光生物调制调节人成骨细胞的增殖和与钙信号相关的基因表达

IF 1.4 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING Journal of lasers in medical sciences Pub Date : 2024-09-14 eCollection Date: 2024-01-01 DOI:10.34172/jlms.2024.45
Fernando Russo Costa do Bomfim, Valéria Regina Gonzalez Sella, Ronaldo Luis Thomasini, Hélio Plapler
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引用次数: 0

摘要

简介用低强度激光进行光生物调节可刺激成骨细胞的细胞分裂,增加蛋白质的沉积量,从而促进新骨的形成。本研究旨在评估低强度激光光生物调制对人类成骨细胞增殖和钙信号相关基因表达的影响。研究方法培育人成骨细胞 hFOB1.19 系,并将其分为两组:对照组(C;培养孔数=78)和光生物调制组(L;培养孔数=78),实验期间每天培养孔数=6。培养细胞(在 34 ºC 温度下未成熟),在 37 ºC 温度下成熟后,L 组细胞接受低强度激光装置(砷化镓和铝)的激光照射,波长为 808 nm,输出功率为 200 mW,功率密度为 200 mW/cm2。照射到细胞上的能量为 37 焦耳/平方厘米,光束面积为 0.02 平方毫米,照射时间为 5 秒钟。这种治疗每天进行一次,持续 13 天。之后,对细胞数量进行计数,并分离、测量 RNA,然后将其转化为 cDNA,利用实时聚合酶链反应的比较 Ct 法进一步量化。然后通过 Mann-Whitney 检验对结果进行统计分析,显著性水平为 PResults:与对照组(22.75x10±4±7.660)相比,L 组的细胞数(37.25x10±4±22.02)在统计学上更高,P 值为 0.0259。S100A6、质膜钙 ATP 酶(PMCA)和钙调素基因的 2-ΔΔCt 值在第 13 天显示高表达,而骨钙素基因显示低表达。结论该研究表明,低强度激光的光生物调控机制可通过剂量依赖和累积的方式调节人成骨细胞的基因表达。
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Photobiomodulation Modulates Proliferation and Gene Expression Related to Calcium Signaling in Human Osteoblast Cells.

Introduction: Photobiomodulation with low-level laser treatment can enhance bone formation by stimulating the cell division of osteoblasts and increasing the amount of protein deposition, thus encouraging the formation of new bone. The aim of this study was to evaluate the effects of photobiomodulation with a low-level laser on proliferation and gene expression related to calcium signaling in human osteoblasts. Methods: Osteoblastic cell lines of the hFOB1.19 lineage, human osteoblasts, were grown and assigned into two groups, control (C; n=78 cultured wells) and photobiomodulation (L; n=78 cultured wells) with n=6 per day of the experimental period. Cells were cultured (immature at 34 ºC), and after maturation at 37 ºC, group L cells were exposed to laser irradiation with a low-level laser device (gallium and aluminum arsenide), at a wavelength of 808 nm, a power output of 200 mW, and a power density of 200 mW/cm2. The energy delivered to the cells was 37 J/cm2, with a beam area of 0.02 mm2 and an exposure time of 5 seconds. This treatment was applied daily for a period of 13 days. Following this, the number of cells was counted, and RNA was isolated, measured, and then converted into cDNA for further quantification using a comparative Ct method with real-time polymerase chain reaction. The results were then subjected to statistical analysis through a Mann-Whitney test, with a significance level of P<0.05. Results: The cell count in the L group (37.25x10±4±22.02) was statistically higher compared to the control group (22.75x10±4±7.660) with a P value of 0.0259. The values of 2-ΔΔCt for S100A6, plasma membrane calcium ATPase (PMCA), and calmodulin genes indicated hyper-expression on the thirteenth day, while the osteocalcin gene showed hypo-expression. Conclusion: The study suggests that the photobiomodulation mechanism with a low-level laser may regulate gene expression in human osteoblasts in a dose-dependent and cumulative manner.

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来源期刊
Journal of lasers in medical sciences
Journal of lasers in medical sciences RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
3.00
自引率
13.30%
发文量
24
期刊介绍: The "Journal of Lasers in Medical Sciences " is a scientific quarterly publication of the Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences. This journal received a scientific and research rank from the national medical publication committee. This Journal accepts original papers, review articles, case reports, brief reports, case series, photo assays, letters to the editor, and commentaries in the field of laser, or light in any fields of medicine such as the following medical specialties: -Dermatology -General and Vascular Surgery -Oncology -Cardiology -Dentistry -Urology -Rehabilitation -Ophthalmology -Otorhinolaryngology -Gynecology & Obstetrics -Internal Medicine -Orthopedics -Neurosurgery -Radiology -Pain Medicine (Algology) -Basic Sciences (Stem cell, Cellular and Molecular application and physic)
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