适应变化:解决 NCK1 和 NCK2 的动态和双重作用。

IF 4.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical Journal Pub Date : 2024-10-16 DOI:10.1042/BCJ20230232
Valentine Teyssier, Casey R Williamson, Erka Shata, Stephanie P Rosen, Nina Jones, Nicolas Bisson
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引用次数: 0

摘要

适配蛋白在分子复合物的组装和特定通路的协调激活中发挥着核心作用。通过模块化结构域,NCK 家族的适体蛋白(NCK1 和 NCK2)通过单个 SRC 同源(SH)2 结构域和三个 SH3 结构域连接蛋白质靶标。通常,它们的 SH2 结构域与含磷酸酪氨酸基序的受体(如受体酪氨酸激酶)结合,而它们的 SH3 结构域则与含多脯氨酸基序的细胞质效应物结合。由于 NCK1 和 NCK2 都具有这些功能,因此人们错误地认为它们的作用是多余的。然而,与以前的观点相反,NCK1 和 NCK2 现在具有越来越多的旁系特异性功能,这突出表明有必要进一步探讨它们的差异。在这里,我们回顾了目前的证据,详细说明了这两个旁系亲属的独特性,包括它们在基因/蛋白调控、结合伙伴和对细胞功能的总体贡献方面的差异。为了帮助解释这些截然不同的特征,我们接着讨论了 SH2/SH3 的结构特征、无序的域间连接区和翻译后修饰。本综述旨在强调区分 NCK1 和 NCK2 在研究中的重要性,并为研究它们相互作用特异性的起源铺平道路。
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Adapting to change: resolving the dynamic and dual roles of NCK1 and NCK2.

Adaptor proteins play central roles in the assembly of molecular complexes and co-ordinated activation of specific pathways. Through their modular domain structure, the NCK family of adaptor proteins (NCK1 and NCK2) link protein targets via their single SRC Homology (SH) 2 and three SH3 domains. Classically, their SH2 domain binds to phosphotyrosine motif-containing receptors (e.g. receptor tyrosine kinases), while their SH3 domains bind polyproline motif-containing cytoplasmic effectors. Due to these functions being established for both NCK1 and NCK2, their roles were inaccurately assumed to be redundant. However, in contrast with this previously held view, NCK1 and NCK2 now have a growing list of paralog-specific functions, which underscores the need to further explore their differences. Here we review current evidence detailing how these two paralogs are unique, including differences in their gene/protein regulation, binding partners and overall contributions to cellular functions. To help explain these contrasting characteristics, we then discuss SH2/SH3 structural features, disordered interdomain linker regions and post-translational modifications. Together, this review seeks to highlight the importance of distinguishing NCK1 and NCK2 in research and to pave the way for investigations into the origins of their interaction specificity.

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来源期刊
Biochemical Journal
Biochemical Journal 生物-生化与分子生物学
CiteScore
8.00
自引率
0.00%
发文量
255
审稿时长
1 months
期刊介绍: Exploring the molecular mechanisms that underpin key biological processes, the Biochemical Journal is a leading bioscience journal publishing high-impact scientific research papers and reviews on the latest advances and new mechanistic concepts in the fields of biochemistry, cellular biosciences and molecular biology. The Journal and its Editorial Board are committed to publishing work that provides a significant advance to current understanding or mechanistic insights; studies that go beyond observational work using in vitro and/or in vivo approaches are welcomed. Painless publishing: All papers undergo a rigorous peer review process; however, the Editorial Board is committed to ensuring that, if revisions are recommended, extra experiments not necessary to the paper will not be asked for. Areas covered in the journal include: Cell biology Chemical biology Energy processes Gene expression and regulation Mechanisms of disease Metabolism Molecular structure and function Plant biology Signalling
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