María B. Ballatore , Javier E. Durantini , Claudia Solis , Milena B. Boarini , Miguel Gervaldo , Luis Otero , María E. Milanesio , Edgardo N. Durantini
{"title":"涂有卟啉衍生聚合物的光动力表面可消除金黄色葡萄球菌生物膜","authors":"María B. Ballatore , Javier E. Durantini , Claudia Solis , Milena B. Boarini , Miguel Gervaldo , Luis Otero , María E. Milanesio , Edgardo N. Durantini","doi":"10.1016/j.jphotochem.2024.116083","DOIUrl":null,"url":null,"abstract":"<div><div>Zn(II) porphyrin derivatives (ZnACP and ZnTCP-C<sub>60</sub>) were used to obtain electrogenerated polymeric films on optically transparent indium tin oxide (ITO) electrodes. This approach produced stable and reproducible polymers that increase the lipophilicity of the surfaces. The spectroscopic and photodynamic properties of these polymeric films were compared with those corresponding to their porphyrin units. These materials photosensitized singlet molecular oxygen in water. Photoinactivation activity of the films were investigated in <em>Staphylococcus aureus</em> biofilms depositing on the surfaces. Thus, the ZnTCP-C<sub>60</sub> film was effective in reducing bacterial survival, reaching a photokilling of 99.7 %. In addition, ZnACP-coated surface with an absorption of 0.7 at Soret band produced a notable decrease of 4 log units in cell survival (equivalent to 99.99 % reduction) upon an exposure to white light for 60 min. Confocal scanning microscopy and scanning electron microscopy were employed to detect morphological alterations. In addition to its bactericidal effects within the biofilm, PDI using ZnACP films induced cell detachment, thereby disrupting the overall architecture of the biofilm. Thus, these polymeric films emerge as compelling photodynamic active surfaces for eradicating <em>S. aureus</em> bacterial biofilms.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"459 ","pages":"Article 116083"},"PeriodicalIF":4.1000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Photodynamic surfaces coated with porphyrin-derived polymers to eradicate Staphylococcus aureus biofilms\",\"authors\":\"María B. Ballatore , Javier E. Durantini , Claudia Solis , Milena B. Boarini , Miguel Gervaldo , Luis Otero , María E. Milanesio , Edgardo N. Durantini\",\"doi\":\"10.1016/j.jphotochem.2024.116083\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Zn(II) porphyrin derivatives (ZnACP and ZnTCP-C<sub>60</sub>) were used to obtain electrogenerated polymeric films on optically transparent indium tin oxide (ITO) electrodes. This approach produced stable and reproducible polymers that increase the lipophilicity of the surfaces. The spectroscopic and photodynamic properties of these polymeric films were compared with those corresponding to their porphyrin units. These materials photosensitized singlet molecular oxygen in water. Photoinactivation activity of the films were investigated in <em>Staphylococcus aureus</em> biofilms depositing on the surfaces. Thus, the ZnTCP-C<sub>60</sub> film was effective in reducing bacterial survival, reaching a photokilling of 99.7 %. In addition, ZnACP-coated surface with an absorption of 0.7 at Soret band produced a notable decrease of 4 log units in cell survival (equivalent to 99.99 % reduction) upon an exposure to white light for 60 min. Confocal scanning microscopy and scanning electron microscopy were employed to detect morphological alterations. In addition to its bactericidal effects within the biofilm, PDI using ZnACP films induced cell detachment, thereby disrupting the overall architecture of the biofilm. Thus, these polymeric films emerge as compelling photodynamic active surfaces for eradicating <em>S. aureus</em> bacterial biofilms.</div></div>\",\"PeriodicalId\":16782,\"journal\":{\"name\":\"Journal of Photochemistry and Photobiology A-chemistry\",\"volume\":\"459 \",\"pages\":\"Article 116083\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Photochemistry and Photobiology A-chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1010603024006270\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Photochemistry and Photobiology A-chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1010603024006270","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Photodynamic surfaces coated with porphyrin-derived polymers to eradicate Staphylococcus aureus biofilms
Zn(II) porphyrin derivatives (ZnACP and ZnTCP-C60) were used to obtain electrogenerated polymeric films on optically transparent indium tin oxide (ITO) electrodes. This approach produced stable and reproducible polymers that increase the lipophilicity of the surfaces. The spectroscopic and photodynamic properties of these polymeric films were compared with those corresponding to their porphyrin units. These materials photosensitized singlet molecular oxygen in water. Photoinactivation activity of the films were investigated in Staphylococcus aureus biofilms depositing on the surfaces. Thus, the ZnTCP-C60 film was effective in reducing bacterial survival, reaching a photokilling of 99.7 %. In addition, ZnACP-coated surface with an absorption of 0.7 at Soret band produced a notable decrease of 4 log units in cell survival (equivalent to 99.99 % reduction) upon an exposure to white light for 60 min. Confocal scanning microscopy and scanning electron microscopy were employed to detect morphological alterations. In addition to its bactericidal effects within the biofilm, PDI using ZnACP films induced cell detachment, thereby disrupting the overall architecture of the biofilm. Thus, these polymeric films emerge as compelling photodynamic active surfaces for eradicating S. aureus bacterial biofilms.
期刊介绍：
JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds.
All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor).
The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.
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