通过全基因组关联分析剖析普通小麦(Triticum aestivum L.)

IF 5.4 Q1 PLANT SCIENCES Current Plant Biology Pub Date : 2024-10-05 DOI:10.1016/j.cpb.2024.100391
Jinna Hou , Hui Deng , Fuxin Xu , Shenghui Geng , Congcong Liu , Wenxu Li , Xia Shi , Zhengqing Wu , Zhensheng Lei , Zhengfu Zhou
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引用次数: 0

摘要

面粉产品的色泽对消费者的接受程度有重要影响。在中国,面粉颜色主要由面粉白度(FW)指数决定和衡量。本研究通过全基因组关联分析,利用由来自 7 个国家的 207 个小麦(Triticum aestivum)品种组成的关联群体,对与面粉白度(FW)相关的遗传位点进行了分析。结果发现了 6 个与 FW 显著相关的数量性状位点(QTL),占表型总变异的 7.87-16.53%。从与 QTLs QFW.HAAS-1AS、QFW.HAAS-1BL、QFW.HAAS-5AL 和 QFW.HAAS-7AL 相关的单核苷酸多态性中开发出四个 KASP 标记。植物烯合成酶编码基因 TraesCS7A03G1357000(TaPsyA1)被确定为 QFW.HAAS-7AL 的候选基因。根据第二个内含子中 37 bp 的插入/缺失多态性,区分出 TaPsyA1 的两个等位基因变体(命名为 PsyA1-a 和 PsyA1-b)。PsyA1-b 包括 37 bp 插入,它导致基因的翻译框移位,并与较高的 FW 有关。PsyA1-a 等位基因缺乏 37 bp 插入,根据启动子区简单序列重复中 "TC "单位的重复数量被分为两个单倍型。在两种 PsyA1-a 单倍型中,1 型单倍型具有较高的全粉重、面粉亮度和面粉红度,较低的黄色素含量和面粉黄度。本研究开发的 KASP 标记和 PsyA1 多态性标记适合用于分子标记辅助选择,以改良小麦的 FW。
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Genetic dissection of flour whiteness through genome-wide association analysis in common wheat (Triticum aestivum L.)
The color of flour products has an important influence on consumer acceptance. Flour color is largely determined and measured by the index of flour whiteness (FW) in China. In this study, an association population comprising 207 wheat (Triticum aestivum) accessions originating from seven countries was used for dissection of FW-related genetic loci through genome-wide association analysis. Six quantitative trait loci (QTLs) significantly associated with FW were identified, accounting for 7.87–16.53 % of the total phenotypic variation. Four KASP markers were developed from single-nucleotide polymorphisms associated with the QTLs QFW.HAAS-1AS, QFW.HAAS-1BL, QFW.HAAS-5AL, and QFW.HAAS-7AL. The phytoene synthase-encoding gene TraesCS7A03G1357000 (TaPsyA1) was identified as a candidate gene for QFW.HAAS-7AL. Two allelic variants of TaPsyA1 (designated PsyA1-a and PsyA1-b) were differentiated on the basis of a 37 bp insertion/deletion polymorphism in the second intron. PsyA1-b included the 37 bp insertion, which led to a translational frameshift in the gene and was associated with higher FW. The PsyA1-a allele lacked the 37 bp insertion and was classified into two haplotypes according to the number of repeated ‘TC’ units in a simple sequence repeat in the promoter region. Of the two PsyA1-a haplotypes, the Type 1 haplotype conferred higher FW, flour brightness, and flour redness, and lower yellow pigment content and flour yellowness. The KASP markers and PsyA1 polymorphic markers developed in the present study are suitable for use in molecular marker-assisted selection for improvement of wheat FW.
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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