甲基苯丙胺诱发的记忆损伤和短暂的神经炎症:分析小鼠海马体在短期和长期暴露后的 mRNA 变化。

IF 4.6 2区 医学 Q1 NEUROSCIENCES Neuropharmacology Pub Date : 2024-09-30 DOI:10.1016/j.neuropharm.2024.110175
Laiqiang Wu , Xiaorui Liu , Qingchen Jiang , Ming Li , Min Liang , Shuai Wang , Rui Wang , Linlan Su , Tong Ni , Nan Dong , Li Zhu , Fanglin Guan , Jie Zhu , Wen Zhang , Min Wu , Yanjiong Chen , Teng Chen , Biao Wang
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引用次数: 0

摘要

甲基苯丙胺(METH)被认为会诱发记忆损伤,但这种效应的确切机制仍不清楚。目前的研究往往局限于单一的模型,或侧重于单个基因或蛋白质的功能,这阻碍了对潜在机制的全面了解。在本研究中,我们建立了三种 METH 暴露小鼠模型,提取了海马核,并利用 RNA 测序分析了 mRNA 表达谱的变化。我们的研究结果表明,METH 会严重损害小鼠的学习和记忆能力。此外,我们还观察到,METH 诱导的炎症反应发生在早期阶段,并不会随着重复注射而进一步加剧。然而,RNA 测序发现炎症通路分子持续富集,这与行为恶化有关。这表明,尽管 METH 诱导的神经炎症在学习和记忆损伤中起着关键作用,但炎症通路分子的持续富集与行为结果有关。这些发现为免疫干预在 METH 相关疾病中的潜在应用提供了重要证据。
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Methamphetamine-induced impairment of memory and fleeting neuroinflammation: Profiling mRNA changes in mouse hippocampus following short-term and long-term exposure
Methamphetamine (METH) has been implicated in inducing memory impairment, but the precise mechanisms underlying this effect remain unclear. Current research often limits itself to singular models or focuses on individual gene or protein functions, which hampers a comprehensive understanding of the underlying mechanisms. In this study, we established three METH mouse exposure models, extracted hippocampal nuclei, and utilized RNA sequencing to analyze changes in mRNA expression profiles. Our results indicate that METH significantly impairs the learning and memory capabilities of mice. Additionally, we observed that METH-induced inflammatory responses occur in the early phase and do not further exacerbate with repeated injections. However, RNA sequencing revealed the persistent enrichment of inflammatory pathway molecules, which correlated with worsened behaviors. This suggests that although METH-induced neuroinflammation plays a critical role in learning and memory impairment, the continued enrichment of inflammatory pathway molecules is associated with behavioral outcomes. These findings provide crucial evidence for the potential application of immune intervention in METH-related disorders.
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来源期刊
Neuropharmacology
Neuropharmacology 医学-神经科学
CiteScore
10.00
自引率
4.30%
发文量
288
审稿时长
45 days
期刊介绍: Neuropharmacology publishes high quality, original research and review articles within the discipline of neuroscience, especially articles with a neuropharmacological component. However, papers within any area of neuroscience will be considered. The journal does not usually accept clinical research, although preclinical neuropharmacological studies in humans may be considered. The journal only considers submissions in which the chemical structures and compositions of experimental agents are readily available in the literature or disclosed by the authors in the submitted manuscript. Only in exceptional circumstances will natural products be considered, and then only if the preparation is well defined by scientific means. Neuropharmacology publishes articles of any length (original research and reviews).
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