Mélanie Tchoumbou, Tatjana Iezhova, Carolina Hernández-Lara, Mélanie Duc, Gediminas Valkiūnas
{"title":"揭示血孢子虫寄生虫(血孢子虫科、血孢子虫属)的外红细胞发育模式,以及自然感染鸟类的组织阶段流产案例。","authors":"Mélanie Tchoumbou, Tatjana Iezhova, Carolina Hernández-Lara, Mélanie Duc, Gediminas Valkiūnas","doi":"10.1016/j.ijpara.2024.10.002","DOIUrl":null,"url":null,"abstract":"<p><p>Haemoproteus species (Haemosporida, Haemoproteidae) are cosmopolitan blood parasites that affect bird fitness and health. Recent discoveries based on the application of molecular markers showed that exo-erythrocytic or tissue stages of haemoproteids damage various internal organs including the brain. However, the patterns of exo-erythrocytic development remain unclear for most of the described species. This study aimed to understand the exo-erythrocytic development of Haemoproteus parasites in naturally infected Thrush nightingales Luscinia luscinia (Muscicapidae). Infections were confirmed in eight bird individuals by microscopic examination and PCR-based methods. Organs were examined using histology and in situ hybridization, which applied genus-specific and lineage-specific oligonucleotide probes targeting the 18S rRNA of the parasites. Exo-erythrocytic meronts of Haemoproteus attenuatus (lineage hROBIN1) were found and described for the first known time in this avian host. Most meronts were seen in the lungs, with a few also present in the liver, heart, and pectoral muscle. The available data suggest that this parasite produces only meronts, and not megalomeronts. However, numerous megalomeronts at different stages of development were observed in the gizzard and the heart of one individual. Based on the morphology, location in organs, and diagnostics using the lineage-specific probes, the megalomeronts were attributed to Haemoproteus majoris (lineage hWW2). Two cases of empty capsular-like walls of megalomeronts were seen in the gizzard, indicating that the megalomeronts had already ruptured and degenerated. The extensive microscopic examination did not reveal gametocytes of H. majoris, obviously indicating an abortive development. Abortive haemosporidian infections were often speculated to occur in wildlife but have not been documented in naturally infected birds. This study recognised patterns in the exo-erythrocytic development of H. attenuatus, and is to our knowledge the first documentation of abortive Haemoproteus infection in a naturally infected bird during exo-erythrocytic development.</p>","PeriodicalId":13725,"journal":{"name":"International journal for parasitology","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unravelling the patterns of exo-erythrocytic development of Haemoproteus parasites (Haemoproteidae, Haemosporida), with a case of abortive tissue stages in a naturally infected bird.\",\"authors\":\"Mélanie Tchoumbou, Tatjana Iezhova, Carolina Hernández-Lara, Mélanie Duc, Gediminas Valkiūnas\",\"doi\":\"10.1016/j.ijpara.2024.10.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Haemoproteus species (Haemosporida, Haemoproteidae) are cosmopolitan blood parasites that affect bird fitness and health. Recent discoveries based on the application of molecular markers showed that exo-erythrocytic or tissue stages of haemoproteids damage various internal organs including the brain. However, the patterns of exo-erythrocytic development remain unclear for most of the described species. This study aimed to understand the exo-erythrocytic development of Haemoproteus parasites in naturally infected Thrush nightingales Luscinia luscinia (Muscicapidae). Infections were confirmed in eight bird individuals by microscopic examination and PCR-based methods. Organs were examined using histology and in situ hybridization, which applied genus-specific and lineage-specific oligonucleotide probes targeting the 18S rRNA of the parasites. Exo-erythrocytic meronts of Haemoproteus attenuatus (lineage hROBIN1) were found and described for the first known time in this avian host. Most meronts were seen in the lungs, with a few also present in the liver, heart, and pectoral muscle. The available data suggest that this parasite produces only meronts, and not megalomeronts. However, numerous megalomeronts at different stages of development were observed in the gizzard and the heart of one individual. Based on the morphology, location in organs, and diagnostics using the lineage-specific probes, the megalomeronts were attributed to Haemoproteus majoris (lineage hWW2). Two cases of empty capsular-like walls of megalomeronts were seen in the gizzard, indicating that the megalomeronts had already ruptured and degenerated. The extensive microscopic examination did not reveal gametocytes of H. majoris, obviously indicating an abortive development. Abortive haemosporidian infections were often speculated to occur in wildlife but have not been documented in naturally infected birds. This study recognised patterns in the exo-erythrocytic development of H. attenuatus, and is to our knowledge the first documentation of abortive Haemoproteus infection in a naturally infected bird during exo-erythrocytic development.</p>\",\"PeriodicalId\":13725,\"journal\":{\"name\":\"International journal for parasitology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal for parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.ijpara.2024.10.002\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal for parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.ijpara.2024.10.002","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Unravelling the patterns of exo-erythrocytic development of Haemoproteus parasites (Haemoproteidae, Haemosporida), with a case of abortive tissue stages in a naturally infected bird.
Haemoproteus species (Haemosporida, Haemoproteidae) are cosmopolitan blood parasites that affect bird fitness and health. Recent discoveries based on the application of molecular markers showed that exo-erythrocytic or tissue stages of haemoproteids damage various internal organs including the brain. However, the patterns of exo-erythrocytic development remain unclear for most of the described species. This study aimed to understand the exo-erythrocytic development of Haemoproteus parasites in naturally infected Thrush nightingales Luscinia luscinia (Muscicapidae). Infections were confirmed in eight bird individuals by microscopic examination and PCR-based methods. Organs were examined using histology and in situ hybridization, which applied genus-specific and lineage-specific oligonucleotide probes targeting the 18S rRNA of the parasites. Exo-erythrocytic meronts of Haemoproteus attenuatus (lineage hROBIN1) were found and described for the first known time in this avian host. Most meronts were seen in the lungs, with a few also present in the liver, heart, and pectoral muscle. The available data suggest that this parasite produces only meronts, and not megalomeronts. However, numerous megalomeronts at different stages of development were observed in the gizzard and the heart of one individual. Based on the morphology, location in organs, and diagnostics using the lineage-specific probes, the megalomeronts were attributed to Haemoproteus majoris (lineage hWW2). Two cases of empty capsular-like walls of megalomeronts were seen in the gizzard, indicating that the megalomeronts had already ruptured and degenerated. The extensive microscopic examination did not reveal gametocytes of H. majoris, obviously indicating an abortive development. Abortive haemosporidian infections were often speculated to occur in wildlife but have not been documented in naturally infected birds. This study recognised patterns in the exo-erythrocytic development of H. attenuatus, and is to our knowledge the first documentation of abortive Haemoproteus infection in a naturally infected bird during exo-erythrocytic development.
期刊介绍:
International Journal for Parasitology offers authors the option to sponsor nonsubscriber access to their articles on Elsevier electronic publishing platforms. For more information please view our Sponsored Articles page. The International Journal for Parasitology publishes the results of original research in all aspects of basic and applied parasitology, including all the fields covered by its Specialist Editors, and ranging from parasites and host-parasite relationships of intrinsic biological interest to those of social and economic importance in human and veterinary medicine and agriculture.