在脊髓束损伤大鼠模型中减少神经性疼痛的睾酮信号通路

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI:10.22038/ijbms.2024.78491.16968
Abbas Alimoradian, Fatemeh Abbaszadeh, Masoumeh Jorjani
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引用次数: 0

摘要

目标:大多数脊髓损伤(SCI)患者都会出现神经性疼痛,目前尚无有效的治疗方法。在这项研究中,我们探讨了睾酮如何影响 SCI 引起的神经性疼痛:我们给脊髓束电解损伤后的雄性大鼠注射了三种不同剂量的睾酮(4、8、16 毫克/千克,腹腔注射)。然后,我们在脊髓损伤后28天内进行了行为测试,包括开阔地和von Frey测试。在脊髓损伤后第28天,我们用Western印迹法分析脊髓组织,测量损伤部位的电离钙结合适配分子1(Iba1)、胶质纤维酸性蛋白(GFAP)、磷酸胞外信号调节激酶(p-ERK1/2)和p-P38的水平:结果表明,与单纯脊髓损伤组相比,睾酮能明显改善运动活动和机械异感。睾酮还能抑制小胶质细胞和星形胶质细胞的活化。此外,睾酮还能明显降低 p-P38 和 p-ERK 水平:研究结果表明,睾酮可通过抑制星形胶质细胞和小胶质细胞的活化以及抑制 MAPK 信号通路来减轻 SCI 引起的神经病理性疼痛。
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Testosterone signaling pathways for reducing neuropathic pain in a rat model of spinothalamic tract lesion.

Objectives: Most individuals who suffer from spinal cord injury (SCI) experience neuropathic pain, which currently has no effective treatment. In this study, we examined how testosterone affects neuropathic pain resulting from SCI.

Materials and methods: We administered three different doses of testosterone (4, 8, 16 mg/kg, intraperitoneal) to male rats after an electrolytic lesion of the spinothalamic tract. We then conducted behavioral tests, including open field and von Frey tests, within 28 days post-SCI. On day 28 after SCI, we analyzed spinal tissue using western blot to measure the levels of ionized calcium binding adaptor molecule 1 (Iba1), glial fibrillary acidic protein (GFAP), phospho-extracellular signal-regulated kinase (p-ERK1/2), and p-P38 at the injury site.

Results: The results showed that testosterone significantly improved both motor activity and mechanical allodynia compared to the SCI-only group. Testosterone also inhibited microglia and astrocyte activation. Furthermore, testosterone significantly decreased p-P38 and p-ERK levels.

Conclusion: The findings indicate that testosterone may alleviate SCI-induced neuropathic pain by inhibiting the activation of astrocytes and microglia, as well as suppressing MAPK signaling pathways.

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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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