杏仁核基底外侧副缬氨酸和胆囊收缩素表达的GABA能神经元调节抑郁和焦虑样行为

IF 5.8 1区 医学 Q1 PSYCHIATRY Translational Psychiatry Pub Date : 2024-10-05 DOI:10.1038/s41398-024-03135-z
Muhammad Asim, Huajie Wang, Abdul Waris, Jufang He
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引用次数: 0

摘要

人们越来越认识到,杏仁基底外侧(BLA)是抑郁和焦虑样行为的关键调节器。然而,人们对单个杏仁基底外侧神经元对这些行为的具体贡献仍然知之甚少。我们之前的研究表明,谷氨酸能BLA神经元对厌恶刺激的反应活动增加,而且增强BLA的抑制作用可以减轻抑郁样行为,在此基础上,我们研究了单个BLA GABA能神经元(BLAGABA)在抑郁和焦虑样表型中的作用。为了解决这个问题,我们采用了一系列综合技术,包括 c-fos 染色、纤维光度记录、光遗传和化学遗传操作以及行为分析。我们的研究结果表明,BLAGABA神经元在社会交往过程中的尾部悬吊和慢性社会挫败应激(CSDS)后活动减少。高频激活BLAGABA神经元可减轻抑郁和焦虑行为,而低频激活则没有影响。纤维光度记录显示,在脚震厌恶刺激时,BLA GABA能神经元表达躯体促肾上腺皮质素(SST)、副缬氨酸(PV)和胆囊收缩素(CCK)的活动增加。此外,我们还发现在尾部悬吊应激时,BLA中PV和SST神经元的活动增加,而CCK-GABA神经元的活动减少。然而,在 CSDS 后,BLAPV 神经元的活性降低了,而 SST 和 CCK 神经元在社会互动测试中的活性没有变化。行为分析表明,抑制PV和CCK-GABA神经元会诱发抑郁和焦虑行为,而抑制SST神经元则没有影响。相反,BLAPV神经元的化学激活可减轻抑郁行为,而BLACCK-GABA神经元的激活至少可部分减轻抑郁和焦虑行为。这项研究提供了令人信服的证据,证明 BLAPV 神经元在调节小鼠的抑郁样行为中起着关键作用,而 BLACCK-GABA 神经元至少部分参与调节小鼠的抑郁样行为和焦虑样行为。
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Basolateral amygdala parvalbumin and cholecystokinin-expressing GABAergic neurons modulate depressive and anxiety-like behaviors.

The basolateral amygdala (BLA) is increasingly recognized as a key regulator of depression and anxiety-like behaviors. However, the specific contribution of individual BLA neurons to these behaviors remains poorly understood. Building on our previous study, which demonstrated increased activity in glutamatergic BLA neurons in response to aversive stimuli and that enhancing inhibition in the BLA can alleviate depressive-like behaviors, we investigated the role of individual BLA GABAergic neurons (BLAGABA) in depressive and anxiety-like phenotypes. To address this question, we employed a comprehensive array of techniques, including c-fos staining, fiber photometry recording, optogenetic and chemogenetic manipulation, and behavior analysis. Our findings indicate that BLAGABA neurons show decreased activity during tail suspension and after chronic social defeat stress (CSDS) during social interaction. High-frequency activation of BLAGABA neurons attenuated depressive and anxiety-like behaviors, while low-frequency activation had no effect. Fiber photometry recordings revealed increased activity in BLA GABAergic neurons expressing somatostatin (SST), parvalbumin (PV), and cholecystokinin (CCK) during footshock aversive stimuli. Moreover, we found increased activity in PV and SST neurons and decreased activity in CCK-GABA neurons in the BLA during tail suspension stress. However, after CSDS, BLAPV neurons displayed decreased activity, while SST and CCK neurons showed no changes during the social interaction test. Behavioral analysis demonstrated that chemogenetic inhibition of PV and CCK-GABA neurons induced depressive and anxiety-like behaviors. whereas SST neuron inhibition had no effect. Conversely, chemogenetic activation of BLAPV neurons alleviated depressive behaviors, and activation of BLACCK-GABA neurons alleviated at least partly both depressive and anxiety-like behaviors. This study provides compelling evidence that BLAPV neurons play a critical role in regulating depressive-like behaviors, and that BLACCK-GABA neurons are involved, at least in part, in modulating both depressive-like and anxiety-like behaviors in mice.

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来源期刊
CiteScore
11.50
自引率
2.90%
发文量
484
审稿时长
23 weeks
期刊介绍: Psychiatry has suffered tremendously by the limited translational pipeline. Nobel laureate Julius Axelrod''s discovery in 1961 of monoamine reuptake by pre-synaptic neurons still forms the basis of contemporary antidepressant treatment. There is a grievous gap between the explosion of knowledge in neuroscience and conceptually novel treatments for our patients. Translational Psychiatry bridges this gap by fostering and highlighting the pathway from discovery to clinical applications, healthcare and global health. We view translation broadly as the full spectrum of work that marks the pathway from discovery to global health, inclusive. The steps of translation that are within the scope of Translational Psychiatry include (i) fundamental discovery, (ii) bench to bedside, (iii) bedside to clinical applications (clinical trials), (iv) translation to policy and health care guidelines, (v) assessment of health policy and usage, and (vi) global health. All areas of medical research, including — but not restricted to — molecular biology, genetics, pharmacology, imaging and epidemiology are welcome as they contribute to enhance the field of translational psychiatry.
期刊最新文献
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