不同出生体重新生儿脐带沃顿果冻中的 miRNA:一项试验性研究。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-10-05 DOI:10.1016/j.gene.2024.148984
Cristina García-Muro , Cristina Toledo-Gotor , Sara Pasamón-García , María de Pablo-de las Heras , Eduardo Esteban-Zubero , María Yolanda Ruiz del Prado , Elena Domínguez-Garrido
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引用次数: 0

摘要

背景:出生体重是围产期健康的重要指标:出生体重是围产期健康的一个重要指标。miRNA 是长度为 18 至 25 个核苷酸的非编码 RNA 小分子,可调控基因表达。特定的 miRNA 与影响胎儿生长的代谢途径有关,它们的失调可能会导致出生体重的变化。我们的目的是从脐带组织中分离出可扩增的 miRNA,并比较它们在三组患者中的表达情况:研究样本包括 23 名患者:方法:研究样本包括 23 名患者:8 名小胎龄(SGA)、10 名大胎龄(LGA)和 5 名适龄(AGA)。脐带组织样本在分娩后立即采集、储存,随后进行处理。利用高通量测序技术分析了这些样本的 miRNA 表达谱,并通过生物信息学分析对结果进行了评估:与 AGA 和 LGA 组相比,SGA 组的 miR-324-3p 表达下调。相反,与 SGA 和 AGA 相比,miR-337-3p 在 LGA 中上调。与 SGA 和 AGA 相比,miR-760 在 LGA 中下调,而与 AGA 相比,miR-4707-3p、miR-548a-3p 和 miR-6733-5p 在 SGA 和 LGA 中上调。这些miRNA中的大多数似乎与转化生长因子-β信号通路有关:这项探索性研究表明,脐带组织中 miRNA 的表达与出生体重有关。值得注意的是,miR-324-3p 在 SGA 新生儿中的下调表明,它的表达减少可能与 SGA 状况有关。
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miRNAs in umbilical Wharton’s jelly in neonates with different birth weights: A pilot study

Background

Birth weight is a critical indicator of perinatal health. miRNAs are small non-coding RNA molecules, ranging from 18 to 25 nucleotides in length, that regulate gene expression. Specific miRNAs have been implicated in metabolic pathways influencing fetal growth, and their dysregulation may contribute to variations in birth weight. Our objective was to isolate amplifiable miRNAs from umbilical cord tissue and compare their expression across three patient groups.

Methods

The study sample comprised 23 patients: 8 small for gestational age (SGA), 10 large for gestational age (LGA), and 5 appropriate for gestational age (AGA). Umbilical cord tissue samples were collected immediately after childbirth, stored, and subsequently processed. The miRNA expression profile of these samples was analyzed using high-throughput sequencing, and the results were evaluated through bioinformatic analysis.

Results

We identified significant differences in the expression levels of 6 miRNAs. miR-324-3p was downregulated in SGA compared to both AGA and LGA groups. Conversely, miR-337-3p was upregulated in LGA compared to both SGA and AGA. miR-760 was downregulated in LGA relative to SGA and AGA, while miR-4707-3p, miR-548a-3p, and miR-6733-5p were upregulated in both SGA and LGA compared to AGA. Most of these miRNAs appear to be associated with the transforming growth factor-beta signaling pathway.

Conclusions

This exploratory study suggests that miRNA expression in umbilical cord tissue is associated with birth weight. Notably, the downregulation of miR-324-3p in SGA newborns indicates that its decreased expression may be related to SGA conditions.
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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