Yumin Hao, Zihan Wang, Huiping Wang, Wenjuan Dong, Yang Liu, Qin Hu, Shaomin Shuang, Chuan Dong, Yujing Guo, Xiaojuan Gong
{"title":"合理设计用于食品基质中亚硝酸盐比率测量双信号和智能手机辅助视觉检测的碳点纳米酶","authors":"Yumin Hao, Zihan Wang, Huiping Wang, Wenjuan Dong, Yang Liu, Qin Hu, Shaomin Shuang, Chuan Dong, Yujing Guo, Xiaojuan Gong","doi":"10.1016/j.jhazmat.2024.136156","DOIUrl":null,"url":null,"abstract":"Developing reliable nitrite (NO<sub>2</sub><sup>−</sup>) sensors is essential for food safety and reducing health risks from NO<sub>2</sub><sup>−</sup> exposure. In this study, we strategically designed nitrogen-doped carbon dot (N-CD) nanozymes to establish an accessible dual-signal ratiometric sensing system for detecting NO<sub>2</sub><sup>−</sup> in food matrices. This system utilizes the photoluminescence and enzyme-like properties of N-CD nanozymes combined with NO<sub>2</sub><sup>−</sup>-triggered diazotization reactions of substrates such as <em>o</em>-phenylenediamine (OPD) or 3,3',5,5'-tetramethylbenzidine (TMB). The resulting N-CD/OPD and N-CD/TMB composites provide dual-mode detection—fluorescence and colorimetric—with high selectivity for NO<sub>2</sub><sup>−</sup> and excellent resistance to interference. These sensors exhibit clear color changes under both ultraviolet and visible light, and can be combined with smartphones for visual, on-site detection of NO<sub>2</sub><sup>−</sup>. By incorporating a ratiometric strategy, dual-signal output, and smartphone compatibility, our system achieved a low detection limit (≤ 1.92<!-- --> <!-- -->μM) and satisfactory recovery rates (85.6~115%) in environmental water and food samples. This highlights the potential of smartphone-assisted sensors for environmental monitoring and food safety applications. Our carbon dot-based platform offers a practical and effective solution for on-site NO<sub>2</sub><sup>−</sup> detection, contributing valuable insights to the field.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":null,"pages":null},"PeriodicalIF":12.2000,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Rational design of carbon dot nanozymes for ratiometric dual-signal and smartphone-assisted visual detection of nitrite in food matrices\",\"authors\":\"Yumin Hao, Zihan Wang, Huiping Wang, Wenjuan Dong, Yang Liu, Qin Hu, Shaomin Shuang, Chuan Dong, Yujing Guo, Xiaojuan Gong\",\"doi\":\"10.1016/j.jhazmat.2024.136156\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Developing reliable nitrite (NO<sub>2</sub><sup>−</sup>) sensors is essential for food safety and reducing health risks from NO<sub>2</sub><sup>−</sup> exposure. In this study, we strategically designed nitrogen-doped carbon dot (N-CD) nanozymes to establish an accessible dual-signal ratiometric sensing system for detecting NO<sub>2</sub><sup>−</sup> in food matrices. This system utilizes the photoluminescence and enzyme-like properties of N-CD nanozymes combined with NO<sub>2</sub><sup>−</sup>-triggered diazotization reactions of substrates such as <em>o</em>-phenylenediamine (OPD) or 3,3',5,5'-tetramethylbenzidine (TMB). The resulting N-CD/OPD and N-CD/TMB composites provide dual-mode detection—fluorescence and colorimetric—with high selectivity for NO<sub>2</sub><sup>−</sup> and excellent resistance to interference. These sensors exhibit clear color changes under both ultraviolet and visible light, and can be combined with smartphones for visual, on-site detection of NO<sub>2</sub><sup>−</sup>. By incorporating a ratiometric strategy, dual-signal output, and smartphone compatibility, our system achieved a low detection limit (≤ 1.92<!-- --> <!-- -->μM) and satisfactory recovery rates (85.6~115%) in environmental water and food samples. This highlights the potential of smartphone-assisted sensors for environmental monitoring and food safety applications. Our carbon dot-based platform offers a practical and effective solution for on-site NO<sub>2</sub><sup>−</sup> detection, contributing valuable insights to the field.\",\"PeriodicalId\":361,\"journal\":{\"name\":\"Journal of Hazardous Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":12.2000,\"publicationDate\":\"2024-10-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Hazardous Materials\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jhazmat.2024.136156\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hazardous Materials","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.jhazmat.2024.136156","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Rational design of carbon dot nanozymes for ratiometric dual-signal and smartphone-assisted visual detection of nitrite in food matrices
Developing reliable nitrite (NO2−) sensors is essential for food safety and reducing health risks from NO2− exposure. In this study, we strategically designed nitrogen-doped carbon dot (N-CD) nanozymes to establish an accessible dual-signal ratiometric sensing system for detecting NO2− in food matrices. This system utilizes the photoluminescence and enzyme-like properties of N-CD nanozymes combined with NO2−-triggered diazotization reactions of substrates such as o-phenylenediamine (OPD) or 3,3',5,5'-tetramethylbenzidine (TMB). The resulting N-CD/OPD and N-CD/TMB composites provide dual-mode detection—fluorescence and colorimetric—with high selectivity for NO2− and excellent resistance to interference. These sensors exhibit clear color changes under both ultraviolet and visible light, and can be combined with smartphones for visual, on-site detection of NO2−. By incorporating a ratiometric strategy, dual-signal output, and smartphone compatibility, our system achieved a low detection limit (≤ 1.92 μM) and satisfactory recovery rates (85.6~115%) in environmental water and food samples. This highlights the potential of smartphone-assisted sensors for environmental monitoring and food safety applications. Our carbon dot-based platform offers a practical and effective solution for on-site NO2− detection, contributing valuable insights to the field.
期刊介绍:
The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.