K. B. Shumaev, D. I. Grachev, O. V. Kosmachevskaya, A. F. Topunov, E. K. Ruuge
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引用次数: 0
摘要
安杰利盐是一种硝基供体,因此可以防止溶血的负面影响,如血浆中游离血红蛋白的血管收缩效应。然而,亚硝基与各种血蛋白相互作用的分子机制尚未得到充分阐明。众所周知,氧化应激产生的血蛋白中的氧代铁氧体是一种强促氧化剂。在本研究中,研究了血红蛋白和肌红蛋白的甲基和氧杂环戊基与亚硝基的还原亚硝基化作用。实验在体外进行,利用电子顺磁共振光谱检测血红蛋白的亚硝基形式。结果表明,在模拟血红蛋白或肌红蛋白被过氧化氢氧化的系统中,安杰利盐具有抗氧化作用。此外,在血红蛋白和肌红蛋白元形式中加入过氧化氢会导致出现自由基的 EPR 信号,其 g = 2.005 与这些血蛋白的蛋白质部分有关。因此,硝基同时起到了还原剂和亚硝基化剂的作用,从而阻止了血红蛋白中氧代铁酰形式的形成。安杰利盐的治疗特性可能在很大程度上与它对血液成分的抗氧化作用有关。
Reductive Nitrosylation of Hemoglobin and Myoglobin and its Antioxidant Effect
Angeli’s salt is a nitroxyl donor, due to which it can prevent the negative consequences of hemolysis, such as the vasoconstrictive effect of free hemoglobin in blood plasma. However, the molecular mechanisms of the interaction of nitroxyl with various hemoproteins have not been sufficiently elucidated. It is known that oxoferryl forms of hemoproteins arising from oxidative stress are strong prooxidants. In this study, the reductive nitrosylation of meth- and oxoferryl forms of hemoglobin and myoglobin with nitroxyl was investigated. The experiments were carried out in vitro by detecting nitrosyl forms of hemoproteins using electron paramagnetic resonance spectroscopy. The results indicate the antioxidant effect of Angeli’s salt in systems simulating the oxidation of hemoglobin or myoglobin by hydrogen peroxide. As well, the addition of hydrogen peroxide to hemoglobin and myoglobin metforms led to the appearance of an EPR signal of free radicals with g = 2.005 associated with the protein part of these hemoproteins. Thus, nitroxyl acted simultaneously as a reducing and nitrosylating agent, thereby preventing the formation of oxoferryl forms of hemoproteins. The therapeutic properties of Angeli’s salt may be largely related to the antioxidant effect exerted on blood components.
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍:
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.