装载大麻二酚的可注射水凝胶可在体外调节巨噬细胞极化,用于治疗慢性肠炎。

IF 3.1 4区 医学 Q2 BIOPHYSICS Journal of Applied Biomaterials & Functional Materials Pub Date : 2024-01-01 DOI:10.1177/22808000241289022
Ye Wang, Xingming Ji, Xinyi Wang, Mengyu Sun, Cheng Li, Dongmei Wu
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引用次数: 0

摘要

目的:慢性肠病具有复发率高、病程长、久治不愈等特点,近年来发病率逐年上升。大麻二酚(CBD)具有显著的抗炎和抗氧化活性,但受限于其脂溶性和低生物利用度的特点。本研究旨在通过制备一种负载CBD的水凝胶系统(GelMA + CBD)来治疗慢性炎症性肠病:本研究设计并构建了GelMA + CBD,通过扫描电镜观察了其表面形态,并对其孔径、膨胀率和释放率进行了评估,以评价其生物活性和生物安全性。用酶联免疫吸附法检测各种炎症因子的表达,用激光共聚焦显微镜观察蛋白质和活性氧的表达,以评价其抗炎和抗氧化特性:研究发现,GelMA+CBD具有生物安全性,能使CBD缓慢释放,有效抑制体外巨噬细胞的M1型极化,促进M2型极化。此外,GelMA + CBD 还能减少巨噬细胞中促炎因子(如 iNOS)的表达,增加抗炎因子(如 Arg-1)的表达,清除细胞内活性氧(ROS),缓解氧化应激:体外实验证实,CBD负载水凝胶系统具有良好的生物安全性,可通过调节巨噬细胞的极化方向,进而抑制促炎因子的分泌,缓解炎症反应,为慢性肠炎的治疗奠定了坚实的基础。
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The injectable hydrogel loading cannabidiol to regulate macrophage polarization in vitro for the treatment of chronic enteritis.

Objective: Chronic bowel disease has the characteristics of high recurrence rate, prolonged and non-healing, and the incidence has increased year by year in recent years. Cannabidiol (CBD) has significant anti-inflammatory and antioxidant activities, but it is limited by its characteristics of fat solubility and low bioavailability. This study aims to treat chronic inflammatory bowel disease by preparing a CBD-loaded hydrogel system (GelMA + CBD) that can deliver CBD in situ and improve its bioavailability through slow release.

Method: The study designed and constructed GelMA + CBD, and its surface morphology was observed by scanning electron microscopy, and its pore size, swelling rate and release rate were evaluated to evaluate its bioactivity and biosafety. The expression of various inflammatory factors was detected by ELISA, and the expression of protein and reactive oxygen species were observed by laser confocal microscopy to evaluate their anti-inflammatory and antioxidant properties.

Results: Our study found that GelMA + CBD with biosafety, could make CBD be slowly released, and effectively inhibit the M1-type polarization of macrophages in vitro, and promote the M2-type polarization. In addition, GelMA + CBD can also reduce the expression of pro-inflammatory factors (such as iNOS) in macrophages, and increase the expression of anti-inflammatory factors (such as Arg-1), clear intracellular reactive oxygen species (ROS), and relieve oxidative stress.

Conclusion: The vitro experiments have confirmed that the CBD-loaded hydrogel system has good biosafety, and can alleviate inflammation by regulating the polarization direction of macrophages, and then inhibiting the secretion of pro-inflammatory factors, laying a strong foundation for the treatment of chronic enteritis.

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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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