Najmeh Zare, Hassan Karimi-Maleh, Zhouxiang Zhang, Yangpin Wen, Nianbing Zhong, Li Fu
{"title":"利用 TiVC MXenes/金纳米复合材料放大分析生物传感器增强对 HER2 的检测，实现对癌症生物标记物的精确监测","authors":"Najmeh Zare, Hassan Karimi-Maleh, Zhouxiang Zhang, Yangpin Wen, Nianbing Zhong, Li Fu","doi":"10.1007/s42114-024-00966-8","DOIUrl":null,"url":null,"abstract":"<div><p>Recently, aptamers have been widely used in the detection and measurement of cancer biomarkers. This issue has had a significant impact on the process of diagnosing all types of cancers. This research work explores the development and application of layer-by-layer modified electrochemical apta-sensor for the precise monitoring of HER2, a crucial biomarker associated with breast cancer. The surface of the screen-printed carbon electrode was modified with gold nanoparticle (Au-NP) and TiVC MXene catalyst plus Pb<sup>2+</sup> loaded aptamer (SPCE/TiVC-MXene/Au NPs/Pb<sup>2+</sup>-aptamer), which showed a high selectivity and affinity towards HER2 and offered a sensitive detection platform. The MXene nano-layer was synthesized and characterized by XPS, MAP, EDS, AFM, BET, and TEM methods and used as a substrate to improve electrochemical conductivity and loading of biological recognition element. The square-wave anodic stripping voltammetry (SWASV) method was used as a highly sensitive platform in HER2 detection. The difference of stripping signals of the Pb<sup>2+</sup> from the SPCE/TiVC-MXene/Au NPs/Pb<sup>2+</sup>-aptamer before and after incubation in HER2 solution was selected as analytical response to achieve a reliable and quantitative analysis for HER2 concentrations. The effective factors in monitoring of HER2 such as concentration of Pb<sup>2+</sup>, incubation time, and buffer type were optimized and results showed that 5 mM of Pb<sup>2+</sup> and 90-min incubation time in Tris–HCl created best condition in fabrication of biosensor. The results demonstrate a linear dynamic range of 1.0–1200 pg/mL for monitoring of HER2 with limit of detection of 50 fg/mL. A good affinity of fabricated apta-sensor to HER2 in the presence some other biomarkers such as PR, ER, and CEA confirmed the selectivity of the fabricated biosensor towards HER2 detection.</p></div>","PeriodicalId":7220,"journal":{"name":"Advanced Composites and Hybrid Materials","volume":null,"pages":null},"PeriodicalIF":23.2000,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced detection of HER2 using a TiVC MXenes/gold nanocomposite amplified analytical biosensor for precise cancer biomarker monitoring\",\"authors\":\"Najmeh Zare, Hassan Karimi-Maleh, Zhouxiang Zhang, Yangpin Wen, Nianbing Zhong, Li Fu\",\"doi\":\"10.1007/s42114-024-00966-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Recently, aptamers have been widely used in the detection and measurement of cancer biomarkers. This issue has had a significant impact on the process of diagnosing all types of cancers. This research work explores the development and application of layer-by-layer modified electrochemical apta-sensor for the precise monitoring of HER2, a crucial biomarker associated with breast cancer. The surface of the screen-printed carbon electrode was modified with gold nanoparticle (Au-NP) and TiVC MXene catalyst plus Pb<sup>2+</sup> loaded aptamer (SPCE/TiVC-MXene/Au NPs/Pb<sup>2+</sup>-aptamer), which showed a high selectivity and affinity towards HER2 and offered a sensitive detection platform. The MXene nano-layer was synthesized and characterized by XPS, MAP, EDS, AFM, BET, and TEM methods and used as a substrate to improve electrochemical conductivity and loading of biological recognition element. The square-wave anodic stripping voltammetry (SWASV) method was used as a highly sensitive platform in HER2 detection. The difference of stripping signals of the Pb<sup>2+</sup> from the SPCE/TiVC-MXene/Au NPs/Pb<sup>2+</sup>-aptamer before and after incubation in HER2 solution was selected as analytical response to achieve a reliable and quantitative analysis for HER2 concentrations. The effective factors in monitoring of HER2 such as concentration of Pb<sup>2+</sup>, incubation time, and buffer type were optimized and results showed that 5 mM of Pb<sup>2+</sup> and 90-min incubation time in Tris–HCl created best condition in fabrication of biosensor. The results demonstrate a linear dynamic range of 1.0–1200 pg/mL for monitoring of HER2 with limit of detection of 50 fg/mL. A good affinity of fabricated apta-sensor to HER2 in the presence some other biomarkers such as PR, ER, and CEA confirmed the selectivity of the fabricated biosensor towards HER2 detection.</p></div>\",\"PeriodicalId\":7220,\"journal\":{\"name\":\"Advanced Composites and Hybrid Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":23.2000,\"publicationDate\":\"2024-10-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced Composites and Hybrid Materials\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s42114-024-00966-8\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, COMPOSITES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Composites and Hybrid Materials","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s42114-024-00966-8","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, COMPOSITES","Score":null,"Total":0}
Enhanced detection of HER2 using a TiVC MXenes/gold nanocomposite amplified analytical biosensor for precise cancer biomarker monitoring
Recently, aptamers have been widely used in the detection and measurement of cancer biomarkers. This issue has had a significant impact on the process of diagnosing all types of cancers. This research work explores the development and application of layer-by-layer modified electrochemical apta-sensor for the precise monitoring of HER2, a crucial biomarker associated with breast cancer. The surface of the screen-printed carbon electrode was modified with gold nanoparticle (Au-NP) and TiVC MXene catalyst plus Pb2+ loaded aptamer (SPCE/TiVC-MXene/Au NPs/Pb2+-aptamer), which showed a high selectivity and affinity towards HER2 and offered a sensitive detection platform. The MXene nano-layer was synthesized and characterized by XPS, MAP, EDS, AFM, BET, and TEM methods and used as a substrate to improve electrochemical conductivity and loading of biological recognition element. The square-wave anodic stripping voltammetry (SWASV) method was used as a highly sensitive platform in HER2 detection. The difference of stripping signals of the Pb2+ from the SPCE/TiVC-MXene/Au NPs/Pb2+-aptamer before and after incubation in HER2 solution was selected as analytical response to achieve a reliable and quantitative analysis for HER2 concentrations. The effective factors in monitoring of HER2 such as concentration of Pb2+, incubation time, and buffer type were optimized and results showed that 5 mM of Pb2+ and 90-min incubation time in Tris–HCl created best condition in fabrication of biosensor. The results demonstrate a linear dynamic range of 1.0–1200 pg/mL for monitoring of HER2 with limit of detection of 50 fg/mL. A good affinity of fabricated apta-sensor to HER2 in the presence some other biomarkers such as PR, ER, and CEA confirmed the selectivity of the fabricated biosensor towards HER2 detection.
期刊介绍：
Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field.
The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest.
Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials.
Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
