Xuanzuo Tao , Yanxia Mao , Anguan Wang , Zhihong Zeng , Shaojun Zheng , Chunhui Jiang , Shu-Yang Chen , Hongfei Lu
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引用次数: 0
摘要
为识别草甘膦和甲磺隆，我们开发了一种带有希夫碱分子的嘌呤荧光探针。检测到的草甘膦和甲磺隆可导致希夫碱探针解离，从而通过关断 PET 过程增强荧光。机理研究表明，草甘膦中的磷酸和仲胺分子的协同作用导致席夫碱探针的键裂解。草甘膦和间三嗪的定量检测限分别为 17.2 nM 和 484.32 nM。同时，还在实际样品和细胞中进行了草甘膦农药的检测，证明该探针具有良好的实用性和细胞相容性。
A purine fluorescent derived probe assay for glyphosate and mesotrione via Schiff base cleavage
A fluorescent probe derived from purine with Schiff base moiety was developed for the recognization of glyphosate and mesotrione. The detected glyphosate and mesotrione can lead to the dissociation of the Schiff base probe to enhance the fluorescence via a turn-off PET process. Mechanism study revealed that the synergistic effect of the phosphoric acid and the secondary amine moieties in glyphosate results in the bond cleavage of the Schiff base probe. Quantitative measurements of glyphosate and mesotrione were achieved with the detection limits of 17.2 nM and 484.32 nM, respectively. Meanwhile, the detection of glyphosate pesticide in real samples and cells was also conducted, demonstrating the good practicality and cytocompatibility of the probe.
期刊介绍：
Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science.
The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments.
Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate.
Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to:
Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences,
Novel experimental techniques or instrumentation for molecular spectroscopy,
Novel theoretical and computational methods,
Novel applications in photochemistry and photobiology,
Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.
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