耐甲氧西林金黄色葡萄球菌的分子多样性。

IF 3.7 Q2 INFECTIOUS DISEASES JAC-Antimicrobial Resistance Pub Date : 2024-10-05 eCollection Date: 2024-10-01 DOI:10.1093/jacamr/dlae154
Dalida Bivona, Emanuele Nicitra, Carmelo Bonomo, Maddalena Calvo, Giuseppe Migliorisi, Marianna Perez, Grete Francesca Privitera, Nicolò Musso, Stefania Stefani, Dafne Bongiorno
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引用次数: 0

摘要

目的:最近出现了耐药夫西地酸(FA)的金黄色葡萄球菌,这凸显了积极监测分离这些菌株的重要性。本研究通过表型和基因分型方法评估了四株边界耐草青霉素金黄色葡萄球菌(BORSA)临床菌株耐药的分子基础和带毒因子:所有金黄色葡萄球菌临床菌株均来自西西里岛的多家医院。进行了体外抗生素药敏试验。使用 Illumina MiSeq 平台进行了 WGS 分析,并对数据进行了分析,以确定 ST、抗性组和病毒组特征:结果:基因型特征分析显示,这些菌株属于四个ST:结果:基因型特征显示,菌株属于四个ST:ST630、ST8、ST15和ST1。对 FA 的抗性与 fusA 基因或 fusB 和 fusC 基因的突变有关。此外,一个病例表现出对莫匹罗星的耐药性,这与 mupA 基因的存在有关。在四个病例中,有三个病例的头孢西丁 MIC 值处于临界状态,因此被归类为 BORSA。毒力基因含量复杂多样,其中一个病例的lukS/F基因检测呈阳性,该基因编码PVL毒素:对 FA 的耐药性是多因素的,涉及染色体基因的点突变或与移动遗传因子的关联。监测对这些抗生素的耐药性可能有助于管理和根除对莫匹罗星和FA耐药的金黄色葡萄球菌菌株,众所周知,这些菌株也是毒力决定因素的重要载体。
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Molecular diversity in fusidic acid-resistant Methicillin Susceptible Staphylococcus aureus.

Objectives: The recent emergence of fusidic acid (FA)-resistant Staphylococcus aureus has underscored the importance of active surveillance in isolating these strains. The molecular basis of fusidic acid resistance and the carriage of virulence factors in four borderline oxacillin-resistant Staphylococcus aureus (BORSA) clinical strains was assessed through phenotypical and genotypical methods.

Methods: All S. aureus clinical strains were obtained from various hospital units in Sicily. In vitro antibiotic susceptibility testing was conducted. WGS was performed using the Illumina MiSeq Platform, and data analysis was carried out to determine ST, resistome and virulome profiles.

Results: Genotypic characterization revealed that the strains belong to four STs: ST630, ST8, ST15, and ST1. FA resistance was associated with mutations in the fusA gene or fusB and fusC genes. Additionally, one case exhibited resistance to mupirocin, related to the presence of the mupA gene. Borderline MIC values were observed for cefoxitin in three out of four cases, leading to their categorization as BORSA. Virulence gene content was complex and diversified, with one testing positive for the lukS/F genes, coding for PVL toxin.

Conclusions: Resistance to FA is multifactorial, involving point mutations in chromosomal genes or association with mobile genetic elements. Monitoring the resistance to these antibiotics might help to manage and eradicate mupirocin- and FA-resistant S. aureus strains, which are also known to be important carriers of virulence determinants.

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16 weeks
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