Ya-Zhou Wang, Ke-Rui Geng, Bing-Lan Mo, Ting Liu, Ye-Tao Tang, Dian Wen, Fu-Rong Li, Xu Wang, Lei Li, Rui-Ying Du, Antony van der Ent, Teng-Hao-Bo Deng, Jean-Louis Morel, Rong-Liang Qiu
{"title":"镍的高积累物 Odontarrhena chalcidica（十字花科）对锌的吸收优于对镍的吸收","authors":"Ya-Zhou Wang, Ke-Rui Geng, Bing-Lan Mo, Ting Liu, Ye-Tao Tang, Dian Wen, Fu-Rong Li, Xu Wang, Lei Li, Rui-Ying Du, Antony van der Ent, Teng-Hao-Bo Deng, Jean-Louis Morel, Rong-Liang Qiu","doi":"10.1007/s11104-024-06984-1","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background and aims</h3><p><i>Odontarrhena chalcidica</i> is a well-known nickel hyperaccumulator which also take up zinc efficiently, but little is known regarding its zinc uptake mechanisms. Therefore, this study compared the uptake kinetics of zinc and nickel.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>Plants were cultivated under various metals (nickel, zinc, cobalt and iron) concentrations of the hydroponic solutions. The plants underwent assessment for a range of parameters, including metal concentrations in the plant, expression level of selected metal transporters, and absorption kinetics models for nickel and zinc in the roots.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>The plants accumulated high concentrations of zinc in roots (5000 mg kg<sup>−1</sup>). Zinc uptake was not influenced by nickel, but nickel uptake was severely suppressed by zinc (drop by 87.4%), while significantly stimulated by zinc deficiency (increase by 30.4%). The root uptake of zinc and nickel fit Michaelis-Menten kinetics with <i>K</i><sub><i>m</i></sub> for zinc and nickel of 8.99 and 18.3 µM, respectively, while the <i>V</i><sub><i>max</i></sub> of nickel was approximately 1.8 times greater than that of zinc. The presence of zinc greatly decreased both the <i>V</i><sub><i>max</i></sub> and <i>K</i><sub><i>m</i></sub> of nickel. RT-Q-PCR results showed that zinc exposure only stimulated the gene expression levels of zinc transporter (e.g.,<i> ZIP10</i>), whilst nickel exposure stimulates gene expression levels of both zinc and iron transporters.</p><h3 data-test=\"abstract-sub-heading\">Conclusions</h3><p><i>O. chalcidica</i> preferentially takes up zinc over nickel, probably via relatively high-affinity transporters. Whilst nickel uptake is via a broader range of transporters, and the zinc transporters appear to be part of the nickel transport systems.</p>","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":"6 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The nickel hyperaccumulator Odontarrhena chalcidica (Brassicaceae) preferentially takes up zinc over nickel\",\"authors\":\"Ya-Zhou Wang, Ke-Rui Geng, Bing-Lan Mo, Ting Liu, Ye-Tao Tang, Dian Wen, Fu-Rong Li, Xu Wang, Lei Li, Rui-Ying Du, Antony van der Ent, Teng-Hao-Bo Deng, Jean-Louis Morel, Rong-Liang Qiu\",\"doi\":\"10.1007/s11104-024-06984-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Background and aims</h3><p><i>Odontarrhena chalcidica</i> is a well-known nickel hyperaccumulator which also take up zinc efficiently, but little is known regarding its zinc uptake mechanisms. Therefore, this study compared the uptake kinetics of zinc and nickel.</p><h3 data-test=\\\"abstract-sub-heading\\\">Methods</h3><p>Plants were cultivated under various metals (nickel, zinc, cobalt and iron) concentrations of the hydroponic solutions. The plants underwent assessment for a range of parameters, including metal concentrations in the plant, expression level of selected metal transporters, and absorption kinetics models for nickel and zinc in the roots.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>The plants accumulated high concentrations of zinc in roots (5000 mg kg<sup>−1</sup>). Zinc uptake was not influenced by nickel, but nickel uptake was severely suppressed by zinc (drop by 87.4%), while significantly stimulated by zinc deficiency (increase by 30.4%). The root uptake of zinc and nickel fit Michaelis-Menten kinetics with <i>K</i><sub><i>m</i></sub> for zinc and nickel of 8.99 and 18.3 µM, respectively, while the <i>V</i><sub><i>max</i></sub> of nickel was approximately 1.8 times greater than that of zinc. The presence of zinc greatly decreased both the <i>V</i><sub><i>max</i></sub> and <i>K</i><sub><i>m</i></sub> of nickel. RT-Q-PCR results showed that zinc exposure only stimulated the gene expression levels of zinc transporter (e.g.,<i> ZIP10</i>), whilst nickel exposure stimulates gene expression levels of both zinc and iron transporters.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusions</h3><p><i>O. chalcidica</i> preferentially takes up zinc over nickel, probably via relatively high-affinity transporters. Whilst nickel uptake is via a broader range of transporters, and the zinc transporters appear to be part of the nickel transport systems.</p>\",\"PeriodicalId\":20223,\"journal\":{\"name\":\"Plant and Soil\",\"volume\":\"6 1\",\"pages\":\"\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-10-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant and Soil\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11104-024-06984-1\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-024-06984-1","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
The nickel hyperaccumulator Odontarrhena chalcidica (Brassicaceae) preferentially takes up zinc over nickel
Background and aims
Odontarrhena chalcidica is a well-known nickel hyperaccumulator which also take up zinc efficiently, but little is known regarding its zinc uptake mechanisms. Therefore, this study compared the uptake kinetics of zinc and nickel.
Methods
Plants were cultivated under various metals (nickel, zinc, cobalt and iron) concentrations of the hydroponic solutions. The plants underwent assessment for a range of parameters, including metal concentrations in the plant, expression level of selected metal transporters, and absorption kinetics models for nickel and zinc in the roots.
Results
The plants accumulated high concentrations of zinc in roots (5000 mg kg−1). Zinc uptake was not influenced by nickel, but nickel uptake was severely suppressed by zinc (drop by 87.4%), while significantly stimulated by zinc deficiency (increase by 30.4%). The root uptake of zinc and nickel fit Michaelis-Menten kinetics with Km for zinc and nickel of 8.99 and 18.3 µM, respectively, while the Vmax of nickel was approximately 1.8 times greater than that of zinc. The presence of zinc greatly decreased both the Vmax and Km of nickel. RT-Q-PCR results showed that zinc exposure only stimulated the gene expression levels of zinc transporter (e.g., ZIP10), whilst nickel exposure stimulates gene expression levels of both zinc and iron transporters.
Conclusions
O. chalcidica preferentially takes up zinc over nickel, probably via relatively high-affinity transporters. Whilst nickel uptake is via a broader range of transporters, and the zinc transporters appear to be part of the nickel transport systems.
期刊介绍：
Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
