N. P. Antonova, I. V. Grigoriev, A. M. Lendel, O. V. Usacheva, A. A. Klimova, E. V. Usachev, V. A. Gushchin, D. V. Vasina
{"title":"对重组内溶解素 LysSi3 进行工程改造以增强其抗菌特性","authors":"N. P. Antonova, I. V. Grigoriev, A. M. Lendel, O. V. Usacheva, A. A. Klimova, E. V. Usachev, V. A. Gushchin, D. V. Vasina","doi":"10.1134/S000368382460492X","DOIUrl":null,"url":null,"abstract":"<p>The potential of new genetically modified recombinant endolysins as antimicrobial agents against Gram-negative bacteria was investigated. A series of enzymes based on LysSi3 lysozyme-like muramidase was obtained by modifying its sequence with antimicrobial peptides of different families, and recombinant expression in <i>E. coli</i> was demonstrated. Modification of LysSi3 resulted in increased bacteriolytic activity against the model isolate of <i>A. baumannii</i> and a higher kinetics rate compared to the native enzyme. The cytotoxic properties of the newly engineered lysins were investigated with the HEK293 and HaCaT cell lines, and it was shown that modification of LysSi3 with antimicrobial peptides does not significantly increase the toxic properties in vitro.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 5","pages":"802 - 811"},"PeriodicalIF":1.0000,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S000368382460492X.pdf","citationCount":"0","resultStr":"{\"title\":\"Engineering of Recombinant Endolysin LysSi3 to Increase its Antibacterial Properties\",\"authors\":\"N. P. Antonova, I. V. Grigoriev, A. M. Lendel, O. V. Usacheva, A. A. Klimova, E. V. Usachev, V. A. Gushchin, D. V. Vasina\",\"doi\":\"10.1134/S000368382460492X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The potential of new genetically modified recombinant endolysins as antimicrobial agents against Gram-negative bacteria was investigated. A series of enzymes based on LysSi3 lysozyme-like muramidase was obtained by modifying its sequence with antimicrobial peptides of different families, and recombinant expression in <i>E. coli</i> was demonstrated. Modification of LysSi3 resulted in increased bacteriolytic activity against the model isolate of <i>A. baumannii</i> and a higher kinetics rate compared to the native enzyme. The cytotoxic properties of the newly engineered lysins were investigated with the HEK293 and HaCaT cell lines, and it was shown that modification of LysSi3 with antimicrobial peptides does not significantly increase the toxic properties in vitro.</p>\",\"PeriodicalId\":466,\"journal\":{\"name\":\"Applied Biochemistry and Microbiology\",\"volume\":\"60 5\",\"pages\":\"802 - 811\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1134/S000368382460492X.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Biochemistry and Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S000368382460492X\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S000368382460492X","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Engineering of Recombinant Endolysin LysSi3 to Increase its Antibacterial Properties
The potential of new genetically modified recombinant endolysins as antimicrobial agents against Gram-negative bacteria was investigated. A series of enzymes based on LysSi3 lysozyme-like muramidase was obtained by modifying its sequence with antimicrobial peptides of different families, and recombinant expression in E. coli was demonstrated. Modification of LysSi3 resulted in increased bacteriolytic activity against the model isolate of A. baumannii and a higher kinetics rate compared to the native enzyme. The cytotoxic properties of the newly engineered lysins were investigated with the HEK293 and HaCaT cell lines, and it was shown that modification of LysSi3 with antimicrobial peptides does not significantly increase the toxic properties in vitro.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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