Jingfei Fu, Rui Zhao, Yiyang Jiang, Yingyi Chen, Juan Du, Yi Liu, Junji Xu
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引用次数: 0
摘要
背景：过敏性接触性皮炎（ACD）是一种皮肤炎症性疾病，由接触皮肤的物质引起的过敏反应所致。T 细胞的过度激活在其发病机制中起着重要作用。光生物调节（PBM）是抑制炎症性疾病的一种有效治疗方法：本研究旨在评估 PBM 在 ACD 模型中的潜在有益作用，并探讨其可能的机制：本研究制作了 C57BL/6 小鼠 ACD 模型，并用 PBM 治疗，评估了 T 细胞的数量。在体外研究中，分离出幼稚 T 细胞并用 PBM 进行干预。用流式细胞仪检测 T 细胞活化的标志物。检测转化生长因子-β（TGF-β）和活性氧（ROS）以研究其机制：结果：PBM能有效抑制ACD模型中T细胞的数量，从而抑制炎症反应。体外研究表明，PBM 可直接缓和幼稚 T 细胞的活化，并具有通过 TGF-beta 信号通路阻碍 T 细胞活化的能力：我们的发现阐明了 PBM 在炎症性疾病中发挥抑制作用的潜在机制，为其临床应用奠定了理论基础。
Photobiomodulation suppresses allergic contact dermatitis by inhibiting T-cell activation.
Background: Allergic contact dermatitis (ACD) is a dermal inflammatory disease caused by allergic reactions to substances that contact the skin. The hyperactivation of T cells plays an important role in its pathogenesis. Photobiomodulation (PBM) is an efficacious therapeutic approach for suppressing inflammatory diseases.
Objective: This study aimed to evaluate the potentially beneficial role of PBM in ACD models and investigate its possible mechanisms.
Methods: In this study, the ACD model of C57BL/6 mice was produced and treated with PBM, and the number of T cells was evaluated. In an in vitro study, naïve T cells were isolated and intervened with PBM. The markers of T cell activation were detected by flow cytometer. Transforming growth factor-β (TGF-β) and reactive oxygen species (ROS) were detected to investigate the mechanism.
Results: PBM effectively inhibited the inflammatory response by impeding the number of T cells in the ACD model. And in vitro studies showed that PBM could directly moderate the activation of naïve T cells and possess the capability to impede T cell activation via TGF-beta signaling pathway.
Conclusion: Our finding elucidates the potential mechanism underlying the inhibitory effects of PBM in inflammatory diseases and furnishes a theoretical foundation for its clinical application.
期刊介绍：
Contact Dermatitis is designed primarily as a journal for clinicians who are interested in various aspects of environmental dermatitis. This includes both allergic and irritant (toxic) types of contact dermatitis, occupational (industrial) dermatitis and consumers" dermatitis from such products as cosmetics and toiletries. The journal aims at promoting and maintaining communication among dermatologists, industrial physicians, allergists and clinical immunologists, as well as chemists and research workers involved in industry and the production of consumer goods. Papers are invited on clinical observations, diagnosis and methods of investigation of patients, therapeutic measures, organisation and legislation relating to the control of occupational and consumers".
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