{"title":"含有胡椒碱的瓜尔胶和羧甲基纤维素纳米复合材料:一种具有潜在抗菌和抗癌特性的多用途纳米粒子","authors":"Sanghita Das, Soumajit Chakrabarty, , Susmita Nandi, Debbethi Bera, Tarakdas Basu, Sukhen Das, Anindita Dey","doi":"10.1016/j.polymer.2024.127712","DOIUrl":null,"url":null,"abstract":"Guar gum-carboxymethyl cellulose derived piperine nanocomposite (GG-CMC@PIP) was synthesized to develop a phytochemical-based nanomaterial by following a simple synthetic procedure. In this study, piperine, an alkaloid prodrug was nanonized by entrapping within guar gum-carboxymethyl cellulose derived nanocomposite (GG-CMC). GG-CMC@PIPs were of (i) size (25 ± 3) nm by TEM, (ii) zeta potential (−) 33.1 ± .73 mV and (iii) entrapment efficiency 86 ± .46 %. High loading percentage of piperine in polymeric nanocomposites revealed excellent incorporation efficiency. In addition, pH responsive release behaviour of piperine from nanocomposites exhibited sustained but faster release pattern in pH 5.5. Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of piperine loaded nanocomposite on Gram positive <em>Enterococcus faecalis</em> and Gram-negative <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em> were in the range of 200–400 μg/ml. IC<sub>50</sub> value of GG-CMC@PIP was found to be around 500 μg/ml against C6 glioma cell line. Experimental outcome confirmed excessive accumulation of reactive oxygen species (ROS) mediated cytotoxicity, mitochondrial depolarization and higher degree of nuclear damage followed by increased mortality of C6 glioma cell after treatment with piperine-loaded nanocomposite.","PeriodicalId":405,"journal":{"name":"Polymer","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Guar gum and carboxymethyl cellulose nanocomposite loaded with piperine: a multipurpose nanoparticle with potential antibacterial and anticancer properties\",\"authors\":\"Sanghita Das, Soumajit Chakrabarty, , Susmita Nandi, Debbethi Bera, Tarakdas Basu, Sukhen Das, Anindita Dey\",\"doi\":\"10.1016/j.polymer.2024.127712\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Guar gum-carboxymethyl cellulose derived piperine nanocomposite (GG-CMC@PIP) was synthesized to develop a phytochemical-based nanomaterial by following a simple synthetic procedure. In this study, piperine, an alkaloid prodrug was nanonized by entrapping within guar gum-carboxymethyl cellulose derived nanocomposite (GG-CMC). GG-CMC@PIPs were of (i) size (25 ± 3) nm by TEM, (ii) zeta potential (−) 33.1 ± .73 mV and (iii) entrapment efficiency 86 ± .46 %. High loading percentage of piperine in polymeric nanocomposites revealed excellent incorporation efficiency. In addition, pH responsive release behaviour of piperine from nanocomposites exhibited sustained but faster release pattern in pH 5.5. Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of piperine loaded nanocomposite on Gram positive <em>Enterococcus faecalis</em> and Gram-negative <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em> were in the range of 200–400 μg/ml. IC<sub>50</sub> value of GG-CMC@PIP was found to be around 500 μg/ml against C6 glioma cell line. Experimental outcome confirmed excessive accumulation of reactive oxygen species (ROS) mediated cytotoxicity, mitochondrial depolarization and higher degree of nuclear damage followed by increased mortality of C6 glioma cell after treatment with piperine-loaded nanocomposite.\",\"PeriodicalId\":405,\"journal\":{\"name\":\"Polymer\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-10-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polymer\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.polymer.2024.127712\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"POLYMER SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polymer","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.polymer.2024.127712","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}
Guar gum and carboxymethyl cellulose nanocomposite loaded with piperine: a multipurpose nanoparticle with potential antibacterial and anticancer properties
Guar gum-carboxymethyl cellulose derived piperine nanocomposite (GG-CMC@PIP) was synthesized to develop a phytochemical-based nanomaterial by following a simple synthetic procedure. In this study, piperine, an alkaloid prodrug was nanonized by entrapping within guar gum-carboxymethyl cellulose derived nanocomposite (GG-CMC). GG-CMC@PIPs were of (i) size (25 ± 3) nm by TEM, (ii) zeta potential (−) 33.1 ± .73 mV and (iii) entrapment efficiency 86 ± .46 %. High loading percentage of piperine in polymeric nanocomposites revealed excellent incorporation efficiency. In addition, pH responsive release behaviour of piperine from nanocomposites exhibited sustained but faster release pattern in pH 5.5. Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of piperine loaded nanocomposite on Gram positive Enterococcus faecalis and Gram-negative Escherichia coli, Pseudomonas aeruginosa were in the range of 200–400 μg/ml. IC50 value of GG-CMC@PIP was found to be around 500 μg/ml against C6 glioma cell line. Experimental outcome confirmed excessive accumulation of reactive oxygen species (ROS) mediated cytotoxicity, mitochondrial depolarization and higher degree of nuclear damage followed by increased mortality of C6 glioma cell after treatment with piperine-loaded nanocomposite.
期刊介绍:
Polymer is an interdisciplinary journal dedicated to publishing innovative and significant advances in Polymer Physics, Chemistry and Technology. We welcome submissions on polymer hybrids, nanocomposites, characterisation and self-assembly. Polymer also publishes work on the technological application of polymers in energy and optoelectronics.
The main scope is covered but not limited to the following core areas:
Polymer Materials
Nanocomposites and hybrid nanomaterials
Polymer blends, films, fibres, networks and porous materials
Physical Characterization
Characterisation, modelling and simulation* of molecular and materials properties in bulk, solution, and thin films
Polymer Engineering
Advanced multiscale processing methods
Polymer Synthesis, Modification and Self-assembly
Including designer polymer architectures, mechanisms and kinetics, and supramolecular polymerization
Technological Applications
Polymers for energy generation and storage
Polymer membranes for separation technology
Polymers for opto- and microelectronics.