg/mL。标准曲线方程为 ICV(μA) = = 18.76956 + 0.03107CDON(μg/mL),相关系数 R2 为 0.99471(n=3)。根据公式计算得出最低检测限为1.229 μg/mL,表明便携式微纳通道生物三维打印肝脏微组织生物传感器的构建获得成功。它为开发快速便捷的仪器检测粮食生产后的霉菌毒素危害提供了创新思路。它在预测和评估粮食产后质量变化方面也具有巨大的应用潜力。
A portable micro-nanochannel bio-3D printed liver microtissue biosensor for DON detection.
We investigated a portable micro-nanochannel biosensor 3D-printed liver microtissues for rapid and sensitive deoxynivalenol (DON) detection. The screen-printed carbon electrode (SPCE) was modified with nanoporous anodic aluminum oxide (AAO), gold nanoparticles (AuNPs), and cytochrome C oxidase (COx) to enhance sensor performance. Gelatin methacrylate hydrogel, combined with hepatocellular carcinoma cells, formed the bioink for 3D printing. Liver microtissues were prepared through standardized and high-throughput techniques via bio-3D printing technology. These microtissues were immobilized onto modified electrodes to fabricate liver microtissue sensors. The peak current of this biosensor was positively correlated with DON concentration, as determined by cyclic voltammetry (CV), within a linear detection range of 2∼40 μg/mL. The standard curve equation is denoted by ICV(μA) = = 18.76956 + 0.03107CDON(μg/mL), with a correlation coefficient R2 was 0.99471(n=3). A minimum detection limit of 1.229 μg/mL was calculated from the formula, indicating the successful construction of a portable micro-nanochannel bio-3D printed liver microtissue biosensor. It provides innovative ideas for developing rapid and convenient instrumentation to detect mycotoxin hazards after grain production. It also holds significant potential for application in the prediction and assessment of post-production quality changes in grain.
期刊介绍:
Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.