正常牦牛和隐睾牦牛垂体端细胞的形态鉴定和分布比较

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES BMC Veterinary Research Pub Date : 2024-10-11 DOI:10.1186/s12917-024-04307-1
Yumei Qi, Ligang Yuan, Jianlin Zeng, Xiaofen Wang, Long Ma, Jinghan Lv
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引用次数: 0

摘要

背景:端细胞(TCs)是哺乳动物许多器官中的一种新型间质细胞,参与组织代谢、机械支持、免疫调节等方面。本研究旨在探讨隐睾牦牛垂体TCs的组织化学特征及其变化:方法:通过透射电子显微镜(TEM)、甲苯胺蓝染色、免疫荧光、qRT-PCR和Western印迹等方法了解TCs的分布特征和生物学功能:结果:TEM证实垂体中存在TCs,它们具有小体和单形端粒(Tps)。Tps从细胞体延伸到窦周血管间隙,Tps的数量与细胞核的形态密切相关。隐睾牦牛垂体TC最明显的变化是Tps相对变短,分泌泡减少。H.E.和甲苯胺蓝染色显示,TCs不仅分布在窦状血管和腺细胞簇之间,还存在于血管内皮细胞表面。免疫荧光评估了TCs生物标记物的共表达,如Vimentin/CD34、CD117/CD34和α-SMA/CD34,以进一步确定TCs的表型特征。此外,我们还分析了这些生物标志物的 mRNA 和蛋白表达,以确定 TCs 的变化特征和可能的生物学作用。综上所述,本研究首次报道了牦牛垂体中TCs的生物学特征。虽然分布特征没有明显变化,但隐睾牦牛TCs生物学特征的变化非常明显,表明TCs参与了垂体局部微环境的改变。因此,我们的研究为进一步研究TCs在牦牛隐睾发生过程中在垂体中的作用提供了线索。
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Morphological identification and distribution comparison of telocytes in pituitary gland between normal and cryptorchid yaks.

Background: Telocytes (TCs) is a novel type of interstitial cells in many mammals organs, which participate in the organizational metabolism, mechanical support, immunomodulation and other aspects. The aim of this study was to explore the organizational chemical characteristics of TCs in pituitary gland and their changes in cryptorchid yaks.

Methods: Transmission electron microscopy (TEM), toluidine blue staining, immunofluorescence, qRT-PCR, and Western blotting may enable us to understand TCs distribution characteristics and biological functions.

Result: TEM confirmed the presence of TCs in the pituitary gland with small bodies and moniliform telopodes (Tps). The Tps extending out from the cell body to the peri-sinusoidal vessels spaces, the number of Tps is closely related to the morphology of the nucleus. The most obvious changes of TCs in the pituitary gland of cryptorchid yaks is the Tps are relatively shorter and decreased secretory vesicles. H.E. and toluidine blue staining revealed that TCs not only distributed between the sinusoidal blood vessels and the glandular cell clusters, but also present on the surface of vascular endothelial cells. The co-expression of TCs biomarkers, such as Vimentin/CD34, CD117/CD34 and α-SMA/CD34, were evaluated by immunofluorescence to further determine the phenotypic characteristics of TCs. Besides, we analyzed the mRNA and protein expression of these biomarkers to determine the characteristics of TCs changes and possible biological roles. Both the mRNA and protein expression of CD117 were significantly higher in the pituitary gland of cryptorchid yaks than in the normal (p < 0.01), the protein expression of CD34 in the cryptorchid yaks was significantly higher than the normal (p < 0.01). There were no significant difference in mRNA expression of Vimentin and α-SMA (p>0.05), while the protein expression were significantly increased in the normal yaks (p < 0.05).

Conclusions: In summary, this study reports for the first time that the biological characteristics of TCs in yak pituitary gland. Although there is no significant change in the distribution characteristics, the changes in biological features of TCs in cryptorchid yaks are clear, suggesting that TCs participated in alteration in the local microenvironment of the pituitary gland. Therefore, our study provides clues for further investigating the role of TCs in the pituitary gland during the occurrence of cryptorchidism in yaks.

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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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