人类共生动物 Segatella copri 的 RNA 图谱揭示了肠道定植所必需的小 RNA

IF 20.6 1区 医学 Q1 MICROBIOLOGY Cell host & microbe Pub Date : 2024-10-04 DOI:10.1016/j.chom.2024.09.008
Youssef El Mouali, Caroline Tawk, Kun D. Huang, Lena Amend, Till Robin Lesker, Falk Ponath, Jörg Vogel, Till Strowig
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引用次数: 0

摘要

Segatella copri 细菌是人类肠道微生物群中的一种常见成员,与健康和疾病状态有关。然而,决定其有效定植肠道能力的内在因素在很大程度上仍然未知。通过对 S. copri 进行广泛的转录组图谱绘制和对人类来源样本的研究,我们发现了一种小 RNA,并将其命名为 Segatella RNA 定殖因子(SrcF)。SrcF 可调控涉及营养获取的基因,而复杂碳水化合物(尤其是果聚糖)可控制其表达。此外,SrcF 的表达还受到人类微生物群组成以及同居共生动物分解果聚糖的强烈影响,这表明复杂碳水化合物的分解在产生能量的既定功能之外,还介导了共生动物之间的种间信号传递。总之,这项研究强调了小 RNA 作为肠道定植关键调节因子的作用。
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The RNA landscape of the human commensal Segatella copri reveals a small RNA essential for gut colonization
The bacterium Segatella copri is a prevalent member of the human gut microbiota associated with health and disease states. However, the intrinsic factors that determine its ability to colonize the gut effectively remain largely unknown. By extensive transcriptome mapping of S. copri and examining human-derived samples, we discover a small RNA, which we name Segatella RNA colonization factor (SrcF), and show that SrcF is essential for S. copri gut colonization in gnotobiotic mice. SrcF regulates genes involved in nutrient acquisition, and complex carbohydrates, particularly fructans, control its expression. Furthermore, SrcF expression is strongly influenced by human microbiome composition and by the breakdown of fructans by cohabitating commensals, suggesting that the breakdown of complex carbohydrates mediates interspecies signaling among commensals beyond its established function in generating energy. Together, this study highlights the contribution of a small RNA as a critical regulator in gut colonization.
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来源期刊
Cell host & microbe
Cell host & microbe 生物-微生物学
CiteScore
45.10
自引率
1.70%
发文量
201
审稿时长
4-8 weeks
期刊介绍: Cell Host & Microbe is a scientific journal that was launched in March 2007. The journal aims to provide a platform for scientists to exchange ideas and concepts related to the study of microbes and their interaction with host organisms at a molecular, cellular, and immune level. It publishes novel findings on a wide range of microorganisms including bacteria, fungi, parasites, and viruses. The journal focuses on the interface between the microbe and its host, whether the host is a vertebrate, invertebrate, or plant, and whether the microbe is pathogenic, non-pathogenic, or commensal. The integrated study of microbes and their interactions with each other, their host, and the cellular environment they inhabit is a unifying theme of the journal. The published work in Cell Host & Microbe is expected to be of exceptional significance within its field and also of interest to researchers in other areas. In addition to primary research articles, the journal features expert analysis, commentary, and reviews on current topics of interest in the field.
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