{"title":"马尼拉蛤血细胞对两种在宿主体内具有截然不同增殖动态的珀金斯鱼(P. Olseni 和 p. Mediterraneus)的反应中吞噬体酸化的差异。","authors":"Vince Neil B. Fuertes, Yuho Watanabe, Naoki Itoh","doi":"10.1016/j.jip.2024.108216","DOIUrl":null,"url":null,"abstract":"<div><div>Understanding the factors determining the host ranges of <em>Perkinsus</em> spp., a significant group of pathogenic protozoans affecting shellfish, is essential for preventing their spread and designing effective control measures. Considering that differences in the ability to proliferate within the host may influence the determination of host range, we first injected six <em>Perkinsus</em> spp. into Manila clams <em>Ruditapes philippinarum</em> and monitored the variations of trophozoite numbers. Although all six species were detected in the challenged clams 28 days post infection, the infection intensities varied among species, and particularly two species showed contrasting infection trends: <em>P. mediterraneus</em> showed a decreasing trend of infection, declining to the lowest intensity, whereas that of <em>P. olseni</em> continuously increased, reaching the highest intensity. <em>In vitro</em> exposure to Manila clam hemocytes revealed that the survival of <em>P. mediterraneus</em> trophozoites was suppressed, in contrast to <em>P. olseni</em>, which maintained their viability. Despite similar phagocytic indices for both species, the rate of phagosome acidification was significantly higher for hemocytes phagocytizing <em>P. mediterraneus</em> than those targeting <em>P. olseni</em>. Notably, phagosome acidification was significantly suppressed in hemocytes phagocytizing live <em>P. olseni</em> trophozoites, suggesting that <em>P. olseni</em> may secrete a substance that modulates phagosome acidification, and thereby evades intracellular digestion by the host’s hemocytes. Conversely, <em>P. mediterraneus</em>, with a lower affinity for infecting Manila clams, did not exhibit such modulation. Based on these results, we consider that the ability to modulate phagosome acidification in host hemocytes might be at least one factor in determining the host range of <em>Perkinsus</em> species.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Differences in phagosome acidification of Manila clam hemocytes in response to two Perkinsus species with contrasting proliferation dynamics in the host: P. olseni and P. mediterraneus\",\"authors\":\"Vince Neil B. Fuertes, Yuho Watanabe, Naoki Itoh\",\"doi\":\"10.1016/j.jip.2024.108216\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Understanding the factors determining the host ranges of <em>Perkinsus</em> spp., a significant group of pathogenic protozoans affecting shellfish, is essential for preventing their spread and designing effective control measures. Considering that differences in the ability to proliferate within the host may influence the determination of host range, we first injected six <em>Perkinsus</em> spp. into Manila clams <em>Ruditapes philippinarum</em> and monitored the variations of trophozoite numbers. Although all six species were detected in the challenged clams 28 days post infection, the infection intensities varied among species, and particularly two species showed contrasting infection trends: <em>P. mediterraneus</em> showed a decreasing trend of infection, declining to the lowest intensity, whereas that of <em>P. olseni</em> continuously increased, reaching the highest intensity. <em>In vitro</em> exposure to Manila clam hemocytes revealed that the survival of <em>P. mediterraneus</em> trophozoites was suppressed, in contrast to <em>P. olseni</em>, which maintained their viability. Despite similar phagocytic indices for both species, the rate of phagosome acidification was significantly higher for hemocytes phagocytizing <em>P. mediterraneus</em> than those targeting <em>P. olseni</em>. Notably, phagosome acidification was significantly suppressed in hemocytes phagocytizing live <em>P. olseni</em> trophozoites, suggesting that <em>P. olseni</em> may secrete a substance that modulates phagosome acidification, and thereby evades intracellular digestion by the host’s hemocytes. Conversely, <em>P. mediterraneus</em>, with a lower affinity for infecting Manila clams, did not exhibit such modulation. Based on these results, we consider that the ability to modulate phagosome acidification in host hemocytes might be at least one factor in determining the host range of <em>Perkinsus</em> species.</div></div>\",\"PeriodicalId\":16296,\"journal\":{\"name\":\"Journal of invertebrate pathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of invertebrate pathology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022201124001599\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of invertebrate pathology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022201124001599","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
Differences in phagosome acidification of Manila clam hemocytes in response to two Perkinsus species with contrasting proliferation dynamics in the host: P. olseni and P. mediterraneus
Understanding the factors determining the host ranges of Perkinsus spp., a significant group of pathogenic protozoans affecting shellfish, is essential for preventing their spread and designing effective control measures. Considering that differences in the ability to proliferate within the host may influence the determination of host range, we first injected six Perkinsus spp. into Manila clams Ruditapes philippinarum and monitored the variations of trophozoite numbers. Although all six species were detected in the challenged clams 28 days post infection, the infection intensities varied among species, and particularly two species showed contrasting infection trends: P. mediterraneus showed a decreasing trend of infection, declining to the lowest intensity, whereas that of P. olseni continuously increased, reaching the highest intensity. In vitro exposure to Manila clam hemocytes revealed that the survival of P. mediterraneus trophozoites was suppressed, in contrast to P. olseni, which maintained their viability. Despite similar phagocytic indices for both species, the rate of phagosome acidification was significantly higher for hemocytes phagocytizing P. mediterraneus than those targeting P. olseni. Notably, phagosome acidification was significantly suppressed in hemocytes phagocytizing live P. olseni trophozoites, suggesting that P. olseni may secrete a substance that modulates phagosome acidification, and thereby evades intracellular digestion by the host’s hemocytes. Conversely, P. mediterraneus, with a lower affinity for infecting Manila clams, did not exhibit such modulation. Based on these results, we consider that the ability to modulate phagosome acidification in host hemocytes might be at least one factor in determining the host range of Perkinsus species.
期刊介绍:
The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates.
The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.