David Mazurais, Victor Simon, Pauline Auffret, Alexandre Cormier, Alexandra Dauvé, Lauriane Madec, Baptiste Tanguy-Guillo, Nicolas Gayet, Elodie Fleury, Jérémy Le Luyer
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Gene ontologies analyses of expressed genes support the evidence that the tongue exhibits biological processes related to the sensory system, tooth mineralization and immune defences among others. Our data revealed only 295 OA-induced regulated genes with 114 up- and 181 down-regulated by OA. Functions over-represented encompass processes involved in organic substance metabolic process, RNA metabolism and especially RNA methylation which, combined with the regulation of some hsp genes expression, suggest a molecular response to stress which might contribute to lingual cell homeostasis under OA. The immune system process is also found enriched within OA-induced regulated genes. With the exception of one fatty acid receptor, known taste perception effectors were not impacted by OA in the tongue. However, a complementary droplet digital PCR approach dedicated to genes involved in gustatory signal transduction revealed the down regulation by OA of pyrimidinergic receptor (p2ry4) transcript expression in the gills of the fish. Combined with scanning electron microscopy analysis, our RNAseq data revealed that OA has no impact on processes related to teeth development and mineralization. Altogether, our data reveal that multigenerational exposure to OA has not a substantially effect on the tongue transcriptome but emphasis should be placed on investigating the potential physiological consequences related to the regulation of genes related to cell stress, immune system and fatty acid sensitivity to conclude on species resilience in face of OA.</p>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mutligenerational chronic exposure to near future ocean acidification in European sea bass (Dicentrarchus labrax): Insights into the regulation of the transcriptome in a sensory organ involved in feed intake, the tongue.\",\"authors\":\"David Mazurais, Victor Simon, Pauline Auffret, Alexandre Cormier, Alexandra Dauvé, Lauriane Madec, Baptiste Tanguy-Guillo, Nicolas Gayet, Elodie Fleury, Jérémy Le Luyer\",\"doi\":\"10.1016/j.marenvres.2024.106775\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In this study, we examined the effect of near future ocean acidification (OA) on the transcriptome of a sensory organ in contact with surrounding water, the tongue in adult European sea bass (Dicentrarchus labrax) by mean of RNAseq experiment. 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引用次数: 0
摘要
在这项研究中,我们通过RNAseq实验研究了近期海洋酸化(OA)对欧洲鲈鱼(Dicentrarchus labrax)成鱼舌头这一与周围水接触的感觉器官转录组的影响。我们从实际海水 pH 值(pH 8.0 条件下)和预测的近未来海水 pH 值(pH 7.6 条件下)条件下饲养的两代鱼的舌头上获得了总计 14.1 Mb 的高质量修剪读数,涵盖 18,703 个表达基因。对表达基因的基因本体分析证明,鱼舌的生物过程与感觉系统、牙齿矿化和免疫防御等有关。我们的数据只发现了 295 个 OA 诱导的调控基因,其中 114 个上调,181 个下调。被高调的功能包括有机物质代谢过程、RNA 代谢过程,尤其是 RNA 甲基化过程,这些过程与一些 hsp 基因的表达调控结合在一起,表明在 OA 的作用下,分子对压力的反应可能有助于舌细胞的平衡。在 OA 诱导的调控基因中也发现了免疫系统过程。除一种脂肪酸受体外,已知的味觉感知效应因子并未受到舌头 OA 的影响。然而,一种专门针对味觉信号转导相关基因的互补液滴数字 PCR 方法显示,OA 下调了鱼鳃中嘧啶能受体(p2ry4)转录本的表达。结合扫描电子显微镜分析,我们的 RNAseq 数据显示,OA 对牙齿发育和矿化过程没有影响。总之,我们的数据显示,多代暴露于 OA 不会对舌头转录组产生实质性影响,但应重点研究与细胞应激、免疫系统和脂肪酸敏感性相关基因的调控有关的潜在生理后果,从而得出物种面对 OA 的恢复能力的结论。
Mutligenerational chronic exposure to near future ocean acidification in European sea bass (Dicentrarchus labrax): Insights into the regulation of the transcriptome in a sensory organ involved in feed intake, the tongue.
In this study, we examined the effect of near future ocean acidification (OA) on the transcriptome of a sensory organ in contact with surrounding water, the tongue in adult European sea bass (Dicentrarchus labrax) by mean of RNAseq experiment. We acquired a total of 14.1 Mb quality-trimmed reads covering 18,703 expressed genes from the tongue of fish reared from two generations at actual (pH 8.0 condition) and predicted near-future seawater pH (pH 7.6 condition). Gene ontologies analyses of expressed genes support the evidence that the tongue exhibits biological processes related to the sensory system, tooth mineralization and immune defences among others. Our data revealed only 295 OA-induced regulated genes with 114 up- and 181 down-regulated by OA. Functions over-represented encompass processes involved in organic substance metabolic process, RNA metabolism and especially RNA methylation which, combined with the regulation of some hsp genes expression, suggest a molecular response to stress which might contribute to lingual cell homeostasis under OA. The immune system process is also found enriched within OA-induced regulated genes. With the exception of one fatty acid receptor, known taste perception effectors were not impacted by OA in the tongue. However, a complementary droplet digital PCR approach dedicated to genes involved in gustatory signal transduction revealed the down regulation by OA of pyrimidinergic receptor (p2ry4) transcript expression in the gills of the fish. Combined with scanning electron microscopy analysis, our RNAseq data revealed that OA has no impact on processes related to teeth development and mineralization. Altogether, our data reveal that multigenerational exposure to OA has not a substantially effect on the tongue transcriptome but emphasis should be placed on investigating the potential physiological consequences related to the regulation of genes related to cell stress, immune system and fatty acid sensitivity to conclude on species resilience in face of OA.
期刊介绍:
Marine Environmental Research publishes original research papers on chemical, physical, and biological interactions in the oceans and coastal waters. The journal serves as a forum for new information on biology, chemistry, and toxicology and syntheses that advance understanding of marine environmental processes.
Submission of multidisciplinary studies is encouraged. Studies that utilize experimental approaches to clarify the roles of anthropogenic and natural causes of changes in marine ecosystems are especially welcome, as are those studies that represent new developments of a theoretical or conceptual aspect of marine science. All papers published in this journal are reviewed by qualified peers prior to acceptance and publication. Examples of topics considered to be appropriate for the journal include, but are not limited to, the following:
– The extent, persistence, and consequences of change and the recovery from such change in natural marine systems
– The biochemical, physiological, and ecological consequences of contaminants to marine organisms and ecosystems
– The biogeochemistry of naturally occurring and anthropogenic substances
– Models that describe and predict the above processes
– Monitoring studies, to the extent that their results provide new information on functional processes
– Methodological papers describing improved quantitative techniques for the marine sciences.