{"title":"含 8-羟基喹啉基团的罗丹明基荧光探针,用于高选择性检测 Hg2+,及其在细胞成像中的实际应用。","authors":"Lei Zhang, Jun Guo and Qihua You","doi":"10.1039/D4RA06115B","DOIUrl":null,"url":null,"abstract":"<p >A highly selective and sensitive fluorescent probe, <strong>RHOQ</strong>, was designed for the detection of Hg<small><sup>2+</sup></small> by incorporating an 8-hydroxyquinoline moiety onto a rhodamine molecular platform with a suitable linker. In MeOH–Tris (20 mM, pH = 7.4, 1 : 9, v/v) buffer solution, <strong>RHOQ</strong> exhibited 550-fold fluorescence enhancement at 594 nm upon addition of Hg<small><sup>2+</sup></small>, with a fast response and a low detection limit (9.67 × 10<small><sup>−8</sup></small> M). The 1 : 1 binding mode of <strong>RHOQ</strong> with Hg<small><sup>2+</sup></small> was established using Job's plot, UV-Vis, and fluorescence spectroscopic titration methods. Furthermore, <strong>RHOQ</strong> was successfully applied for the detection of Hg<small><sup>2+</sup></small> in living cells with good membrane permeability.</p>","PeriodicalId":102,"journal":{"name":"RSC Advances","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460212/pdf/","citationCount":"0","resultStr":"{\"title\":\"A rhodamine-based fluorescent probe bearing 8-hydroxyquinoline group for the highly selective detection of Hg2+ and its practical application in cell imaging†\",\"authors\":\"Lei Zhang, Jun Guo and Qihua You\",\"doi\":\"10.1039/D4RA06115B\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >A highly selective and sensitive fluorescent probe, <strong>RHOQ</strong>, was designed for the detection of Hg<small><sup>2+</sup></small> by incorporating an 8-hydroxyquinoline moiety onto a rhodamine molecular platform with a suitable linker. In MeOH–Tris (20 mM, pH = 7.4, 1 : 9, v/v) buffer solution, <strong>RHOQ</strong> exhibited 550-fold fluorescence enhancement at 594 nm upon addition of Hg<small><sup>2+</sup></small>, with a fast response and a low detection limit (9.67 × 10<small><sup>−8</sup></small> M). The 1 : 1 binding mode of <strong>RHOQ</strong> with Hg<small><sup>2+</sup></small> was established using Job's plot, UV-Vis, and fluorescence spectroscopic titration methods. Furthermore, <strong>RHOQ</strong> was successfully applied for the detection of Hg<small><sup>2+</sup></small> in living cells with good membrane permeability.</p>\",\"PeriodicalId\":102,\"journal\":{\"name\":\"RSC Advances\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-10-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460212/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"RSC Advances\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/ra/d4ra06115b\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"RSC Advances","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/ra/d4ra06115b","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
A rhodamine-based fluorescent probe bearing 8-hydroxyquinoline group for the highly selective detection of Hg2+ and its practical application in cell imaging†
A highly selective and sensitive fluorescent probe, RHOQ, was designed for the detection of Hg2+ by incorporating an 8-hydroxyquinoline moiety onto a rhodamine molecular platform with a suitable linker. In MeOH–Tris (20 mM, pH = 7.4, 1 : 9, v/v) buffer solution, RHOQ exhibited 550-fold fluorescence enhancement at 594 nm upon addition of Hg2+, with a fast response and a low detection limit (9.67 × 10−8 M). The 1 : 1 binding mode of RHOQ with Hg2+ was established using Job's plot, UV-Vis, and fluorescence spectroscopic titration methods. Furthermore, RHOQ was successfully applied for the detection of Hg2+ in living cells with good membrane permeability.
期刊介绍:
An international, peer-reviewed journal covering all of the chemical sciences, including multidisciplinary and emerging areas. RSC Advances is a gold open access journal allowing researchers free access to research articles, and offering an affordable open access publishing option for authors around the world.