João Pereira Santos , David Amouroux , Vera I. Slaveykova
{"title":"淡水浮游植物物种的溶解元素汞积累:试点研究","authors":"João Pereira Santos , David Amouroux , Vera I. Slaveykova","doi":"10.1016/j.hazl.2024.100128","DOIUrl":null,"url":null,"abstract":"<div><div>Bioaccumulation of dissolved elemental mercury (DGM) by various organisms has been demonstrated, but no study has shown its uptake and sequestration by phytoplankton species. The present study aims to investigate the accumulation of mercury by phytoplankton species exposed to DGM. Diatoms (<em>Cyclotella meneghiniana</em> and <em>Navicula pelliculosa</em>) and green algae (<em>Chlamydomonas reinhardtii</em> and <em>Haematococcus pluvialis</em>) were exposed to constant high level of atmospheric gaseous Hg (∼7.7 µg m<sup>−3</sup>). Total mercury concentrations (THg) in the medium (dissolved fraction) and algae cells (cellular fraction) were determined using cold vapor atomic fluorescence spectroscopy. Results revealed a partitioning of Hg(0) between the atmosphere and phytoplankton cultures, with THg predominantly found in the algae cells. THg in the algae cultures decreased in the order: <em>C. reinhardtii</em> > <em>H. pluvialis</em> > <em>N. pelliculosa</em> > <em>C. meneghiniana</em>. However, the cellular concentration (mol cell<sup>−1</sup>) decreased in the order: <em>H. pluvialis</em> > <em>C. reinhardtii</em> > <em>C. meneghiniana</em> > <em>N. pelliculosa.</em> These results highlight species specificity in Hg accumulation upon exposure to DGM, further linked to the phytoplankton surface area. Our findings reveal for the first time that phytoplankton species significantly influence the partitioning of atmospheric Hg(0) in aquatic environments, with important implications for the understanding of the aquatic mercury cycle.</div></div>","PeriodicalId":93463,"journal":{"name":"Journal of hazardous materials letters","volume":"5 ","pages":"Article 100128"},"PeriodicalIF":6.6000,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dissolved elemental mercury accumulation by freshwater phytoplankton species: A pilot study\",\"authors\":\"João Pereira Santos , David Amouroux , Vera I. Slaveykova\",\"doi\":\"10.1016/j.hazl.2024.100128\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Bioaccumulation of dissolved elemental mercury (DGM) by various organisms has been demonstrated, but no study has shown its uptake and sequestration by phytoplankton species. The present study aims to investigate the accumulation of mercury by phytoplankton species exposed to DGM. Diatoms (<em>Cyclotella meneghiniana</em> and <em>Navicula pelliculosa</em>) and green algae (<em>Chlamydomonas reinhardtii</em> and <em>Haematococcus pluvialis</em>) were exposed to constant high level of atmospheric gaseous Hg (∼7.7 µg m<sup>−3</sup>). Total mercury concentrations (THg) in the medium (dissolved fraction) and algae cells (cellular fraction) were determined using cold vapor atomic fluorescence spectroscopy. Results revealed a partitioning of Hg(0) between the atmosphere and phytoplankton cultures, with THg predominantly found in the algae cells. THg in the algae cultures decreased in the order: <em>C. reinhardtii</em> > <em>H. pluvialis</em> > <em>N. pelliculosa</em> > <em>C. meneghiniana</em>. However, the cellular concentration (mol cell<sup>−1</sup>) decreased in the order: <em>H. pluvialis</em> > <em>C. reinhardtii</em> > <em>C. meneghiniana</em> > <em>N. pelliculosa.</em> These results highlight species specificity in Hg accumulation upon exposure to DGM, further linked to the phytoplankton surface area. Our findings reveal for the first time that phytoplankton species significantly influence the partitioning of atmospheric Hg(0) in aquatic environments, with important implications for the understanding of the aquatic mercury cycle.</div></div>\",\"PeriodicalId\":93463,\"journal\":{\"name\":\"Journal of hazardous materials letters\",\"volume\":\"5 \",\"pages\":\"Article 100128\"},\"PeriodicalIF\":6.6000,\"publicationDate\":\"2024-10-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of hazardous materials letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666911024000273\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of hazardous materials letters","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666911024000273","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
引用次数: 0
摘要
各种生物对溶解元素汞(DGM)的生物累积已得到证实,但还没有研究显示浮游植物物种对其的吸收和固存。本研究旨在调查暴露于 DGM 的浮游植物物种的汞积累情况。硅藻(Cyclotella meneghiniana 和 Navicula pelliculosa)和绿藻(Chlamydomonas reinhardtii 和 Haematococcus pluvialis)持续暴露于高浓度大气气态汞(∼7.7 µg m-3)中。采用冷蒸气原子荧光光谱法测定了培养基(溶解部分)和藻细胞(细胞部分)中的总汞浓度(THg)。结果显示,Hg(0) 在大气和浮游植物培养物之间分配,THg 主要存在于藻类细胞中。藻类培养物中的 THg 依次减少:C. reinhardtii > H. pluvialis > N. pelliculosa > C. meneghiniana。然而,细胞浓度(mol cell-1)依次下降:H. pluvialis > C. reinhardtii > C. meneghiniana > N. pelliculosa。这些结果突显了暴露于 DGM 后汞积累的物种特异性,并与浮游植物的表面积进一步相关。我们的研究结果首次揭示了浮游植物物种对水生环境中大气汞分配的重要影响,这对了解水生汞循环具有重要意义。
Dissolved elemental mercury accumulation by freshwater phytoplankton species: A pilot study
Bioaccumulation of dissolved elemental mercury (DGM) by various organisms has been demonstrated, but no study has shown its uptake and sequestration by phytoplankton species. The present study aims to investigate the accumulation of mercury by phytoplankton species exposed to DGM. Diatoms (Cyclotella meneghiniana and Navicula pelliculosa) and green algae (Chlamydomonas reinhardtii and Haematococcus pluvialis) were exposed to constant high level of atmospheric gaseous Hg (∼7.7 µg m−3). Total mercury concentrations (THg) in the medium (dissolved fraction) and algae cells (cellular fraction) were determined using cold vapor atomic fluorescence spectroscopy. Results revealed a partitioning of Hg(0) between the atmosphere and phytoplankton cultures, with THg predominantly found in the algae cells. THg in the algae cultures decreased in the order: C. reinhardtii > H. pluvialis > N. pelliculosa > C. meneghiniana. However, the cellular concentration (mol cell−1) decreased in the order: H. pluvialis > C. reinhardtii > C. meneghiniana > N. pelliculosa. These results highlight species specificity in Hg accumulation upon exposure to DGM, further linked to the phytoplankton surface area. Our findings reveal for the first time that phytoplankton species significantly influence the partitioning of atmospheric Hg(0) in aquatic environments, with important implications for the understanding of the aquatic mercury cycle.