Runzi Zhang, Shunbi Xie, Qianyan Zhang, Ying Gao, Xiang He, Shanshan Jin, Junhao Leng, lian Zhang, Yi He
{"title":"由 EDTA 驱动的 \"爆炸性 \"CaCO3 微胶囊与 \"信号增强器 \"半导体 Zn0.995Ce0.005O SERS 基底相结合,用于氯霉素的超灵敏 SERS 检测","authors":"Runzi Zhang, Shunbi Xie, Qianyan Zhang, Ying Gao, Xiang He, Shanshan Jin, Junhao Leng, lian Zhang, Yi He","doi":"10.1016/j.aca.2024.343308","DOIUrl":null,"url":null,"abstract":"<h3>Background</h3>Chloramphenicol (CAP) is a broad-spectrum antibiotic, and its continuous use in human medicine, livestock has resulted disturbances in ecosystem stability. The complex background and low concentration of CAP in aquatic environments present significant scientific challenges for its sensitive detection. Currently detection techniques such as high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) are hindered by their complex procedures and high costs. Surface-enhanced Raman spectroscopy (SERS), due to its unique ability for fingerprint recognition, has emerged as a powerful tool for analysis and detection. However, it is often limited by the low kurtosis expression of the target.<h3>Result</h3>The water-soluble explosive polyethylenimide grafted calcium carbonate (PEI@CaCO3) microcapsule was utilized to encapsulate the signal probe Ag@4-NTP and combine it with a semiconductor SERS substrate Zn<sub>0.995</sub>Ce<sub>0.005</sub>O, enabling ultra-sensitive detection of CAP through a signal attenuation strategy. Moreover, magnetic molecularly imprinted polymers (MMIP) and nucleic acid aptamers were employed as capture probes for achieving rapid and direct magnetic separation, followed by layer-by-layer assembly to construct the sensor probe. In the presence of CAP, the aptamers and rMMIPs selectively recognized the target, forming a PEI@CaCO<sub>3</sub>@Ag@4-NTP@Apt@CAP@rMMIPs \"sandwich\" structure (Microcapsule@Apt@rMMIPs). After multiple magnetic separations, the \"igniter\" EDTA solution was added, causing the CaCO<sub>3</sub> capsules to rapidly \"explode\" and release a significant amount of Ag@4-NTP. This was then applied to the \"signal booster\" semiconductor SERS substrate. Under optimal conditions, this method exhibited a detection range of 1.0 × 10<sup>-5</sup> M to 1.0 × 10<sup>-15</sup> M, with a detection limit of 6.84×10<sup>-16</sup> M.<h3>Significant</h3>The unite of magnetic MIP and nucleic acid aptamer to form a “sandwich” structure, in combination with a semiconductor SERS substrate, not only enhances the sensor's sensitivity but also offers significant economic advantages. Moreover, this innovative technology holds great promise for accurate and highly sensitive detection of pollutants in complex environments.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"\\\"Explosive\\\" CaCO3 microcapsules driven by EDTA combined with a \\\"signal booster\\\" semiconductor Zn0.995Ce0.005O SERS substrate for ultra-sensitive SERS detection of chloramphenicol\",\"authors\":\"Runzi Zhang, Shunbi Xie, Qianyan Zhang, Ying Gao, Xiang He, Shanshan Jin, Junhao Leng, lian Zhang, Yi He\",\"doi\":\"10.1016/j.aca.2024.343308\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3>Background</h3>Chloramphenicol (CAP) is a broad-spectrum antibiotic, and its continuous use in human medicine, livestock has resulted disturbances in ecosystem stability. The complex background and low concentration of CAP in aquatic environments present significant scientific challenges for its sensitive detection. Currently detection techniques such as high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) are hindered by their complex procedures and high costs. Surface-enhanced Raman spectroscopy (SERS), due to its unique ability for fingerprint recognition, has emerged as a powerful tool for analysis and detection. However, it is often limited by the low kurtosis expression of the target.<h3>Result</h3>The water-soluble explosive polyethylenimide grafted calcium carbonate (PEI@CaCO3) microcapsule was utilized to encapsulate the signal probe Ag@4-NTP and combine it with a semiconductor SERS substrate Zn<sub>0.995</sub>Ce<sub>0.005</sub>O, enabling ultra-sensitive detection of CAP through a signal attenuation strategy. Moreover, magnetic molecularly imprinted polymers (MMIP) and nucleic acid aptamers were employed as capture probes for achieving rapid and direct magnetic separation, followed by layer-by-layer assembly to construct the sensor probe. In the presence of CAP, the aptamers and rMMIPs selectively recognized the target, forming a PEI@CaCO<sub>3</sub>@Ag@4-NTP@Apt@CAP@rMMIPs \\\"sandwich\\\" structure (Microcapsule@Apt@rMMIPs). After multiple magnetic separations, the \\\"igniter\\\" EDTA solution was added, causing the CaCO<sub>3</sub> capsules to rapidly \\\"explode\\\" and release a significant amount of Ag@4-NTP. This was then applied to the \\\"signal booster\\\" semiconductor SERS substrate. Under optimal conditions, this method exhibited a detection range of 1.0 × 10<sup>-5</sup> M to 1.0 × 10<sup>-15</sup> M, with a detection limit of 6.84×10<sup>-16</sup> M.<h3>Significant</h3>The unite of magnetic MIP and nucleic acid aptamer to form a “sandwich” structure, in combination with a semiconductor SERS substrate, not only enhances the sensor's sensitivity but also offers significant economic advantages. 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"Explosive" CaCO3 microcapsules driven by EDTA combined with a "signal booster" semiconductor Zn0.995Ce0.005O SERS substrate for ultra-sensitive SERS detection of chloramphenicol
Background
Chloramphenicol (CAP) is a broad-spectrum antibiotic, and its continuous use in human medicine, livestock has resulted disturbances in ecosystem stability. The complex background and low concentration of CAP in aquatic environments present significant scientific challenges for its sensitive detection. Currently detection techniques such as high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) are hindered by their complex procedures and high costs. Surface-enhanced Raman spectroscopy (SERS), due to its unique ability for fingerprint recognition, has emerged as a powerful tool for analysis and detection. However, it is often limited by the low kurtosis expression of the target.
Result
The water-soluble explosive polyethylenimide grafted calcium carbonate (PEI@CaCO3) microcapsule was utilized to encapsulate the signal probe Ag@4-NTP and combine it with a semiconductor SERS substrate Zn0.995Ce0.005O, enabling ultra-sensitive detection of CAP through a signal attenuation strategy. Moreover, magnetic molecularly imprinted polymers (MMIP) and nucleic acid aptamers were employed as capture probes for achieving rapid and direct magnetic separation, followed by layer-by-layer assembly to construct the sensor probe. In the presence of CAP, the aptamers and rMMIPs selectively recognized the target, forming a PEI@CaCO3@Ag@4-NTP@Apt@CAP@rMMIPs "sandwich" structure (Microcapsule@Apt@rMMIPs). After multiple magnetic separations, the "igniter" EDTA solution was added, causing the CaCO3 capsules to rapidly "explode" and release a significant amount of Ag@4-NTP. This was then applied to the "signal booster" semiconductor SERS substrate. Under optimal conditions, this method exhibited a detection range of 1.0 × 10-5 M to 1.0 × 10-15 M, with a detection limit of 6.84×10-16 M.
Significant
The unite of magnetic MIP and nucleic acid aptamer to form a “sandwich” structure, in combination with a semiconductor SERS substrate, not only enhances the sensor's sensitivity but also offers significant economic advantages. Moreover, this innovative technology holds great promise for accurate and highly sensitive detection of pollutants in complex environments.
期刊介绍:
Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.